Isolation and characterization of SSEA-4-positive subpopulation of human deciduous dental pulp cells

Noriaki Kawanabe, Hiroaki Fukushima, Yoshihito Ishihara, Takeshi Yanagita, Hiroshi Kurosaka, Takashi Yamashiro

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objectives: It is well accepted that stage-specific embryonic antigen (SSEA)-4 is an antigen that is useful to isolate adult stem cells analogous to embryonic stem cells. Therefore, in the present study, we investigated whether SSEA-4 can also be used as a marker to identify human deciduous dental pulp (D-DP) stem cells. Materials and methods: Intact deciduous teeth were collected from healthy patients who were undergoing orthodontic treatment at Okayama University Hospital. Immunofluorescence analysis, flow cytometric analysis, and multilineage differentiation assay were performed to characterize SSEA-4+ D-DP cells. Results: The D-DP cells had the characteristics of mesenchymal stem cells (MSCs), namely plastic adherence, specific surface antigen expression, and multipotent differentiation potential. SSEA-4 expression was detected in D-DP cells in vitro and ex vivo samples. A flow cytometric analysis demonstrated that 21.2 % of the D-DP cells were positive for SSEA-4. The SSEA-4+ clonal D-DP cells showed multilineage differentiation potential toward adipocytes, osteoblasts, and chondrocytes in vitro. In fact, 26.1 % (6/23) of the SSEA-4+ clonal D-DP cells showed adipogenic potential, 91.3 % (21/23) showed osteogenic potential, 91.3 % (21/23) showed chondrogenic potential, and 87.0 % (20/23) showed both osteogenic and chondrogenic potential. Conclusions: Thus, the majority of SSEA-4+ D-DP cells had the potential for multilineage differentiation. Hence, SSEA-4 appears to be a specific marker that can be used to identify D-DP stem cells. Clinical relevance: SSEA-4+ D-DP cells appear to be a promising source of stem cells for regenerative therapy.

Original languageEnglish
Pages (from-to)363-371
Number of pages9
JournalClinical Oral Investigations
Volume19
Issue number2
DOIs
Publication statusPublished - 2015

Fingerprint

Dental Pulp
Deciduous Tooth
Stem Cells
stage-specific embryonic antigen-4
Adult Stem Cells
Surface Antigens
Embryonic Stem Cells
Chondrocytes
Cell- and Tissue-Based Therapy
Orthodontics
Osteoblasts
Mesenchymal Stromal Cells
Adipocytes
Plastics
Fluorescent Antibody Technique

Keywords

  • Adult stem cells
  • Clonal assays
  • Multilineage differentiation
  • Multipotent stem cells
  • SSEA-4+ human deciduous dental pulp stem cells

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Isolation and characterization of SSEA-4-positive subpopulation of human deciduous dental pulp cells. / Kawanabe, Noriaki; Fukushima, Hiroaki; Ishihara, Yoshihito; Yanagita, Takeshi; Kurosaka, Hiroshi; Yamashiro, Takashi.

In: Clinical Oral Investigations, Vol. 19, No. 2, 2015, p. 363-371.

Research output: Contribution to journalArticle

Kawanabe, Noriaki ; Fukushima, Hiroaki ; Ishihara, Yoshihito ; Yanagita, Takeshi ; Kurosaka, Hiroshi ; Yamashiro, Takashi. / Isolation and characterization of SSEA-4-positive subpopulation of human deciduous dental pulp cells. In: Clinical Oral Investigations. 2015 ; Vol. 19, No. 2. pp. 363-371.
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AU - Kawanabe, Noriaki

AU - Fukushima, Hiroaki

AU - Ishihara, Yoshihito

AU - Yanagita, Takeshi

AU - Kurosaka, Hiroshi

AU - Yamashiro, Takashi

PY - 2015

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N2 - Objectives: It is well accepted that stage-specific embryonic antigen (SSEA)-4 is an antigen that is useful to isolate adult stem cells analogous to embryonic stem cells. Therefore, in the present study, we investigated whether SSEA-4 can also be used as a marker to identify human deciduous dental pulp (D-DP) stem cells. Materials and methods: Intact deciduous teeth were collected from healthy patients who were undergoing orthodontic treatment at Okayama University Hospital. Immunofluorescence analysis, flow cytometric analysis, and multilineage differentiation assay were performed to characterize SSEA-4+ D-DP cells. Results: The D-DP cells had the characteristics of mesenchymal stem cells (MSCs), namely plastic adherence, specific surface antigen expression, and multipotent differentiation potential. SSEA-4 expression was detected in D-DP cells in vitro and ex vivo samples. A flow cytometric analysis demonstrated that 21.2 % of the D-DP cells were positive for SSEA-4. The SSEA-4+ clonal D-DP cells showed multilineage differentiation potential toward adipocytes, osteoblasts, and chondrocytes in vitro. In fact, 26.1 % (6/23) of the SSEA-4+ clonal D-DP cells showed adipogenic potential, 91.3 % (21/23) showed osteogenic potential, 91.3 % (21/23) showed chondrogenic potential, and 87.0 % (20/23) showed both osteogenic and chondrogenic potential. Conclusions: Thus, the majority of SSEA-4+ D-DP cells had the potential for multilineage differentiation. Hence, SSEA-4 appears to be a specific marker that can be used to identify D-DP stem cells. Clinical relevance: SSEA-4+ D-DP cells appear to be a promising source of stem cells for regenerative therapy.

AB - Objectives: It is well accepted that stage-specific embryonic antigen (SSEA)-4 is an antigen that is useful to isolate adult stem cells analogous to embryonic stem cells. Therefore, in the present study, we investigated whether SSEA-4 can also be used as a marker to identify human deciduous dental pulp (D-DP) stem cells. Materials and methods: Intact deciduous teeth were collected from healthy patients who were undergoing orthodontic treatment at Okayama University Hospital. Immunofluorescence analysis, flow cytometric analysis, and multilineage differentiation assay were performed to characterize SSEA-4+ D-DP cells. Results: The D-DP cells had the characteristics of mesenchymal stem cells (MSCs), namely plastic adherence, specific surface antigen expression, and multipotent differentiation potential. SSEA-4 expression was detected in D-DP cells in vitro and ex vivo samples. A flow cytometric analysis demonstrated that 21.2 % of the D-DP cells were positive for SSEA-4. The SSEA-4+ clonal D-DP cells showed multilineage differentiation potential toward adipocytes, osteoblasts, and chondrocytes in vitro. In fact, 26.1 % (6/23) of the SSEA-4+ clonal D-DP cells showed adipogenic potential, 91.3 % (21/23) showed osteogenic potential, 91.3 % (21/23) showed chondrogenic potential, and 87.0 % (20/23) showed both osteogenic and chondrogenic potential. Conclusions: Thus, the majority of SSEA-4+ D-DP cells had the potential for multilineage differentiation. Hence, SSEA-4 appears to be a specific marker that can be used to identify D-DP stem cells. Clinical relevance: SSEA-4+ D-DP cells appear to be a promising source of stem cells for regenerative therapy.

KW - Adult stem cells

KW - Clonal assays

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KW - Multipotent stem cells

KW - SSEA-4+ human deciduous dental pulp stem cells

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