Isolation and characterization of retinal and choroidal biomatrix

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Abstract

Retinal and choroidal biomatrices (extracellular materials) and lens capsule were isolated from bovine eyes. Water-insoluble biomatrices were collected by filtration after repeated ultrasonic disruption of the cells and treatment with EDTA. Light and electron microscopic examination showed that the isolated lens capsule, retinal and choroidal biomatrices were free of cellular, components. These biomatrices were then solubilized after pulverization in liquid nitrogen and applied to SDS-polyacrylamide gel electrophoresis. Electrophoretic patterns of the biomatrices were quite different from one another, while immunoblotting demonstrated the presence of laminin as a common constituent.

Original languageEnglish
Pages (from-to)367-374
Number of pages8
JournalJapanese Journal of Ophthalmology
Volume33
Issue number3
Publication statusPublished - Jan 1 1989

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ASJC Scopus subject areas

  • Ophthalmology

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