Abstract
Retinal and choroidal biomatrices (extracellular materials) and lens capsule were isolated from bovine eyes. Water-insoluble biomatrices were collected by filtration after repeated ultrasonic disruption of the cells and treatment with EDTA. Light and electron microscopic examination showed that the isolated lens capsule, retinal and choroidal biomatrices were free of cellular, components. These biomatrices were then solubilized after pulverization in liquid nitrogen and applied to SDS-polyacrylamide gel electrophoresis. Electrophoretic patterns of the biomatrices were quite different from one another, while immunoblotting demonstrated the presence of laminin as a common constituent.
| Original language | English |
|---|---|
| Pages (from-to) | 367-374 |
| Number of pages | 8 |
| Journal | Japanese Journal of Ophthalmology |
| Volume | 33 |
| Issue number | 3 |
| Publication status | Published - 1989 |
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ASJC Scopus subject areas
- Ophthalmology
Cite this
Isolation and characterization of retinal and choroidal biomatrix. / Matsuo, Toshihiko.
In: Japanese Journal of Ophthalmology, Vol. 33, No. 3, 1989, p. 367-374.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and characterization of retinal and choroidal biomatrix
AU - Matsuo, Toshihiko
PY - 1989
Y1 - 1989
N2 - Retinal and choroidal biomatrices (extracellular materials) and lens capsule were isolated from bovine eyes. Water-insoluble biomatrices were collected by filtration after repeated ultrasonic disruption of the cells and treatment with EDTA. Light and electron microscopic examination showed that the isolated lens capsule, retinal and choroidal biomatrices were free of cellular, components. These biomatrices were then solubilized after pulverization in liquid nitrogen and applied to SDS-polyacrylamide gel electrophoresis. Electrophoretic patterns of the biomatrices were quite different from one another, while immunoblotting demonstrated the presence of laminin as a common constituent.
AB - Retinal and choroidal biomatrices (extracellular materials) and lens capsule were isolated from bovine eyes. Water-insoluble biomatrices were collected by filtration after repeated ultrasonic disruption of the cells and treatment with EDTA. Light and electron microscopic examination showed that the isolated lens capsule, retinal and choroidal biomatrices were free of cellular, components. These biomatrices were then solubilized after pulverization in liquid nitrogen and applied to SDS-polyacrylamide gel electrophoresis. Electrophoretic patterns of the biomatrices were quite different from one another, while immunoblotting demonstrated the presence of laminin as a common constituent.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024419580&partnerID=8YFLogxK
M3 - Article
C2 - 2796015
AN - SCOPUS:0024419580
VL - 33
SP - 367
EP - 374
JO - Japanese Journal of Ophthalmology
JF - Japanese Journal of Ophthalmology
SN - 0021-5155
IS - 3
ER -