Isolation and characterization of retinal and choroidal biomatrix

Research output: Contribution to journalArticle

Abstract

Retinal and choroidal biomatrices (extracellular materials) and lens capsule were isolated from bovine eyes. Water-insoluble biomatrices were collected by filtration after repeated ultrasonic disruption of the cells and treatment with EDTA. Light and electron microscopic examination showed that the isolated lens capsule, retinal and choroidal biomatrices were free of cellular, components. These biomatrices were then solubilized after pulverization in liquid nitrogen and applied to SDS-polyacrylamide gel electrophoresis. Electrophoretic patterns of the biomatrices were quite different from one another, while immunoblotting demonstrated the presence of laminin as a common constituent.

Original languageEnglish
Pages (from-to)367-374
Number of pages8
JournalJapanese Journal of Ophthalmology
Volume33
Issue number3
Publication statusPublished - 1989

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Lenses
Capsules
Laminin
Immunoblotting
Ultrasonics
Edetic Acid
Polyacrylamide Gel Electrophoresis
Nitrogen
Electrons
Light
Water

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Isolation and characterization of retinal and choroidal biomatrix. / Matsuo, Toshihiko.

In: Japanese Journal of Ophthalmology, Vol. 33, No. 3, 1989, p. 367-374.

Research output: Contribution to journalArticle

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