Isolation and characterization of restriction endonucleases from Acidiphilium sp. 16R and 22M

Kenji Inagaki, Dexian Dou, Keiko Kita, Nobutsugu Hiraoka, Noriaki Kishimoto, Tsuyoshi Sugio, Tatsuo Tano

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Restriction endonucleases (Asp16RI and Asp22MI) have been identified from the acidophilic bacteria Acidiphilium sp. 16R and 22M. The cleavage patterns with various DNAs show that both enzymes recognize the same sequence as the PvuI restriction endonuclease (5′-CGAT ↓ CG-3′), which is from Proteus vulgaris ATCC13315. Most of the catalytic properties observed for Asp16RI and Asp22MI were similar to those observed for PvuI. However, unlike PvuI both enzymes efficiently cleaved DNA in the absence of NaCl or KCl. The purification yield of Asp22MI is 60 times that of PvuI.

Original languageEnglish
Pages (from-to)60-62
Number of pages3
JournalJournal of Fermentation and Bioengineering
Volume69
Issue number1
DOIs
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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