TY - JOUR
T1 - Isolation and characterization of restriction endonucleases from Acidiphilium sp. 16R and 22M
AU - Inagaki, Kenji
AU - Dou, Dexian
AU - Kita, Keiko
AU - Hiraoka, Nobutsugu
AU - Kishimoto, Noriaki
AU - Sugio, Tsuyoshi
AU - Tano, Tatsuo
PY - 1990
Y1 - 1990
N2 - Restriction endonucleases (Asp16RI and Asp22MI) have been identified from the acidophilic bacteria Acidiphilium sp. 16R and 22M. The cleavage patterns with various DNAs show that both enzymes recognize the same sequence as the PvuI restriction endonuclease (5′-CGAT ↓ CG-3′), which is from Proteus vulgaris ATCC13315. Most of the catalytic properties observed for Asp16RI and Asp22MI were similar to those observed for PvuI. However, unlike PvuI both enzymes efficiently cleaved DNA in the absence of NaCl or KCl. The purification yield of Asp22MI is 60 times that of PvuI.
AB - Restriction endonucleases (Asp16RI and Asp22MI) have been identified from the acidophilic bacteria Acidiphilium sp. 16R and 22M. The cleavage patterns with various DNAs show that both enzymes recognize the same sequence as the PvuI restriction endonuclease (5′-CGAT ↓ CG-3′), which is from Proteus vulgaris ATCC13315. Most of the catalytic properties observed for Asp16RI and Asp22MI were similar to those observed for PvuI. However, unlike PvuI both enzymes efficiently cleaved DNA in the absence of NaCl or KCl. The purification yield of Asp22MI is 60 times that of PvuI.
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U2 - 10.1016/0922-338X(90)90166-T
DO - 10.1016/0922-338X(90)90166-T
M3 - Article
AN - SCOPUS:0025118178
VL - 69
SP - 60
EP - 62
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 1
ER -