Restriction endonucleases (Asp16RI and Asp22MI) have been identified from the acidophilic bacteria Acidiphilium sp. 16R and 22M. The cleavage patterns with various DNAs show that both enzymes recognize the same sequence as the PvuI restriction endonuclease (5′-CGAT ↓ CG-3′), which is from Proteus vulgaris ATCC13315. Most of the catalytic properties observed for Asp16RI and Asp22MI were similar to those observed for PvuI. However, unlike PvuI both enzymes efficiently cleaved DNA in the absence of NaCl or KCl. The purification yield of Asp22MI is 60 times that of PvuI.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology