Isolation and characterization of PSI-LHCI super-complex and their sub-complexes from a red alga Cyanidioschyzon merolae

Nobuo Kamiya Lirong Tian, Zheyi Liu, Fangjun Wang, Liangliang Shen, Jinghua Chen, Lijing Chang, Songhao Zhao, Guangye Han, Wenda Wang, Tingyun Kuang, Xiaochun Qin, Jian-Ren Shen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Photosystem I (PSI)-light-harvesting complex I (LHCI) super-complex and its sub-complexes PSI core and LHCI, were purified from a unicellular red alga Cyanidioschyzon merolae and characterized. PSI-LHCI of C. merolae existed as a monomer with a molecular mass of 580 kDa. Mass spectrometry analysis identified 11 subunits (PsaA, B, C, D, E, F, I, J, K, L, O) in the core complex and three LHCI subunits, CMQ142C, CMN234C, and CMN235C in LHCI, indicating that at least three Lhcr subunits associate with the red algal PSI core. PsaG was not found in the red algae PSI-LHCI, and we suggest that the position corresponding to Lhca1 in higher plant PSI-LHCI is empty in the red algal PSI-LHCI. The PSI-LHCI complex was separated into two bands on native PAGE, suggesting that two different complexes may be present with slightly different protein compositions probably with respective to the numbers of Lhcr subunits. Based on the results obtained, a structural model was proposed for the red algal PSI-LHCI. Furthermore, pigment analysis revealed that the C. merolae PSI-LHCI contained a large amount of zeaxanthin, which is mainly associated with the LHCI complex whereas little zeaxanthin was found in the PSI core. This indicates a unique feature of the carotenoid composition of the Lhcr proteins and may suggest an important role of Zea in the light-harvesting and photoprotection of the red algal PSI-LHCI complex.

Original languageEnglish
JournalPhotosynthesis Research
DOIs
Publication statusE-pub ahead of print - Apr 12 2017
Externally publishedYes

Fingerprint

Photosystem I Protein Complex
Rhodophyta
light harvesting complex
photosystem I
Algae
Light
protein composition
zeaxanthin
Native Polyacrylamide Gel Electrophoresis
Zea
photostability
Structural Models
Molecular mass
Carotenoids
Chemical analysis
Pigments

Keywords

  • Journal Article

Cite this

Isolation and characterization of PSI-LHCI super-complex and their sub-complexes from a red alga Cyanidioschyzon merolae. / Tian, Nobuo Kamiya Lirong; Liu, Zheyi; Wang, Fangjun; Shen, Liangliang; Chen, Jinghua; Chang, Lijing; Zhao, Songhao; Han, Guangye; Wang, Wenda; Kuang, Tingyun; Qin, Xiaochun; Shen, Jian-Ren.

In: Photosynthesis Research, 12.04.2017.

Research output: Contribution to journalArticle

Tian, Nobuo Kamiya Lirong ; Liu, Zheyi ; Wang, Fangjun ; Shen, Liangliang ; Chen, Jinghua ; Chang, Lijing ; Zhao, Songhao ; Han, Guangye ; Wang, Wenda ; Kuang, Tingyun ; Qin, Xiaochun ; Shen, Jian-Ren. / Isolation and characterization of PSI-LHCI super-complex and their sub-complexes from a red alga Cyanidioschyzon merolae. In: Photosynthesis Research. 2017.
@article{3beff60df1224352aca4f6f3c829de61,
title = "Isolation and characterization of PSI-LHCI super-complex and their sub-complexes from a red alga Cyanidioschyzon merolae",
abstract = "Photosystem I (PSI)-light-harvesting complex I (LHCI) super-complex and its sub-complexes PSI core and LHCI, were purified from a unicellular red alga Cyanidioschyzon merolae and characterized. PSI-LHCI of C. merolae existed as a monomer with a molecular mass of 580 kDa. Mass spectrometry analysis identified 11 subunits (PsaA, B, C, D, E, F, I, J, K, L, O) in the core complex and three LHCI subunits, CMQ142C, CMN234C, and CMN235C in LHCI, indicating that at least three Lhcr subunits associate with the red algal PSI core. PsaG was not found in the red algae PSI-LHCI, and we suggest that the position corresponding to Lhca1 in higher plant PSI-LHCI is empty in the red algal PSI-LHCI. The PSI-LHCI complex was separated into two bands on native PAGE, suggesting that two different complexes may be present with slightly different protein compositions probably with respective to the numbers of Lhcr subunits. Based on the results obtained, a structural model was proposed for the red algal PSI-LHCI. Furthermore, pigment analysis revealed that the C. merolae PSI-LHCI contained a large amount of zeaxanthin, which is mainly associated with the LHCI complex whereas little zeaxanthin was found in the PSI core. This indicates a unique feature of the carotenoid composition of the Lhcr proteins and may suggest an important role of Zea in the light-harvesting and photoprotection of the red algal PSI-LHCI complex.",
keywords = "Journal Article",
author = "Tian, {Nobuo Kamiya Lirong} and Zheyi Liu and Fangjun Wang and Liangliang Shen and Jinghua Chen and Lijing Chang and Songhao Zhao and Guangye Han and Wenda Wang and Tingyun Kuang and Xiaochun Qin and Jian-Ren Shen",
year = "2017",
month = "4",
day = "12",
doi = "10.1007/s11120-017-0384-9",
language = "English",
journal = "Photosynthesis Research",
issn = "0166-8595",
publisher = "Springer Netherlands",

}

TY - JOUR

T1 - Isolation and characterization of PSI-LHCI super-complex and their sub-complexes from a red alga Cyanidioschyzon merolae

AU - Tian, Nobuo Kamiya Lirong

AU - Liu, Zheyi

AU - Wang, Fangjun

AU - Shen, Liangliang

AU - Chen, Jinghua

AU - Chang, Lijing

AU - Zhao, Songhao

AU - Han, Guangye

AU - Wang, Wenda

AU - Kuang, Tingyun

AU - Qin, Xiaochun

AU - Shen, Jian-Ren

PY - 2017/4/12

Y1 - 2017/4/12

N2 - Photosystem I (PSI)-light-harvesting complex I (LHCI) super-complex and its sub-complexes PSI core and LHCI, were purified from a unicellular red alga Cyanidioschyzon merolae and characterized. PSI-LHCI of C. merolae existed as a monomer with a molecular mass of 580 kDa. Mass spectrometry analysis identified 11 subunits (PsaA, B, C, D, E, F, I, J, K, L, O) in the core complex and three LHCI subunits, CMQ142C, CMN234C, and CMN235C in LHCI, indicating that at least three Lhcr subunits associate with the red algal PSI core. PsaG was not found in the red algae PSI-LHCI, and we suggest that the position corresponding to Lhca1 in higher plant PSI-LHCI is empty in the red algal PSI-LHCI. The PSI-LHCI complex was separated into two bands on native PAGE, suggesting that two different complexes may be present with slightly different protein compositions probably with respective to the numbers of Lhcr subunits. Based on the results obtained, a structural model was proposed for the red algal PSI-LHCI. Furthermore, pigment analysis revealed that the C. merolae PSI-LHCI contained a large amount of zeaxanthin, which is mainly associated with the LHCI complex whereas little zeaxanthin was found in the PSI core. This indicates a unique feature of the carotenoid composition of the Lhcr proteins and may suggest an important role of Zea in the light-harvesting and photoprotection of the red algal PSI-LHCI complex.

AB - Photosystem I (PSI)-light-harvesting complex I (LHCI) super-complex and its sub-complexes PSI core and LHCI, were purified from a unicellular red alga Cyanidioschyzon merolae and characterized. PSI-LHCI of C. merolae existed as a monomer with a molecular mass of 580 kDa. Mass spectrometry analysis identified 11 subunits (PsaA, B, C, D, E, F, I, J, K, L, O) in the core complex and three LHCI subunits, CMQ142C, CMN234C, and CMN235C in LHCI, indicating that at least three Lhcr subunits associate with the red algal PSI core. PsaG was not found in the red algae PSI-LHCI, and we suggest that the position corresponding to Lhca1 in higher plant PSI-LHCI is empty in the red algal PSI-LHCI. The PSI-LHCI complex was separated into two bands on native PAGE, suggesting that two different complexes may be present with slightly different protein compositions probably with respective to the numbers of Lhcr subunits. Based on the results obtained, a structural model was proposed for the red algal PSI-LHCI. Furthermore, pigment analysis revealed that the C. merolae PSI-LHCI contained a large amount of zeaxanthin, which is mainly associated with the LHCI complex whereas little zeaxanthin was found in the PSI core. This indicates a unique feature of the carotenoid composition of the Lhcr proteins and may suggest an important role of Zea in the light-harvesting and photoprotection of the red algal PSI-LHCI complex.

KW - Journal Article

U2 - 10.1007/s11120-017-0384-9

DO - 10.1007/s11120-017-0384-9

M3 - Article

JO - Photosynthesis Research

JF - Photosynthesis Research

SN - 0166-8595

ER -