Isolation and characterization of pepsin-soluble collagen from abalone (Haliotis discus hannai) gastropod muscle part ii

Xiuping Dong, Qixin Yuan, Hang Qi, Jingfeng Yang, Beiwei Zhu, Dayong Zhou, Yoshiyuki Murata, Wenxiu Ye

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Abalone (Haliotis discus hannai) gastropod muscle was divided into Part I (the center part of gastropod muscle) and Part II (the transforming and virginal part of gastropod muscle). Pepsin-soluble collagen was extracted from abalone gastropod muscle part II (PSCAGM-II) with yield of 8.7%. SDSPAGE and amino acid composition indicated that the PSCAGM-II contained α1 and α2 chains and might be classified as type I collagen. PSCAGM-II exhibited a maximum absorbance at 232 nm, but little absorbance near to 280 nm. FTIR investigations showed the existence of helical arrangements of collagen. PSCAGM-II triple helical stereochemical structure was destroyed when the treated temperature up to 35°C for 10 min. Denatured temperature detected by the viscometer was 22.9°C when incubated for 20 min. Study on the properties and thermal stability of PSC from abalone gastropod muscle has certain directive significance for the abalone processing industry.

Original languageEnglish
Pages (from-to)271-278
Number of pages8
JournalFood Science and Technology Research
Volume18
Issue number2
DOIs
Publication statusPublished - 2012

Keywords

  • Abalone (Haliotis discus hannai)
  • Characterization
  • Gastropod muscle
  • Pepsin-soluble collagen

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering
  • Marketing

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