Isolation and characterization of novel mutants affecting the abscisic acid sensitivity of Arabidopsis germination and seedling growth

Noriyuki Nishimura, Tomo Yoshida, Maki Murayama, Tadao Asami, Kazuo Shinozaki, Takashi Hirayama

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

To gain more insight into ABA signaling mechanisms, we conducted genetic screens searching for mutants with altered ABA response in germination and post-germination growth. We isolated seven putative ABA-hypersensitive Arabidopsis mutants and named them ABA-hypersensitive germination (ahg). These mutants exhibited diminished germination or growth ability on medium supplemented with ABA. We further studied four of them: ahg1, ahg2, ahg3 and ahg4. Mapping suggested that they were new ABA-hypersensitive loci. Characterization showed that all of them had enhanced sensitivity to salinity and high osmotic stress in germinating seeds, whereas they each had distinct sugar responses. RT-PCR experiments showed that the expression patterns of the ABA-inducible genes RABI8, AtEm1, AtEm6 and ABI5 in germinating seeds were affected by these four ahg mutations, whereas those of ABI3 and ABI4 were not. ahg4 displayed slightly increased mRNA levels of several ABA-inducible genes upon ABA treatment. By contrast, ahg1 had no clear ABA-hypersensitive phenotypes in adult plants despite its strong phenotype in germination. These results suggest that ahg1, ahg2, ahg3 and ahg4 are novel ABA-hypersensitive mutants representing distinct components in the ABA response.

Original languageEnglish
Pages (from-to)1485-1499
Number of pages15
JournalPlant and Cell Physiology
Volume45
Issue number10
Publication statusPublished - Oct 2004
Externally publishedYes

Fingerprint

Abscisic Acid
Germination
Seedlings
Arabidopsis
seedling growth
abscisic acid
germination
mutants
Growth
Seeds
Phenotype
Osmotic Pressure
Salinity
Genes
Polymerase Chain Reaction
Messenger RNA
Mutation
phenotype
osmotic stress
mature plants

Keywords

  • ABA analog
  • ABA-hypersensitive mutant
  • Germination
  • Post-germination growth
  • Stress response
  • Sugar response

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Isolation and characterization of novel mutants affecting the abscisic acid sensitivity of Arabidopsis germination and seedling growth. / Nishimura, Noriyuki; Yoshida, Tomo; Murayama, Maki; Asami, Tadao; Shinozaki, Kazuo; Hirayama, Takashi.

In: Plant and Cell Physiology, Vol. 45, No. 10, 10.2004, p. 1485-1499.

Research output: Contribution to journalArticle

Nishimura, Noriyuki ; Yoshida, Tomo ; Murayama, Maki ; Asami, Tadao ; Shinozaki, Kazuo ; Hirayama, Takashi. / Isolation and characterization of novel mutants affecting the abscisic acid sensitivity of Arabidopsis germination and seedling growth. In: Plant and Cell Physiology. 2004 ; Vol. 45, No. 10. pp. 1485-1499.
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