Isolation and characterization of microsatellite loci in Shortia rotundifolia (Diapensiaceae), an endangered relict plant on the Ryukyu Islands and Taiwan

Tomomi Dan, Yuki Mitsui, Hajime Ikeda, Yuji Isagi, Hiroaki Setoguchi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Ten microsatellite loci were isolated and characterized for Shortia rotundifolia, a relict temperate plant on the subtropical Ryukyu Islands and Taiwan. The number of alleles ranged from 2 to 21. The expected (H E) and observed (H O) heterozygosities were 0.027-0.877 and 0.027-0.622, respectively, from 74 individuals on Iriomotejima Island of Ryukyus. One locus exhibited significantly fewer heterozygotes than expected under Hardy-Weinberg equilibrium (P < 0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish conservation strategy.

Original languageEnglish
Pages (from-to)507-509
Number of pages3
JournalConservation Genetics
Volume10
Issue number3
DOIs
Publication statusPublished - Jun 1 2009
Externally publishedYes

Keywords

  • Conservation genetics
  • Heterozygosity
  • Microsatellite
  • Ryukyu Islands
  • Shortia rotundifolia

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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