Isolation and characterization of microsatellite loci in Shortia rotundifolia (Diapensiaceae), an endangered relict plant on the Ryukyu Islands and Taiwan

Tomomi Dan, Yuki Mitsui, Hajime Ikeda, Yuji Isagi, Hiroaki Setoguchi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Ten microsatellite loci were isolated and characterized for Shortia rotundifolia, a relict temperate plant on the subtropical Ryukyu Islands and Taiwan. The number of alleles ranged from 2 to 21. The expected (H E) and observed (H O) heterozygosities were 0.027-0.877 and 0.027-0.622, respectively, from 74 individuals on Iriomotejima Island of Ryukyus. One locus exhibited significantly fewer heterozygotes than expected under Hardy-Weinberg equilibrium (P <0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish conservation strategy.

Original languageEnglish
Pages (from-to)507-509
Number of pages3
JournalConservation Genetics
Volume10
Issue number3
DOIs
Publication statusPublished - Jun 2009
Externally publishedYes

Fingerprint

Diapensiaceae
Ryukyu Archipelago
Taiwan
Islands
Microsatellite Repeats
heterozygosity
microsatellite repeats
loci
Population Genetics
Heterozygote
population genetics
allele
Alleles
alleles

Keywords

  • Conservation genetics
  • Heterozygosity
  • Microsatellite
  • Ryukyu Islands
  • Shortia rotundifolia

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Isolation and characterization of microsatellite loci in Shortia rotundifolia (Diapensiaceae), an endangered relict plant on the Ryukyu Islands and Taiwan. / Dan, Tomomi; Mitsui, Yuki; Ikeda, Hajime; Isagi, Yuji; Setoguchi, Hiroaki.

In: Conservation Genetics, Vol. 10, No. 3, 06.2009, p. 507-509.

Research output: Contribution to journalArticle

@article{855a2523c4dc48d89dc84c8af52b8903,
title = "Isolation and characterization of microsatellite loci in Shortia rotundifolia (Diapensiaceae), an endangered relict plant on the Ryukyu Islands and Taiwan",
abstract = "Ten microsatellite loci were isolated and characterized for Shortia rotundifolia, a relict temperate plant on the subtropical Ryukyu Islands and Taiwan. The number of alleles ranged from 2 to 21. The expected (H E) and observed (H O) heterozygosities were 0.027-0.877 and 0.027-0.622, respectively, from 74 individuals on Iriomotejima Island of Ryukyus. One locus exhibited significantly fewer heterozygotes than expected under Hardy-Weinberg equilibrium (P <0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish conservation strategy.",
keywords = "Conservation genetics, Heterozygosity, Microsatellite, Ryukyu Islands, Shortia rotundifolia",
author = "Tomomi Dan and Yuki Mitsui and Hajime Ikeda and Yuji Isagi and Hiroaki Setoguchi",
year = "2009",
month = "6",
doi = "10.1007/s10592-008-9551-z",
language = "English",
volume = "10",
pages = "507--509",
journal = "Conservation Genetics",
issn = "1566-0621",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Isolation and characterization of microsatellite loci in Shortia rotundifolia (Diapensiaceae), an endangered relict plant on the Ryukyu Islands and Taiwan

AU - Dan, Tomomi

AU - Mitsui, Yuki

AU - Ikeda, Hajime

AU - Isagi, Yuji

AU - Setoguchi, Hiroaki

PY - 2009/6

Y1 - 2009/6

N2 - Ten microsatellite loci were isolated and characterized for Shortia rotundifolia, a relict temperate plant on the subtropical Ryukyu Islands and Taiwan. The number of alleles ranged from 2 to 21. The expected (H E) and observed (H O) heterozygosities were 0.027-0.877 and 0.027-0.622, respectively, from 74 individuals on Iriomotejima Island of Ryukyus. One locus exhibited significantly fewer heterozygotes than expected under Hardy-Weinberg equilibrium (P <0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish conservation strategy.

AB - Ten microsatellite loci were isolated and characterized for Shortia rotundifolia, a relict temperate plant on the subtropical Ryukyu Islands and Taiwan. The number of alleles ranged from 2 to 21. The expected (H E) and observed (H O) heterozygosities were 0.027-0.877 and 0.027-0.622, respectively, from 74 individuals on Iriomotejima Island of Ryukyus. One locus exhibited significantly fewer heterozygotes than expected under Hardy-Weinberg equilibrium (P <0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish conservation strategy.

KW - Conservation genetics

KW - Heterozygosity

KW - Microsatellite

KW - Ryukyu Islands

KW - Shortia rotundifolia

UR - http://www.scopus.com/inward/record.url?scp=67349089584&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67349089584&partnerID=8YFLogxK

U2 - 10.1007/s10592-008-9551-z

DO - 10.1007/s10592-008-9551-z

M3 - Article

AN - SCOPUS:67349089584

VL - 10

SP - 507

EP - 509

JO - Conservation Genetics

JF - Conservation Genetics

SN - 1566-0621

IS - 3

ER -