Isolation and characterization of lipopolysaccharide from Centipeda periodontii ATCC 35019.

Susumu Kokeguchi, O. Tsutsui, K. Kato, T. Matsumura

Research output: Contribution to journalArticle

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Abstract

Lipopolysaccharide (LPS) was extracted from cells of Centipeda periodontii ATCC 35019 by a hot phenol-water method, purified by nuclease treatment and by repeated ultracentrifugations. The LPS was composed of 21.7% hexose, 9.1% hexosamine, 10.3% fatty acid, 0.5% protein, 2.5% phosphorus, 4.3% 2-keto-3-deoxyoctonate(KDO) and 2.3% heptose. The major fatty acids of the LPS were 3OH C13:0 and C13:0. C. periodontii LPS was able to gelatinize Limulus amebocyte and to produce the local Schwartzman reaction in rabbits. LPS from C. periodontii exhibited strong immunobiological activities, mitogenicity on splenocytes of C3H/HeN mice, and stimulation of interleukin 1 (IL-1) production in peritoneal macrophages of C3H/HeN mice.

Original languageEnglish
Pages (from-to)108-112
Number of pages5
JournalOral Microbiology and Immunology
Volume5
Issue number2
Publication statusPublished - Apr 1990

Fingerprint

Lipopolysaccharides
Inbred C3H Mouse
Fatty Acids
Shwartzman Phenomenon
Heptoses
Hexosamines
Horseshoe Crabs
Hexoses
Ultracentrifugation
Peritoneal Macrophages
Phenol
Interleukin-1
Phosphorus
Rabbits
Water
Proteins

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)
  • Dentistry(all)

Cite this

Isolation and characterization of lipopolysaccharide from Centipeda periodontii ATCC 35019. / Kokeguchi, Susumu; Tsutsui, O.; Kato, K.; Matsumura, T.

In: Oral Microbiology and Immunology, Vol. 5, No. 2, 04.1990, p. 108-112.

Research output: Contribution to journalArticle

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