Abstract
Worldwide contamination by acrylamide, a neurotoxicant and carcinogen in animals, is becoming a significant problem. We isolated three novel acrylamide-degrading bacteria from domestic wastewater in Chonburi, Thailand. Using biochemical characteristics and 16S rRNA gene sequencing, the strains were identified as Klebsiella pneumoniae, Kluyvera georgiana and Enterococcus faecalis. K. georgiana strain No. 2 was selected for further characterization due to its degradation potential of high concentrations of acrylamide at the mesophilic temperatures. The strain grew well in the presence of acrylamide at concentrations to 0.5% (w/v), pH 5.0 to 7.0 and 37°C. Degradation of acrylamide to acrylic acid began after 30 min of cultivation as a biomass-dependent manner. Mass balance analysis revealed 92.3% conversion of acrylamide to acrylic acid and two lower polarity compounds. Strain No. 2 degraded many aliphatic amides but not iodoacetamide and thioacetamide. High degradation level (>80%) was found with propionamide, cyanoacetamide and acetamide. Moderate degradation was obtained in the order of formamide > butyramide > lactamide > urea while sodium azide provided 34%degradation. These findings render this novel bacterium attractive for biodegradation of acrylamide and other aliphatic amides in the environment.
| Original language | English |
|---|---|
| Pages (from-to) | 1491-1499 |
| Number of pages | 9 |
| Journal | Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering |
| Volume | 47 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Sep 1 2012 |
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Keywords
- Acrylamide
- Biodegradation
- Enterococcus faecalis
- Klebsiella pneumoniae
- Kluyvera georgiana
ASJC Scopus subject areas
- Environmental Engineering
Cite this
Isolation and characterization of Kluyvera georgiana strain with the potential for acrylamide biodegradation. / Thanyacharoen, Uthumporn; Tani, Akio; Charoenpanich, Jittima.
In: Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering, Vol. 47, No. 11, 01.09.2012, p. 1491-1499.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and characterization of Kluyvera georgiana strain with the potential for acrylamide biodegradation
AU - Thanyacharoen, Uthumporn
AU - Tani, Akio
AU - Charoenpanich, Jittima
PY - 2012/9/1
Y1 - 2012/9/1
N2 - Worldwide contamination by acrylamide, a neurotoxicant and carcinogen in animals, is becoming a significant problem. We isolated three novel acrylamide-degrading bacteria from domestic wastewater in Chonburi, Thailand. Using biochemical characteristics and 16S rRNA gene sequencing, the strains were identified as Klebsiella pneumoniae, Kluyvera georgiana and Enterococcus faecalis. K. georgiana strain No. 2 was selected for further characterization due to its degradation potential of high concentrations of acrylamide at the mesophilic temperatures. The strain grew well in the presence of acrylamide at concentrations to 0.5% (w/v), pH 5.0 to 7.0 and 37°C. Degradation of acrylamide to acrylic acid began after 30 min of cultivation as a biomass-dependent manner. Mass balance analysis revealed 92.3% conversion of acrylamide to acrylic acid and two lower polarity compounds. Strain No. 2 degraded many aliphatic amides but not iodoacetamide and thioacetamide. High degradation level (>80%) was found with propionamide, cyanoacetamide and acetamide. Moderate degradation was obtained in the order of formamide > butyramide > lactamide > urea while sodium azide provided 34%degradation. These findings render this novel bacterium attractive for biodegradation of acrylamide and other aliphatic amides in the environment.
AB - Worldwide contamination by acrylamide, a neurotoxicant and carcinogen in animals, is becoming a significant problem. We isolated three novel acrylamide-degrading bacteria from domestic wastewater in Chonburi, Thailand. Using biochemical characteristics and 16S rRNA gene sequencing, the strains were identified as Klebsiella pneumoniae, Kluyvera georgiana and Enterococcus faecalis. K. georgiana strain No. 2 was selected for further characterization due to its degradation potential of high concentrations of acrylamide at the mesophilic temperatures. The strain grew well in the presence of acrylamide at concentrations to 0.5% (w/v), pH 5.0 to 7.0 and 37°C. Degradation of acrylamide to acrylic acid began after 30 min of cultivation as a biomass-dependent manner. Mass balance analysis revealed 92.3% conversion of acrylamide to acrylic acid and two lower polarity compounds. Strain No. 2 degraded many aliphatic amides but not iodoacetamide and thioacetamide. High degradation level (>80%) was found with propionamide, cyanoacetamide and acetamide. Moderate degradation was obtained in the order of formamide > butyramide > lactamide > urea while sodium azide provided 34%degradation. These findings render this novel bacterium attractive for biodegradation of acrylamide and other aliphatic amides in the environment.
KW - Acrylamide
KW - Biodegradation
KW - Enterococcus faecalis
KW - Klebsiella pneumoniae
KW - Kluyvera georgiana
UR - http://www.scopus.com/inward/record.url?scp=84862901489&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84862901489&partnerID=8YFLogxK
U2 - 10.1080/10934529.2012.680312
DO - 10.1080/10934529.2012.680312
M3 - Article
C2 - 22702807
AN - SCOPUS:84862901489
VL - 47
SP - 1491
EP - 1499
JO - Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
JF - Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
SN - 1093-4529
IS - 11
ER -