Isolation and characterization of Kluyvera georgiana strain with the potential for acrylamide biodegradation

Uthumporn Thanyacharoen, Akio Tani, Jittima Charoenpanich

    Research output: Contribution to journalArticlepeer-review

    13 Citations (Scopus)

    Abstract

    Worldwide contamination by acrylamide, a neurotoxicant and carcinogen in animals, is becoming a significant problem. We isolated three novel acrylamide-degrading bacteria from domestic wastewater in Chonburi, Thailand. Using biochemical characteristics and 16S rRNA gene sequencing, the strains were identified as Klebsiella pneumoniae, Kluyvera georgiana and Enterococcus faecalis. K. georgiana strain No. 2 was selected for further characterization due to its degradation potential of high concentrations of acrylamide at the mesophilic temperatures. The strain grew well in the presence of acrylamide at concentrations to 0.5% (w/v), pH 5.0 to 7.0 and 37°C. Degradation of acrylamide to acrylic acid began after 30 min of cultivation as a biomass-dependent manner. Mass balance analysis revealed 92.3% conversion of acrylamide to acrylic acid and two lower polarity compounds. Strain No. 2 degraded many aliphatic amides but not iodoacetamide and thioacetamide. High degradation level (>80%) was found with propionamide, cyanoacetamide and acetamide. Moderate degradation was obtained in the order of formamide > butyramide > lactamide > urea while sodium azide provided 34%degradation. These findings render this novel bacterium attractive for biodegradation of acrylamide and other aliphatic amides in the environment.

    Original languageEnglish
    Pages (from-to)1491-1499
    Number of pages9
    JournalJournal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
    Volume47
    Issue number11
    DOIs
    Publication statusPublished - Sep 1 2012

    Keywords

    • Acrylamide
    • Biodegradation
    • Enterococcus faecalis
    • Klebsiella pneumoniae
    • Kluyvera georgiana

    ASJC Scopus subject areas

    • Environmental Engineering

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