Isolation and characterization of Kluyvera georgiana strain with the potential for acrylamide biodegradation

Uthumporn Thanyacharoen, Akio Tani, Jittima Charoenpanich

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Worldwide contamination by acrylamide, a neurotoxicant and carcinogen in animals, is becoming a significant problem. We isolated three novel acrylamide-degrading bacteria from domestic wastewater in Chonburi, Thailand. Using biochemical characteristics and 16S rRNA gene sequencing, the strains were identified as Klebsiella pneumoniae, Kluyvera georgiana and Enterococcus faecalis. K. georgiana strain No. 2 was selected for further characterization due to its degradation potential of high concentrations of acrylamide at the mesophilic temperatures. The strain grew well in the presence of acrylamide at concentrations to 0.5% (w/v), pH 5.0 to 7.0 and 37°C. Degradation of acrylamide to acrylic acid began after 30 min of cultivation as a biomass-dependent manner. Mass balance analysis revealed 92.3% conversion of acrylamide to acrylic acid and two lower polarity compounds. Strain No. 2 degraded many aliphatic amides but not iodoacetamide and thioacetamide. High degradation level (>80%) was found with propionamide, cyanoacetamide and acetamide. Moderate degradation was obtained in the order of formamide > butyramide > lactamide > urea while sodium azide provided 34%degradation. These findings render this novel bacterium attractive for biodegradation of acrylamide and other aliphatic amides in the environment.

Original languageEnglish
Pages (from-to)1491-1499
Number of pages9
JournalJournal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
Volume47
Issue number11
DOIs
Publication statusPublished - Sep 1 2012

Fingerprint

Biodegradation
Degradation
Amides
Acrylics
Bacteria
Carcinogens
Acids
Urea
Animals
Biomass
Wastewater
Contamination
Genes
Sodium
Temperature

Keywords

  • Acrylamide
  • Biodegradation
  • Enterococcus faecalis
  • Klebsiella pneumoniae
  • Kluyvera georgiana

ASJC Scopus subject areas

  • Environmental Engineering

Cite this

@article{0b07f081e9644836be0a1dd71fc966da,
title = "Isolation and characterization of Kluyvera georgiana strain with the potential for acrylamide biodegradation",
abstract = "Worldwide contamination by acrylamide, a neurotoxicant and carcinogen in animals, is becoming a significant problem. We isolated three novel acrylamide-degrading bacteria from domestic wastewater in Chonburi, Thailand. Using biochemical characteristics and 16S rRNA gene sequencing, the strains were identified as Klebsiella pneumoniae, Kluyvera georgiana and Enterococcus faecalis. K. georgiana strain No. 2 was selected for further characterization due to its degradation potential of high concentrations of acrylamide at the mesophilic temperatures. The strain grew well in the presence of acrylamide at concentrations to 0.5{\%} (w/v), pH 5.0 to 7.0 and 37°C. Degradation of acrylamide to acrylic acid began after 30 min of cultivation as a biomass-dependent manner. Mass balance analysis revealed 92.3{\%} conversion of acrylamide to acrylic acid and two lower polarity compounds. Strain No. 2 degraded many aliphatic amides but not iodoacetamide and thioacetamide. High degradation level (>80{\%}) was found with propionamide, cyanoacetamide and acetamide. Moderate degradation was obtained in the order of formamide > butyramide > lactamide > urea while sodium azide provided 34{\%}degradation. These findings render this novel bacterium attractive for biodegradation of acrylamide and other aliphatic amides in the environment.",
keywords = "Acrylamide, Biodegradation, Enterococcus faecalis, Klebsiella pneumoniae, Kluyvera georgiana",
author = "Uthumporn Thanyacharoen and Akio Tani and Jittima Charoenpanich",
year = "2012",
month = "9",
day = "1",
doi = "10.1080/10934529.2012.680312",
language = "English",
volume = "47",
pages = "1491--1499",
journal = "Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering",
issn = "1093-4529",
publisher = "Taylor and Francis Ltd.",
number = "11",

}

TY - JOUR

T1 - Isolation and characterization of Kluyvera georgiana strain with the potential for acrylamide biodegradation

AU - Thanyacharoen, Uthumporn

AU - Tani, Akio

AU - Charoenpanich, Jittima

PY - 2012/9/1

Y1 - 2012/9/1

N2 - Worldwide contamination by acrylamide, a neurotoxicant and carcinogen in animals, is becoming a significant problem. We isolated three novel acrylamide-degrading bacteria from domestic wastewater in Chonburi, Thailand. Using biochemical characteristics and 16S rRNA gene sequencing, the strains were identified as Klebsiella pneumoniae, Kluyvera georgiana and Enterococcus faecalis. K. georgiana strain No. 2 was selected for further characterization due to its degradation potential of high concentrations of acrylamide at the mesophilic temperatures. The strain grew well in the presence of acrylamide at concentrations to 0.5% (w/v), pH 5.0 to 7.0 and 37°C. Degradation of acrylamide to acrylic acid began after 30 min of cultivation as a biomass-dependent manner. Mass balance analysis revealed 92.3% conversion of acrylamide to acrylic acid and two lower polarity compounds. Strain No. 2 degraded many aliphatic amides but not iodoacetamide and thioacetamide. High degradation level (>80%) was found with propionamide, cyanoacetamide and acetamide. Moderate degradation was obtained in the order of formamide > butyramide > lactamide > urea while sodium azide provided 34%degradation. These findings render this novel bacterium attractive for biodegradation of acrylamide and other aliphatic amides in the environment.

AB - Worldwide contamination by acrylamide, a neurotoxicant and carcinogen in animals, is becoming a significant problem. We isolated three novel acrylamide-degrading bacteria from domestic wastewater in Chonburi, Thailand. Using biochemical characteristics and 16S rRNA gene sequencing, the strains were identified as Klebsiella pneumoniae, Kluyvera georgiana and Enterococcus faecalis. K. georgiana strain No. 2 was selected for further characterization due to its degradation potential of high concentrations of acrylamide at the mesophilic temperatures. The strain grew well in the presence of acrylamide at concentrations to 0.5% (w/v), pH 5.0 to 7.0 and 37°C. Degradation of acrylamide to acrylic acid began after 30 min of cultivation as a biomass-dependent manner. Mass balance analysis revealed 92.3% conversion of acrylamide to acrylic acid and two lower polarity compounds. Strain No. 2 degraded many aliphatic amides but not iodoacetamide and thioacetamide. High degradation level (>80%) was found with propionamide, cyanoacetamide and acetamide. Moderate degradation was obtained in the order of formamide > butyramide > lactamide > urea while sodium azide provided 34%degradation. These findings render this novel bacterium attractive for biodegradation of acrylamide and other aliphatic amides in the environment.

KW - Acrylamide

KW - Biodegradation

KW - Enterococcus faecalis

KW - Klebsiella pneumoniae

KW - Kluyvera georgiana

UR - http://www.scopus.com/inward/record.url?scp=84862901489&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862901489&partnerID=8YFLogxK

U2 - 10.1080/10934529.2012.680312

DO - 10.1080/10934529.2012.680312

M3 - Article

VL - 47

SP - 1491

EP - 1499

JO - Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering

JF - Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering

SN - 1093-4529

IS - 11

ER -