Isolation and characterization of human lung cancer antigens by serological screening with autologous antibodies

Tadashi Hanafusa, Ali Eldib Ali Mohamed, Kenta Kitaoka, Yoshihiro Ohue, Eiichi Nakayama, Toshiro Ono

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Serological analysis of a recombinant cDNA expression library (SEREX) derived from two lung adenocarcinoma cancer cell lines using autologous sera led to the isolation of 41 positive cDNA clones comprising 28 different antigens. They coded for a variety of nuclear and cytoplasmic proteins. Among the antigens, nucleoporin 107 (NUP107) was isolated most frequently (5 of 41 clones). The second most frequently isolated antigen was coded for by C21orf58 (4 of 41 clones). During serological analysis of selected antigens based on their reactivity to sera from normal individuals and lung cancer patients, none of the antigens showed a cancer-restricted recognition pattern. However, five genes including NUP107 showed higher expression when we examined the changes in gene expression in five different adenocarcinoma cell lines, including those used in SEREX, compared with their levels in normal lung tissues by cDNA microarray analysis. On the other hand, the expression levels of five genes including C21orf58 were down regulated in all adenocarcinoma cell lines. This SEREX study combining comprehensive gene expression assays has added to the growing list of lung cancer antigens, which may aid the development of diagnostic and immunotherapeutic reagents for patients with lung cancer.

Original languageEnglish
Pages (from-to)57-62
Number of pages6
JournalCancer Letters
Volume301
Issue number1
DOIs
Publication statusPublished - Feb 1 2011

Keywords

  • Lung adenocarcinoma
  • MYL6B
  • NUP107
  • SEREX
  • Tumor antigen

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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