Isolation and characterization of gangliosides from Theileria sergenti

Shinobu Watarai, Chihiro Sugimoto, Keiko Hosotani-Kaihara, Kazuko Kobayashi, Misao Onuma, Jin Tae Lee, Yasunori Kushi, Shizuo Handa, Tatsuji Yasuda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The gangliosides of Theileria sergenti piroplasms were isolated and analyzed by thin-layer chromatography (TLC) and TLC immunostaining test. Four species of gangliosides, designated as G-1, G-2, G-3, and G-4, were separated on TLC. G-1, G-2, G-3, and G-4 ganglioside showed the same mobility as GM3, sialosylparagloboside (SPG), i-active ganglioside, and I-active ganglioside on the TLC plate, respectively. In order to characterize the molecular species of gangliosides from T. sergenti, G-1, G-2, G-3, and G-4 gangliosides were purified and tested by TLC immunostaining test with monoclonal antibodies against gangliosides. G-1 ganglioside had reactivity to anti-GM3 monoclonal antibody. G-2 gave reaction with monoclonal antibody to SPG containing N-glycolylneuraminic acid (NeuGc). G-3 showed reactivity to the anti-i-active ganglioside (NeuGc) monoclonal antibody. G-4 was recognized by the monoclonal antibody which reacts with I-active ganglioside (NeuGc). In addition, sialic acid moiety of the gangliosides from T. sergenti piroplasms was also analyzed. N-acetylneuraminic acid-containing gangliosides were hardly detectable in T. sergenti piroplasms. Gangliosides from T. sergenti (G-1, G-2, G-3, and G-4) carried only NeuGc as their sialic acid moiety. These results suggest that G-1, G-2, G-3, and G-4 gangliosides are GM3 (NeuGc) [NeuGcα2-3Galβ1-4Glcβ1-1Cer], SPG (NeuGc) [NeuGcα2-3Gal/β1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer], i-active ganglioside (NeuGc) [NeuGcα2-3Galβ1-4GlcNAcβ1-3Gaβ1-4GlcNAcβ1-3Galβ1- 4Glcββ1-1Cer], and I-active ganglioside(NeuGc) [NeuGcα2-3Galβ1-4GlcNAcβ1-3 (Galα1-3Galβ1-4GlcNAcβ1-6) Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer], respectively.

Original languageEnglish
Pages (from-to)1099-1105
Number of pages7
JournalJournal of Veterinary Medical Science
Volume58
Issue number11
Publication statusPublished - Nov 1996

Fingerprint

Theileria sergenti
Theileria
gangliosides
Gangliosides
Thin Layer Chromatography
thin layer chromatography
monoclonal antibodies
Piroplasmida
Monoclonal Antibodies
N-Acetylneuraminic Acid
Isolation
Monoclonal antibody
sialic acids
G(M3) Ganglioside

Keywords

  • Ganglioside
  • Glycosphingolipid
  • Monoclonal antibody
  • Protozoan disease
  • Theileria sergenti

ASJC Scopus subject areas

  • Marketing
  • veterinary(all)

Cite this

Watarai, S., Sugimoto, C., Hosotani-Kaihara, K., Kobayashi, K., Onuma, M., Lee, J. T., ... Yasuda, T. (1996). Isolation and characterization of gangliosides from Theileria sergenti. Journal of Veterinary Medical Science, 58(11), 1099-1105.

Isolation and characterization of gangliosides from Theileria sergenti. / Watarai, Shinobu; Sugimoto, Chihiro; Hosotani-Kaihara, Keiko; Kobayashi, Kazuko; Onuma, Misao; Lee, Jin Tae; Kushi, Yasunori; Handa, Shizuo; Yasuda, Tatsuji.

In: Journal of Veterinary Medical Science, Vol. 58, No. 11, 11.1996, p. 1099-1105.

Research output: Contribution to journalArticle

Watarai, S, Sugimoto, C, Hosotani-Kaihara, K, Kobayashi, K, Onuma, M, Lee, JT, Kushi, Y, Handa, S & Yasuda, T 1996, 'Isolation and characterization of gangliosides from Theileria sergenti', Journal of Veterinary Medical Science, vol. 58, no. 11, pp. 1099-1105.
Watarai S, Sugimoto C, Hosotani-Kaihara K, Kobayashi K, Onuma M, Lee JT et al. Isolation and characterization of gangliosides from Theileria sergenti. Journal of Veterinary Medical Science. 1996 Nov;58(11):1099-1105.
Watarai, Shinobu ; Sugimoto, Chihiro ; Hosotani-Kaihara, Keiko ; Kobayashi, Kazuko ; Onuma, Misao ; Lee, Jin Tae ; Kushi, Yasunori ; Handa, Shizuo ; Yasuda, Tatsuji. / Isolation and characterization of gangliosides from Theileria sergenti. In: Journal of Veterinary Medical Science. 1996 ; Vol. 58, No. 11. pp. 1099-1105.
@article{7dc773937a3a49da953e2bfcbe7027e7,
title = "Isolation and characterization of gangliosides from Theileria sergenti",
abstract = "The gangliosides of Theileria sergenti piroplasms were isolated and analyzed by thin-layer chromatography (TLC) and TLC immunostaining test. Four species of gangliosides, designated as G-1, G-2, G-3, and G-4, were separated on TLC. G-1, G-2, G-3, and G-4 ganglioside showed the same mobility as GM3, sialosylparagloboside (SPG), i-active ganglioside, and I-active ganglioside on the TLC plate, respectively. In order to characterize the molecular species of gangliosides from T. sergenti, G-1, G-2, G-3, and G-4 gangliosides were purified and tested by TLC immunostaining test with monoclonal antibodies against gangliosides. G-1 ganglioside had reactivity to anti-GM3 monoclonal antibody. G-2 gave reaction with monoclonal antibody to SPG containing N-glycolylneuraminic acid (NeuGc). G-3 showed reactivity to the anti-i-active ganglioside (NeuGc) monoclonal antibody. G-4 was recognized by the monoclonal antibody which reacts with I-active ganglioside (NeuGc). In addition, sialic acid moiety of the gangliosides from T. sergenti piroplasms was also analyzed. N-acetylneuraminic acid-containing gangliosides were hardly detectable in T. sergenti piroplasms. Gangliosides from T. sergenti (G-1, G-2, G-3, and G-4) carried only NeuGc as their sialic acid moiety. These results suggest that G-1, G-2, G-3, and G-4 gangliosides are GM3 (NeuGc) [NeuGcα2-3Galβ1-4Glcβ1-1Cer], SPG (NeuGc) [NeuGcα2-3Gal/β1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer], i-active ganglioside (NeuGc) [NeuGcα2-3Galβ1-4GlcNAcβ1-3Gaβ1-4GlcNAcβ1-3Galβ1- 4Glcββ1-1Cer], and I-active ganglioside(NeuGc) [NeuGcα2-3Galβ1-4GlcNAcβ1-3 (Galα1-3Galβ1-4GlcNAcβ1-6) Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer], respectively.",
keywords = "Ganglioside, Glycosphingolipid, Monoclonal antibody, Protozoan disease, Theileria sergenti",
author = "Shinobu Watarai and Chihiro Sugimoto and Keiko Hosotani-Kaihara and Kazuko Kobayashi and Misao Onuma and Lee, {Jin Tae} and Yasunori Kushi and Shizuo Handa and Tatsuji Yasuda",
year = "1996",
month = "11",
language = "English",
volume = "58",
pages = "1099--1105",
journal = "Journal of Veterinary Medical Science",
issn = "0916-7250",
publisher = "Japanese Society of Veterinary Science",
number = "11",

}

TY - JOUR

T1 - Isolation and characterization of gangliosides from Theileria sergenti

AU - Watarai, Shinobu

AU - Sugimoto, Chihiro

AU - Hosotani-Kaihara, Keiko

AU - Kobayashi, Kazuko

AU - Onuma, Misao

AU - Lee, Jin Tae

AU - Kushi, Yasunori

AU - Handa, Shizuo

AU - Yasuda, Tatsuji

PY - 1996/11

Y1 - 1996/11

N2 - The gangliosides of Theileria sergenti piroplasms were isolated and analyzed by thin-layer chromatography (TLC) and TLC immunostaining test. Four species of gangliosides, designated as G-1, G-2, G-3, and G-4, were separated on TLC. G-1, G-2, G-3, and G-4 ganglioside showed the same mobility as GM3, sialosylparagloboside (SPG), i-active ganglioside, and I-active ganglioside on the TLC plate, respectively. In order to characterize the molecular species of gangliosides from T. sergenti, G-1, G-2, G-3, and G-4 gangliosides were purified and tested by TLC immunostaining test with monoclonal antibodies against gangliosides. G-1 ganglioside had reactivity to anti-GM3 monoclonal antibody. G-2 gave reaction with monoclonal antibody to SPG containing N-glycolylneuraminic acid (NeuGc). G-3 showed reactivity to the anti-i-active ganglioside (NeuGc) monoclonal antibody. G-4 was recognized by the monoclonal antibody which reacts with I-active ganglioside (NeuGc). In addition, sialic acid moiety of the gangliosides from T. sergenti piroplasms was also analyzed. N-acetylneuraminic acid-containing gangliosides were hardly detectable in T. sergenti piroplasms. Gangliosides from T. sergenti (G-1, G-2, G-3, and G-4) carried only NeuGc as their sialic acid moiety. These results suggest that G-1, G-2, G-3, and G-4 gangliosides are GM3 (NeuGc) [NeuGcα2-3Galβ1-4Glcβ1-1Cer], SPG (NeuGc) [NeuGcα2-3Gal/β1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer], i-active ganglioside (NeuGc) [NeuGcα2-3Galβ1-4GlcNAcβ1-3Gaβ1-4GlcNAcβ1-3Galβ1- 4Glcββ1-1Cer], and I-active ganglioside(NeuGc) [NeuGcα2-3Galβ1-4GlcNAcβ1-3 (Galα1-3Galβ1-4GlcNAcβ1-6) Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer], respectively.

AB - The gangliosides of Theileria sergenti piroplasms were isolated and analyzed by thin-layer chromatography (TLC) and TLC immunostaining test. Four species of gangliosides, designated as G-1, G-2, G-3, and G-4, were separated on TLC. G-1, G-2, G-3, and G-4 ganglioside showed the same mobility as GM3, sialosylparagloboside (SPG), i-active ganglioside, and I-active ganglioside on the TLC plate, respectively. In order to characterize the molecular species of gangliosides from T. sergenti, G-1, G-2, G-3, and G-4 gangliosides were purified and tested by TLC immunostaining test with monoclonal antibodies against gangliosides. G-1 ganglioside had reactivity to anti-GM3 monoclonal antibody. G-2 gave reaction with monoclonal antibody to SPG containing N-glycolylneuraminic acid (NeuGc). G-3 showed reactivity to the anti-i-active ganglioside (NeuGc) monoclonal antibody. G-4 was recognized by the monoclonal antibody which reacts with I-active ganglioside (NeuGc). In addition, sialic acid moiety of the gangliosides from T. sergenti piroplasms was also analyzed. N-acetylneuraminic acid-containing gangliosides were hardly detectable in T. sergenti piroplasms. Gangliosides from T. sergenti (G-1, G-2, G-3, and G-4) carried only NeuGc as their sialic acid moiety. These results suggest that G-1, G-2, G-3, and G-4 gangliosides are GM3 (NeuGc) [NeuGcα2-3Galβ1-4Glcβ1-1Cer], SPG (NeuGc) [NeuGcα2-3Gal/β1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer], i-active ganglioside (NeuGc) [NeuGcα2-3Galβ1-4GlcNAcβ1-3Gaβ1-4GlcNAcβ1-3Galβ1- 4Glcββ1-1Cer], and I-active ganglioside(NeuGc) [NeuGcα2-3Galβ1-4GlcNAcβ1-3 (Galα1-3Galβ1-4GlcNAcβ1-6) Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer], respectively.

KW - Ganglioside

KW - Glycosphingolipid

KW - Monoclonal antibody

KW - Protozoan disease

KW - Theileria sergenti

UR - http://www.scopus.com/inward/record.url?scp=0030278827&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030278827&partnerID=8YFLogxK

M3 - Article

C2 - 8959658

AN - SCOPUS:0030278827

VL - 58

SP - 1099

EP - 1105

JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

SN - 0916-7250

IS - 11

ER -