Isolation and characterization of gangliosides from Theileria sergenti

Shinobu Watarai, Chihiro Sugimoto, Keiko Hosotani-Kaihara, Kazuko Kobayashi, Misao Onuma, Jin Tae Lee, Yasunori Kushi, Shizuo Handa, Tatsuji Yasuda

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Abstract

The gangliosides of Theileria sergenti piroplasms were isolated and analyzed by thin-layer chromatography (TLC) and TLC immunostaining test. Four species of gangliosides, designated as G-1, G-2, G-3, and G-4, were separated on TLC. G-1, G-2, G-3, and G-4 ganglioside showed the same mobility as GM3, sialosylparagloboside (SPG), i-active ganglioside, and I-active ganglioside on the TLC plate, respectively. In order to characterize the molecular species of gangliosides from T. sergenti, G-1, G-2, G-3, and G-4 gangliosides were purified and tested by TLC immunostaining test with monoclonal antibodies against gangliosides. G-1 ganglioside had reactivity to anti-GM3 monoclonal antibody. G-2 gave reaction with monoclonal antibody to SPG containing N-glycolylneuraminic acid (NeuGc). G-3 showed reactivity to the anti-i-active ganglioside (NeuGc) monoclonal antibody. G-4 was recognized by the monoclonal antibody which reacts with I-active ganglioside (NeuGc). In addition, sialic acid moiety of the gangliosides from T. sergenti piroplasms was also analyzed. N-acetylneuraminic acid-containing gangliosides were hardly detectable in T. sergenti piroplasms. Gangliosides from T. sergenti (G-1, G-2, G-3, and G-4) carried only NeuGc as their sialic acid moiety. These results suggest that G-1, G-2, G-3, and G-4 gangliosides are GM3 (NeuGc) [NeuGcα2-3Galβ1-4Glcβ1-1Cer], SPG (NeuGc) [NeuGcα2-3Gal/β1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer], i-active ganglioside (NeuGc) [NeuGcα2-3Galβ1-4GlcNAcβ1-3Gaβ1-4GlcNAcβ1-3Galβ1- 4Glcββ1-1Cer], and I-active ganglioside(NeuGc) [NeuGcα2-3Galβ1-4GlcNAcβ1-3 (Galα1-3Galβ1-4GlcNAcβ1-6) Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1Cer], respectively.

Original languageEnglish
Pages (from-to)1099-1105
Number of pages7
JournalJournal of Veterinary Medical Science
Volume58
Issue number11
DOIs
Publication statusPublished - Nov 1996

Keywords

  • Ganglioside
  • Glycosphingolipid
  • Monoclonal antibody
  • Protozoan disease
  • Theileria sergenti

ASJC Scopus subject areas

  • veterinary(all)

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    Watarai, S., Sugimoto, C., Hosotani-Kaihara, K., Kobayashi, K., Onuma, M., Lee, J. T., Kushi, Y., Handa, S., & Yasuda, T. (1996). Isolation and characterization of gangliosides from Theileria sergenti. Journal of Veterinary Medical Science, 58(11), 1099-1105. https://doi.org/10.1292/jvms.58.11_1099