Isolation and characterization of FliK-independent flagellation mutants from Salmonella typhimurium

K. Kutsukake, T. Minamino, T. Yokoseki

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

A flagellum of Salmonella typhimurium and Escherichia coli consists of three structural parts, a basal body, a hook, and a filament. Because the fliK mutants produce elongated hooks, called polyhooks, lacking filament portions, the fliK gene product has been believed to be involved in both the determination of hook length and the initiation of the filament assembly. In the present study, we isolated two mutants from S. typhimurium which can form flagella even in the absence of the fliK gene product. Flagellar structures were fractionated from these suppressor mutants and inspected by electron microscopy. The suppressor mutants produced polyhook-filament complexes in the fliK mutant background, while they formed flagellar structures apparently indistinguishable from those of the wild-type strain in the fliK+ background. Genetic and sequence analyses of the suppressor mutations revealed that they are located near the 3'-end of the flhB gene, which has been believed to be involved in the early process of the basal body assembly. On the basis of these results, we discuss the mechanism of suppression of the fliK defects by the flhB mutations and propose a hypothesis on the export switching machinery of the flagellar proteins.

Original languageEnglish
Pages (from-to)7625-7629
Number of pages5
JournalJournal of Bacteriology
Volume176
Issue number24
Publication statusPublished - 1994
Externally publishedYes

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Salmonella typhimurium
Basal Bodies
Flagella
Genetic Suppression
Genes
Sequence Analysis
Electron Microscopy
Escherichia coli
Mutation
Proteins

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Isolation and characterization of FliK-independent flagellation mutants from Salmonella typhimurium. / Kutsukake, K.; Minamino, T.; Yokoseki, T.

In: Journal of Bacteriology, Vol. 176, No. 24, 1994, p. 7625-7629.

Research output: Contribution to journalArticle

Kutsukake, K, Minamino, T & Yokoseki, T 1994, 'Isolation and characterization of FliK-independent flagellation mutants from Salmonella typhimurium', Journal of Bacteriology, vol. 176, no. 24, pp. 7625-7629.
Kutsukake, K. ; Minamino, T. ; Yokoseki, T. / Isolation and characterization of FliK-independent flagellation mutants from Salmonella typhimurium. In: Journal of Bacteriology. 1994 ; Vol. 176, No. 24. pp. 7625-7629.
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