Isolation and characterization of cytochrome P450-producing bacteria from various environments

H. Ikatsu, Y. Kino, N. Kawahara, M. Adachi, Shin-ichi Miyoshi, K. I. Tomochika, S. Shinoda

Research output: Contribution to journalArticle

Abstract

Eight strains of cytochrome P450 (P450)-producing bacteria were isolated from M9 medium containing a P450-inducer as the sole carbon source from the environment. Strains EP1 to EP6 utilizing 2-ethoxyphenol as the sole carbon source were isolated from the soil of a weed-filled field, the water of a paddy field, laboratory effluent, domestic effluent and river water. Strain MP1 utilizing 2-methoxyphenol and strain CP1 utilizing camphor were isolated from oil-polluted soil and the soil of a weed-filled field, respectively. This suggests the distribution of P450-producing bacteria in the environment. Metyrapone (2-methyl-1,2-di-3-pyridyl-1-propanone) inhibited the growth of P450-producing bacteria on the media containing a P450-inducer as the sole carbon source, strongly suggesting that the P450 is involved in the catabolism of the inducer.

Original languageEnglish
Pages (from-to)111-116
Number of pages6
JournalBiocontrol Science
Volume5
Issue number2
Publication statusPublished - 2000

Fingerprint

Cytochrome P-450 Enzyme System
Bacteria
Soil
Carbon
Soils
Effluents
Camphor
Guaiacol
Metyrapone
Water
Rivers
Oils
Growth
guaethol

Keywords

  • 2-Ethoxyphenol
  • 2-Methoxyphenol
  • Bacterial cytochrome P450
  • Camphor
  • Metyrapone

ASJC Scopus subject areas

  • Environmental Engineering

Cite this

Ikatsu, H., Kino, Y., Kawahara, N., Adachi, M., Miyoshi, S., Tomochika, K. I., & Shinoda, S. (2000). Isolation and characterization of cytochrome P450-producing bacteria from various environments. Biocontrol Science, 5(2), 111-116.

Isolation and characterization of cytochrome P450-producing bacteria from various environments. / Ikatsu, H.; Kino, Y.; Kawahara, N.; Adachi, M.; Miyoshi, Shin-ichi; Tomochika, K. I.; Shinoda, S.

In: Biocontrol Science, Vol. 5, No. 2, 2000, p. 111-116.

Research output: Contribution to journalArticle

Ikatsu, H, Kino, Y, Kawahara, N, Adachi, M, Miyoshi, S, Tomochika, KI & Shinoda, S 2000, 'Isolation and characterization of cytochrome P450-producing bacteria from various environments', Biocontrol Science, vol. 5, no. 2, pp. 111-116.
Ikatsu H, Kino Y, Kawahara N, Adachi M, Miyoshi S, Tomochika KI et al. Isolation and characterization of cytochrome P450-producing bacteria from various environments. Biocontrol Science. 2000;5(2):111-116.
Ikatsu, H. ; Kino, Y. ; Kawahara, N. ; Adachi, M. ; Miyoshi, Shin-ichi ; Tomochika, K. I. ; Shinoda, S. / Isolation and characterization of cytochrome P450-producing bacteria from various environments. In: Biocontrol Science. 2000 ; Vol. 5, No. 2. pp. 111-116.
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