Isolation and characterization of cytochrome P450-producing bacteria from various environments

H. Ikatsu, Y. Kino, N. Kawahara, M. Adachi, S. I. Miyoshi, K. I. Tomochika, S. Shinoda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Eight strains of cytochrome P450 (P450)-producing bacteria were isolated from M9 medium containing a P450-inducer as the sole carbon source from the environment. Strains EP1 to EP6 utilizing 2-ethoxyphenol as the sole carbon source were isolated from the soil of a weed-filled field, the water of a paddy field, laboratory effluent, domestic effluent and river water. Strain MP1 utilizing 2-methoxyphenol and strain CP1 utilizing camphor were isolated from oil-polluted soil and the soil of a weed-filled field, respectively. This suggests the distribution of P450-producing bacteria in the environment. Metyrapone (2-methyl-1,2-di-3-pyridyl-1-propanone) inhibited the growth of P450-producing bacteria on the media containing a P450-inducer as the sole carbon source, strongly suggesting that the P450 is involved in the catabolism of the inducer.

Original languageEnglish
Pages (from-to)111-116
Number of pages6
JournalBiocontrol science
Volume5
Issue number2
DOIs
Publication statusPublished - Jan 1 2000

Keywords

  • 2-Ethoxyphenol
  • 2-Methoxyphenol
  • Bacterial cytochrome P450
  • Camphor
  • Metyrapone

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Public Health, Environmental and Occupational Health

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