Isolation and characterization of cDNA clones corresponding to the genes expressed preferentially in floral organs of Arabidopsis thaliana

Shigeko Utsugi, Wataru Sakamoto, Yutaka Ogura, Minora Murata, Fusao Motoyoshi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Seventeen cDNA clones of genes corresponding to mRNAs expressed preferentially in floral organs of Arabidopsis thaliana were obtained by differential screening of a flower bud cDNA library, and classified into five groups (1A, 17A, 1B, 4B and 5B) by cross hybridization and restriction analysis. Sequence analysis revealed that the 1A-1 and 17A-1 clones encode vegetative storage proteins (VSPs). The VSP mRNAs were detected in a small amount in leaves and increased to a limited level by wounding. Both 1B-1 and 5B-1 clones were homologous to transmembrane protein cDNAs. The protein encoded by 4B-1 clone contained a proline-rich region, but no homologous proteins were found in databases.

Original languageEnglish
Pages (from-to)759-765
Number of pages7
JournalPlant Molecular Biology
Volume32
Issue number4
Publication statusPublished - Nov 1996

Fingerprint

Arabidopsis
Arabidopsis thaliana
Complementary DNA
Clone Cells
Genes
clones
storage proteins
Proteins
genes
transmembrane proteins
Messenger RNA
cDNA libraries
proline
Gene Library
hybridization
sequence analysis
Proline
proteins
buds
Sequence Analysis

Keywords

  • Arabidopsis thaliana
  • differential screening
  • flower
  • transmembrane protein
  • vegetative storage protein (VSP)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Isolation and characterization of cDNA clones corresponding to the genes expressed preferentially in floral organs of Arabidopsis thaliana. / Utsugi, Shigeko; Sakamoto, Wataru; Ogura, Yutaka; Murata, Minora; Motoyoshi, Fusao.

In: Plant Molecular Biology, Vol. 32, No. 4, 11.1996, p. 759-765.

Research output: Contribution to journalArticle

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