Isolation and characterization of cDNA clones corresponding to the genes expressed preferentially in floral organs of Arabidopsis thaliana

Shigeko Utsugi, Wataru Sakamoto, Yutaka Ogura, Minora Murata, Fusao Motoyoshi

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Abstract

Seventeen cDNA clones of genes corresponding to mRNAs expressed preferentially in floral organs of Arabidopsis thaliana were obtained by differential screening of a flower bud cDNA library, and classified into five groups (1A, 17A, 1B, 4B and 5B) by cross hybridization and restriction analysis. Sequence analysis revealed that the 1A-1 and 17A-1 clones encode vegetative storage proteins (VSPs). The VSP mRNAs were detected in a small amount in leaves and increased to a limited level by wounding. Both 1B-1 and 5B-1 clones were homologous to transmembrane protein cDNAs. The protein encoded by 4B-1 clone contained a proline-rich region, but no homologous proteins were found in databases.

Original languageEnglish
Pages (from-to)759-765
Number of pages7
JournalPlant Molecular Biology
Volume32
Issue number4
DOIs
Publication statusPublished - Jan 1 1996

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Keywords

  • Arabidopsis thaliana
  • differential screening
  • flower
  • transmembrane protein
  • vegetative storage protein (VSP)

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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