Isolation and characterization of an amphipathic antigen from Corynebacterium diphtheriae.

Susumu Kokeguchi, K. Kato, H. Ohta, K. Fukui, M. Tsujimoto, T. Ogawa, H. Takada, S. Kotani

Research output: Contribution to journalArticle

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Abstract

Amphipathic antigen was isolated from Corynebacterium diphtheriae Park-Williams number 8 cells by extraction with 47.5% phenol, nuclease treatment and gel filtration on Sepharose 6B. The chemical composition of the amphipathic antigen was hexose (73.8%), pentose (4.6%), fatty acids (9.8%) and glycerol (4.5%). The amphipathic antigen contained arabinose and mannose as sugars at a molar ratio of 1:6 and the major fatty acids were palmitic (C16:0) acid and palmitoleic (C16:1) acid (64.2% and 26.2%, respectively). The amphipathic antigen sensitized sheep erythrocytes and had definite immunobiological activities: viz mitogenic activity on murine splenocytes, stimulatory activity on guinea pig peritoneal macrophages and human complement activation. Deacylation of the amphipathic antigen with alkali treatment lost the sheep erythrocyte-sensitizing ability and some immunobiological activities. The isolated amphipathic antigen described is not a lipoteichoic acid and is different from the teichoic acid of C. diphtheriae.

Original languageEnglish
Pages (from-to)183-199
Number of pages17
JournalMicrobios
Volume50
Issue number204-205
Publication statusPublished - 1987
Externally publishedYes

Fingerprint

Corynebacterium diphtheriae
Antigens
Sheep
Fatty Acids
Erythrocytes
Teichoic Acids
Pentoses
Arabinose
Hexoses
Complement Activation
Peritoneal Macrophages
Alkalies
Mannose
Phenol
Sepharose
Glycerol
Gel Chromatography
Guinea Pigs
Cell Count
Acids

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Kokeguchi, S., Kato, K., Ohta, H., Fukui, K., Tsujimoto, M., Ogawa, T., ... Kotani, S. (1987). Isolation and characterization of an amphipathic antigen from Corynebacterium diphtheriae. Microbios, 50(204-205), 183-199.

Isolation and characterization of an amphipathic antigen from Corynebacterium diphtheriae. / Kokeguchi, Susumu; Kato, K.; Ohta, H.; Fukui, K.; Tsujimoto, M.; Ogawa, T.; Takada, H.; Kotani, S.

In: Microbios, Vol. 50, No. 204-205, 1987, p. 183-199.

Research output: Contribution to journalArticle

Kokeguchi, S, Kato, K, Ohta, H, Fukui, K, Tsujimoto, M, Ogawa, T, Takada, H & Kotani, S 1987, 'Isolation and characterization of an amphipathic antigen from Corynebacterium diphtheriae.', Microbios, vol. 50, no. 204-205, pp. 183-199.
Kokeguchi S, Kato K, Ohta H, Fukui K, Tsujimoto M, Ogawa T et al. Isolation and characterization of an amphipathic antigen from Corynebacterium diphtheriae. Microbios. 1987;50(204-205):183-199.
Kokeguchi, Susumu ; Kato, K. ; Ohta, H. ; Fukui, K. ; Tsujimoto, M. ; Ogawa, T. ; Takada, H. ; Kotani, S. / Isolation and characterization of an amphipathic antigen from Corynebacterium diphtheriae. In: Microbios. 1987 ; Vol. 50, No. 204-205. pp. 183-199.
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