Isolation and characterization of an additional crustacean hyperglycemic hormone from the greasyback shrimp Metapenaeus ensis

Tsuyoshi Ohira, Katsuyoshi Suitoh, Fumihiro Yamane, Chiaki Nagai, Michio Suzuki, Naoaki Tsutsui, Hiromichi Nagasawa, Susumu Izumi

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1 Citation (Scopus)

Abstract

Crustacean hyperglycemic hormone (CHH) is released from the X-organ/sinus gland complex located in the eyestalks. In this study, the most abundant CHH in the sinus gland of the greasyback shrimp Metapenaeus ensis was purified by reversed-phase HPLC and identified by N-terminal amino acid sequencing. Although two CHH molecules (Mee-CHH-A and Mee-CHH-B) have already been identified from M. ensis by cDNA cloning, this study revealed the presence of an additional CHH peptide based on differences in the N-terminal amino acid sequences of the CHH-A and CHH-B. Therefore, this novel CHH was designated as Mee-CHH-C. A cDNA encoding the Mee-CHH-C precursor was cloned by RT-PCR coupled with 5′- and 3′-RACE, and it was found that the mature Mee-CHH-C consisted of 72 amino acid residues containing 6 conserved cysteine residues and possessed an amidated C terminus. Mee-CHH-C had 62 and 68% identities with Mee-CHH-A and Mee-CHH-B, respectively, and was highly homologous to CHHs characterized from other penaeid shrimp species. The hyperglycemic activity of Mee-CHH-C was examined by an in vivo bioassay using the kuruma prawn Marsupenaeus japonicus. Injection of Mee-CHH-C increased hemolymph glucose levels significantly and dose-dependently. These results indicate that Mee-CHH-C is possibly one of the major molecules in M. ensis that regulate glucose levels in the hemolymph.

Original languageEnglish
Pages (from-to)605-611
Number of pages7
JournalFisheries science
Volume76
Issue number4
DOIs
Publication statusPublished - May 18 2010

Keywords

  • Crustacea
  • Crustacean hyperglycemic hormone
  • Greasyback shrimp
  • Metapenaeus ensis
  • Sinus gland

ASJC Scopus subject areas

  • Aquatic Science

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    Ohira, T., Suitoh, K., Yamane, F., Nagai, C., Suzuki, M., Tsutsui, N., Nagasawa, H., & Izumi, S. (2010). Isolation and characterization of an additional crustacean hyperglycemic hormone from the greasyback shrimp Metapenaeus ensis. Fisheries science, 76(4), 605-611. https://doi.org/10.1007/s12562-010-0247-1