Isolation and characterization of a rheumatoid arthritis-specific antigen (RA-A47) from a human chondrocytic cell line (HCS-2/8)

Takako Hattori, Takuo Fujisawa, Kazuhiro Sasaki, Yasutaka Yutani, Tohru Nakanishi, Kojiro Takahashi, Masaharu Takigawa

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Two types of 47 kDa antigen specifically recognized by sera from rheumatoid arthritis (RA) patients were isolated from the membrane fraction of a human chondrosarcoma-derived chondrocytic cell line (HCS-2/8) by a 2-step procedure: preparative SDS-PAGE and reverse-phase HPLC. An N-terminal amino acid sequence in one of the 47 kDa antigens, named RA-A47, had 81% homology to that deduced from the DNA sequence of the colligin gene which is reported as human hsp47 gene, and 100% homology to that deduced from the DNA sequence of colligin-2 gene, a homologue of colligin. The RA-A47 cross-reacted with a monoclonal antibody raised against chick heat shock protein (Hsp) 47 and bound to gelatin. The expression of the ra-a47 gene was enhanced by heat shock treatment and TGF-β stimulation. These findings suggest that RA-A47 is a Hsp47-like protein, presumably the product of the colligin-2 gene, and that a collagen-specific molecular chaperone(s) such as Hsp47 and/or RA-A47 is involved in cartilage destruction in RA.

Original languageEnglish
Pages (from-to)679-683
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume245
Issue number3
DOIs
Publication statusPublished - Apr 28 1998

Fingerprint

Rheumatoid Arthritis
Genes
Cells
Antigens
Cell Line
DNA sequences
HSP47 Heat-Shock Proteins
Molecular Chaperones
Cartilage
Gelatin
Chondrosarcoma
Collagen
Monoclonal Antibodies
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence
Shock
Membranes
Amino Acids
Hot Temperature
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Isolation and characterization of a rheumatoid arthritis-specific antigen (RA-A47) from a human chondrocytic cell line (HCS-2/8). / Hattori, Takako; Fujisawa, Takuo; Sasaki, Kazuhiro; Yutani, Yasutaka; Nakanishi, Tohru; Takahashi, Kojiro; Takigawa, Masaharu.

In: Biochemical and Biophysical Research Communications, Vol. 245, No. 3, 28.04.1998, p. 679-683.

Research output: Contribution to journalArticle

@article{7bd67cdd537249b4bb24c3dbd3add02b,
title = "Isolation and characterization of a rheumatoid arthritis-specific antigen (RA-A47) from a human chondrocytic cell line (HCS-2/8)",
abstract = "Two types of 47 kDa antigen specifically recognized by sera from rheumatoid arthritis (RA) patients were isolated from the membrane fraction of a human chondrosarcoma-derived chondrocytic cell line (HCS-2/8) by a 2-step procedure: preparative SDS-PAGE and reverse-phase HPLC. An N-terminal amino acid sequence in one of the 47 kDa antigens, named RA-A47, had 81{\%} homology to that deduced from the DNA sequence of the colligin gene which is reported as human hsp47 gene, and 100{\%} homology to that deduced from the DNA sequence of colligin-2 gene, a homologue of colligin. The RA-A47 cross-reacted with a monoclonal antibody raised against chick heat shock protein (Hsp) 47 and bound to gelatin. The expression of the ra-a47 gene was enhanced by heat shock treatment and TGF-β stimulation. These findings suggest that RA-A47 is a Hsp47-like protein, presumably the product of the colligin-2 gene, and that a collagen-specific molecular chaperone(s) such as Hsp47 and/or RA-A47 is involved in cartilage destruction in RA.",
author = "Takako Hattori and Takuo Fujisawa and Kazuhiro Sasaki and Yasutaka Yutani and Tohru Nakanishi and Kojiro Takahashi and Masaharu Takigawa",
year = "1998",
month = "4",
day = "28",
doi = "10.1006/bbrc.1998.8505",
language = "English",
volume = "245",
pages = "679--683",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Isolation and characterization of a rheumatoid arthritis-specific antigen (RA-A47) from a human chondrocytic cell line (HCS-2/8)

AU - Hattori, Takako

AU - Fujisawa, Takuo

AU - Sasaki, Kazuhiro

AU - Yutani, Yasutaka

AU - Nakanishi, Tohru

AU - Takahashi, Kojiro

AU - Takigawa, Masaharu

PY - 1998/4/28

Y1 - 1998/4/28

N2 - Two types of 47 kDa antigen specifically recognized by sera from rheumatoid arthritis (RA) patients were isolated from the membrane fraction of a human chondrosarcoma-derived chondrocytic cell line (HCS-2/8) by a 2-step procedure: preparative SDS-PAGE and reverse-phase HPLC. An N-terminal amino acid sequence in one of the 47 kDa antigens, named RA-A47, had 81% homology to that deduced from the DNA sequence of the colligin gene which is reported as human hsp47 gene, and 100% homology to that deduced from the DNA sequence of colligin-2 gene, a homologue of colligin. The RA-A47 cross-reacted with a monoclonal antibody raised against chick heat shock protein (Hsp) 47 and bound to gelatin. The expression of the ra-a47 gene was enhanced by heat shock treatment and TGF-β stimulation. These findings suggest that RA-A47 is a Hsp47-like protein, presumably the product of the colligin-2 gene, and that a collagen-specific molecular chaperone(s) such as Hsp47 and/or RA-A47 is involved in cartilage destruction in RA.

AB - Two types of 47 kDa antigen specifically recognized by sera from rheumatoid arthritis (RA) patients were isolated from the membrane fraction of a human chondrosarcoma-derived chondrocytic cell line (HCS-2/8) by a 2-step procedure: preparative SDS-PAGE and reverse-phase HPLC. An N-terminal amino acid sequence in one of the 47 kDa antigens, named RA-A47, had 81% homology to that deduced from the DNA sequence of the colligin gene which is reported as human hsp47 gene, and 100% homology to that deduced from the DNA sequence of colligin-2 gene, a homologue of colligin. The RA-A47 cross-reacted with a monoclonal antibody raised against chick heat shock protein (Hsp) 47 and bound to gelatin. The expression of the ra-a47 gene was enhanced by heat shock treatment and TGF-β stimulation. These findings suggest that RA-A47 is a Hsp47-like protein, presumably the product of the colligin-2 gene, and that a collagen-specific molecular chaperone(s) such as Hsp47 and/or RA-A47 is involved in cartilage destruction in RA.

UR - http://www.scopus.com/inward/record.url?scp=0032576992&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032576992&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1998.8505

DO - 10.1006/bbrc.1998.8505

M3 - Article

C2 - 9588174

AN - SCOPUS:0032576992

VL - 245

SP - 679

EP - 683

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -