TY - JOUR
T1 - Isolation and characterization of a rheumatoid arthritis-specific antigen (RA-A47) from a human chondrocytic cell line (HCS-2/8)
AU - Hattori, Takako
AU - Fujisawa, Takuo
AU - Sasaki, Kazuhiro
AU - Yutani, Yasutaka
AU - Nakanishi, Tohru
AU - Takahashi, Kojiro
AU - Takigawa, Masaharu
N1 - Funding Information:
We thank Professor K. Nagata (Kyoto University, Kyoto, Japan) for kindly supplying anti-chick HSP47 antibody. This work was supported in part by Grants-in-Aid for Scienti®c Research from the Ministry of Education, Science, Sports and Culture of Japan (M.T. and T.H.), grants from the Health Science Foundation (M.T.), the Kuro-zumi Medical Foundation (K.T.), and the Ryobi-Teien Memorial Foundation (T.H.), and a Research Promotion Award of Bone and Joint Diseases from Japan Rheumatism Foundation sponsored by Hokuriku Pharmaceuticals (M.T.). We also thank Mrs. Etsuko Fujisawa for secretarial assistance.
PY - 1998/4/28
Y1 - 1998/4/28
N2 - Two types of 47 kDa antigen specifically recognized by sera from rheumatoid arthritis (RA) patients were isolated from the membrane fraction of a human chondrosarcoma-derived chondrocytic cell line (HCS-2/8) by a 2-step procedure: preparative SDS-PAGE and reverse-phase HPLC. An N-terminal amino acid sequence in one of the 47 kDa antigens, named RA-A47, had 81% homology to that deduced from the DNA sequence of the colligin gene which is reported as human hsp47 gene, and 100% homology to that deduced from the DNA sequence of colligin-2 gene, a homologue of colligin. The RA-A47 cross-reacted with a monoclonal antibody raised against chick heat shock protein (Hsp) 47 and bound to gelatin. The expression of the ra-a47 gene was enhanced by heat shock treatment and TGF-β stimulation. These findings suggest that RA-A47 is a Hsp47-like protein, presumably the product of the colligin-2 gene, and that a collagen-specific molecular chaperone(s) such as Hsp47 and/or RA-A47 is involved in cartilage destruction in RA.
AB - Two types of 47 kDa antigen specifically recognized by sera from rheumatoid arthritis (RA) patients were isolated from the membrane fraction of a human chondrosarcoma-derived chondrocytic cell line (HCS-2/8) by a 2-step procedure: preparative SDS-PAGE and reverse-phase HPLC. An N-terminal amino acid sequence in one of the 47 kDa antigens, named RA-A47, had 81% homology to that deduced from the DNA sequence of the colligin gene which is reported as human hsp47 gene, and 100% homology to that deduced from the DNA sequence of colligin-2 gene, a homologue of colligin. The RA-A47 cross-reacted with a monoclonal antibody raised against chick heat shock protein (Hsp) 47 and bound to gelatin. The expression of the ra-a47 gene was enhanced by heat shock treatment and TGF-β stimulation. These findings suggest that RA-A47 is a Hsp47-like protein, presumably the product of the colligin-2 gene, and that a collagen-specific molecular chaperone(s) such as Hsp47 and/or RA-A47 is involved in cartilage destruction in RA.
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U2 - 10.1006/bbrc.1998.8505
DO - 10.1006/bbrc.1998.8505
M3 - Article
C2 - 9588174
AN - SCOPUS:0032576992
VL - 245
SP - 679
EP - 683
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -