TY - JOUR
T1 - Isolation and characterization of a novel Staphylococcus aureus bacteriophage, φMR25, and its therapeutic potential
AU - Hoshiba, Hiroshi
AU - Uchiyama, Jumpei
AU - Kato, Shin ichiro
AU - Ujihara, Takako
AU - Muraoka, Asako
AU - Daibata, Masanori
AU - Wakiguchi, Hiroshi
AU - Matsuzaki, Shigenobu
N1 - Funding Information:
This research was partly supported by a grant from the Green Science Project, Kochi University.
PY - 2010/4
Y1 - 2010/4
N2 - A novel bacteriophage, φMR25, was isolated from a lysogenic Staphylococcus aureus strain by mitomycin C induction. Its biological features were analyzed in comparison with φMR11, which was described previously as a prototype therapeutic phage. φMR25 is morphologically similar to φMR11 (morphotype B1 of family Myoviridae) but has a broader host range than φMR11 on S. aureus strains. φMR25 can also multiply on S. aureus lysogens of φMR11. Its DNA is 44,342 bp in size, is predicted to include 70 open reading frames, and does not contain genes related to toxin or drug resistance. The lysogenic module and most of the putative virion protein genes are completely different from those of φMR11. In spite of their genetic diversity, intraperitoneal administration of φMR25 rescued mice inoculated with a lethal dose of S. aureus, as was the case for φMR11. These results suggest that φMR25 could be another candidate phage to treat S. aureus infection.
AB - A novel bacteriophage, φMR25, was isolated from a lysogenic Staphylococcus aureus strain by mitomycin C induction. Its biological features were analyzed in comparison with φMR11, which was described previously as a prototype therapeutic phage. φMR25 is morphologically similar to φMR11 (morphotype B1 of family Myoviridae) but has a broader host range than φMR11 on S. aureus strains. φMR25 can also multiply on S. aureus lysogens of φMR11. Its DNA is 44,342 bp in size, is predicted to include 70 open reading frames, and does not contain genes related to toxin or drug resistance. The lysogenic module and most of the putative virion protein genes are completely different from those of φMR11. In spite of their genetic diversity, intraperitoneal administration of φMR25 rescued mice inoculated with a lethal dose of S. aureus, as was the case for φMR11. These results suggest that φMR25 could be another candidate phage to treat S. aureus infection.
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U2 - 10.1007/s00705-010-0623-2
DO - 10.1007/s00705-010-0623-2
M3 - Article
C2 - 20224894
AN - SCOPUS:77950847429
SN - 0304-8608
VL - 155
SP - 545
EP - 552
JO - Archives of Virology
JF - Archives of Virology
IS - 4
ER -