Isolation and characterization of a new dichloromethane degrading bacterium, Ralstonia metallidurans, PD11

Chizuko Miyake-Nakayama, Sachiyo Masujima, Hisayoshi Ikatsu, Shin-ichi Miyoshi, Sumio Shinoda

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The widely used organic solvent dichloromethane (DCM) has various toxic effects including carcinogenesis. We isolated a DCM-degrading bacterium Ralstonia metallidurans PD11 from drainage water which grew with DCM as a sole carbon source. PD11 was a methylotrophic bacterium with the ability to grow with C1 compounds such as methanol or methylamine. Although the existence of methylotrophic bacteria having DCM-degrading ability has been reported, there has been no report on Ralstonia sp. to date. The DCM-degrading activity of PD11 was increased by acclimatization, finally reaching a level to degrade 2,500 mg DCM/l within a week. The cell-free extract of PD11 showed DCM-degrading activity by liberating chloride which was stimulated by addition of glutathione, suggesting that the DCM dehalogenationg enzyme could be classified into the glutathione S-transferase super family.

Original languageEnglish
Pages (from-to)89-93
Number of pages5
JournalBiocontrol Science
Volume9
Issue number4
Publication statusPublished - Dec 2004

Fingerprint

Ralstonia
Methylene Chloride
Dichloromethane
Bacteria
Poisons
Acclimatization
Cell Extracts
Glutathione Transferase
Organic solvents
Drainage
Glutathione
Methanol
Chlorides
Carcinogenesis
Carbon
Enzymes

Keywords

  • Biodegradation
  • Dichloromethane
  • Methylotroph

ASJC Scopus subject areas

  • Environmental Engineering

Cite this

Isolation and characterization of a new dichloromethane degrading bacterium, Ralstonia metallidurans, PD11. / Miyake-Nakayama, Chizuko; Masujima, Sachiyo; Ikatsu, Hisayoshi; Miyoshi, Shin-ichi; Shinoda, Sumio.

In: Biocontrol Science, Vol. 9, No. 4, 12.2004, p. 89-93.

Research output: Contribution to journalArticle

Miyake-Nakayama, C, Masujima, S, Ikatsu, H, Miyoshi, S & Shinoda, S 2004, 'Isolation and characterization of a new dichloromethane degrading bacterium, Ralstonia metallidurans, PD11', Biocontrol Science, vol. 9, no. 4, pp. 89-93.
Miyake-Nakayama, Chizuko ; Masujima, Sachiyo ; Ikatsu, Hisayoshi ; Miyoshi, Shin-ichi ; Shinoda, Sumio. / Isolation and characterization of a new dichloromethane degrading bacterium, Ralstonia metallidurans, PD11. In: Biocontrol Science. 2004 ; Vol. 9, No. 4. pp. 89-93.
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