Abstract
The widely used organic solvent dichloromethane (DCM) has various toxic effects including carcinogenesis. We isolated a DCM-degrading bacterium Ralstonia metallidurans PD11 from drainage water which grew with DCM as a sole carbon source. PD11 was a methylotrophic bacterium with the ability to grow with C1 compounds such as methanol or methylamine. Although the existence of methylotrophic bacteria having DCM-degrading ability has been reported, there has been no report on Ralstonia sp. to date. The DCM-degrading activity of PD11 was increased by acclimatization, finally reaching a level to degrade 2,500 mg DCM/l within a week. The cell-free extract of PD11 showed DCM-degrading activity by liberating chloride which was stimulated by addition of glutathione, suggesting that the DCM dehalogenationg enzyme could be classified into the glutathione S-transferase super family.
| Original language | English |
|---|---|
| Pages (from-to) | 89-93 |
| Number of pages | 5 |
| Journal | Biocontrol Science |
| Volume | 9 |
| Issue number | 4 |
| Publication status | Published - Dec 2004 |
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Keywords
- Biodegradation
- Dichloromethane
- Methylotroph
ASJC Scopus subject areas
- Environmental Engineering
Cite this
Isolation and characterization of a new dichloromethane degrading bacterium, Ralstonia metallidurans, PD11. / Miyake-Nakayama, Chizuko; Masujima, Sachiyo; Ikatsu, Hisayoshi; Miyoshi, Shin-ichi; Shinoda, Sumio.
In: Biocontrol Science, Vol. 9, No. 4, 12.2004, p. 89-93.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and characterization of a new dichloromethane degrading bacterium, Ralstonia metallidurans, PD11
AU - Miyake-Nakayama, Chizuko
AU - Masujima, Sachiyo
AU - Ikatsu, Hisayoshi
AU - Miyoshi, Shin-ichi
AU - Shinoda, Sumio
PY - 2004/12
Y1 - 2004/12
N2 - The widely used organic solvent dichloromethane (DCM) has various toxic effects including carcinogenesis. We isolated a DCM-degrading bacterium Ralstonia metallidurans PD11 from drainage water which grew with DCM as a sole carbon source. PD11 was a methylotrophic bacterium with the ability to grow with C1 compounds such as methanol or methylamine. Although the existence of methylotrophic bacteria having DCM-degrading ability has been reported, there has been no report on Ralstonia sp. to date. The DCM-degrading activity of PD11 was increased by acclimatization, finally reaching a level to degrade 2,500 mg DCM/l within a week. The cell-free extract of PD11 showed DCM-degrading activity by liberating chloride which was stimulated by addition of glutathione, suggesting that the DCM dehalogenationg enzyme could be classified into the glutathione S-transferase super family.
AB - The widely used organic solvent dichloromethane (DCM) has various toxic effects including carcinogenesis. We isolated a DCM-degrading bacterium Ralstonia metallidurans PD11 from drainage water which grew with DCM as a sole carbon source. PD11 was a methylotrophic bacterium with the ability to grow with C1 compounds such as methanol or methylamine. Although the existence of methylotrophic bacteria having DCM-degrading ability has been reported, there has been no report on Ralstonia sp. to date. The DCM-degrading activity of PD11 was increased by acclimatization, finally reaching a level to degrade 2,500 mg DCM/l within a week. The cell-free extract of PD11 showed DCM-degrading activity by liberating chloride which was stimulated by addition of glutathione, suggesting that the DCM dehalogenationg enzyme could be classified into the glutathione S-transferase super family.
KW - Biodegradation
KW - Dichloromethane
KW - Methylotroph
UR - http://www.scopus.com/inward/record.url?scp=13244272412&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=13244272412&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:13244272412
VL - 9
SP - 89
EP - 93
JO - Biocontrol Science
JF - Biocontrol Science
SN - 1342-4815
IS - 4
ER -