Abstract
The widely used organic solvent dichloromethane (DCM) has various toxic effects including carcinogenesis. We isolated a DCM-degrading bacterium Ralstonia metallidurans PD11 from drainage water which grew with DCM as a sole carbon source. PD11 was a methylotrophic bacterium with the ability to grow with C1 compounds such as methanol or methylamine. Although the existence of methylotrophic bacteria having DCM-degrading ability has been reported, there has been no report on Ralstonia sp. to date. The DCM-degrading activity of PD11 was increased by acclimatization, finally reaching a level to degrade 2,500 mg DCM/l within a week. The cell-free extract of PD11 showed DCM-degrading activity by liberating chloride which was stimulated by addition of glutathione, suggesting that the DCM dehalogenationg enzyme could be classified into the glutathione S-transferase super family.
Original language | English |
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Pages (from-to) | 89-93 |
Number of pages | 5 |
Journal | Biocontrol science |
Volume | 9 |
Issue number | 4 |
DOIs | |
Publication status | Published - Dec 2004 |
Keywords
- Biodegradation
- Dichloromethane
- Methylotroph
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Public Health, Environmental and Occupational Health