Isolation and characterization of a bovine neural specific protein (CRMP-2) cDNA homologous to unc-33, a C. elegans gene implicated in axonal outgrowth and guidance

Tohru Kamata, Marianne Subleski, Yoshinobu Hara, Naoya Yuhki, Hsiang fu Kung, Neal G. Copeland, Nancy A. Jenkins, Teizo Yoshimura, William Modi, Terry D. Copeland

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

We have cloned the cDNA encoding bovine CRMP-2 from bovine brains. A full length cDNA encoding bovine CRMP-2 was isolated and sequenced. The deduced amino acid sequence reveals that the gene encodes a protein of 572 amino acids and is highly homologous to Caenorhabitis elegans unc-33, which controls the guidance and outgrowth of neuronal axons. The CRMP-2 transcript was present in bovine brains but not non-neural tissues, and its protein product existed in both soluble and membrane-bound forms. The expression of CRMP-2 protein and mRNA was upregulated during neuronal differentiation of rat PC12 cells. Immunoprecipitation of PC12 cell extracts shows that CRMP-2 was co-immunoprecipitated with a 190 kDa protein (p190). Both CRMP-2 and p190 were phosphorylated on serine residues in vivo and in vitro in a kinase assay of CRMP-2 immune complexes. Immunocytochemistry shows that CRMP-2 was exclusively localized in both the central and peripheral nervous systems in mouse embryos and detectable in the adult brain although the level of CRMP-2 decreased. The protein was expressed in the axon, dendrite, and cytoplasm of postmitotic neurons and in the cytoplasm of oligodendrocytes and astrocytes. The CRMP-2 gene maps to the region of mouse chromosome 14 syntenic with human chromosome 8p21. Taken together, these data suggest multi-functional roles for CRMP-2 in developing and adult nervous systems, and the biological activity of CRMP-2 could be regulated by phosphorylation reaction.

Original languageEnglish
Pages (from-to)219-236
Number of pages18
JournalMolecular Brain Research
Volume54
Issue number2
DOIs
Publication statusPublished - Mar 1 1998
Externally publishedYes

Keywords

  • Bovine CRMP-2
  • Chromosome mapping
  • Neural development
  • Protein kinase
  • unc-33

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Isolation and characterization of a bovine neural specific protein (CRMP-2) cDNA homologous to unc-33, a C. elegans gene implicated in axonal outgrowth and guidance'. Together they form a unique fingerprint.

  • Cite this

    Kamata, T., Subleski, M., Hara, Y., Yuhki, N., Kung, H. F., Copeland, N. G., Jenkins, N. A., Yoshimura, T., Modi, W., & Copeland, T. D. (1998). Isolation and characterization of a bovine neural specific protein (CRMP-2) cDNA homologous to unc-33, a C. elegans gene implicated in axonal outgrowth and guidance. Molecular Brain Research, 54(2), 219-236. https://doi.org/10.1016/S0169-328X(97)00332-X