Ischemic Pre-Conditioning Enhances the Mobilization and Recruitment of Bone Marrow Stem Cells to Protect Against Ischemia/Reperfusion Injury in the Late Phase

Takahiro Kamota, Tao Sheng Li, Noriyasu Morikage, Masanori Murakami, Mako Ohshima, Masayuki Kubo, Toshiro Kobayashi, Akihito Mikamo, Yasuhiro Ikeda, Masunori Matsuzaki, Kimikazu Hamano

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Objectives: The aim of this study was to investigate whether the mobilization and recruitment of bone marrow stem cells (BMSCs) contribute to cardioprotection in the late phase after ischemic pre-conditioning (IPC). Background: IPC is an innate phenomenon in which brief exposure to sublethal ischemia provides tissue protection from subsequent ischemia/reperfusion (I/R) injury. A delayed cardioprotection also occurs after IPC, but the precise mechanism is unclear. Methods: IPC was created with 4 cycles of 5-min occlusion and reperfusion of the abdominal aorta in mice. Heart I/R injury was induced by occluding the left anterior descending artery for 30 min immediately (early phase) or 24 h (late phase) after IPC. Results: Serum vascular endothelial growth factor and stromal cell-derived factor-1α levels were increased significantly 1 and 3 h after IPC, but CD34+ and CD34+/flk-1+ stem cells in the peripheral blood were increased significantly 12 and 24 h after IPC (p <0.05). Compared with the control treatment, both the early and late phases of IPC protected the heart against I/R injury. However, the recruitment of BMSCs was significantly greater in the heart when I/R injury was induced in late phase than in the early phase of IPC (p <0.01). Interestingly, the blockade of the recruitment of BMSCs significantly attenuated the cardioprotective effect of IPC in the late phase (p <0.01) but did not change in the early phase. Conclusions: Cardioprotection was observed in the early and late phases of IPC; however, the enhanced mobilization and recruitment of BMSCs played an important role in the late phase of IPC.

Original languageEnglish
Pages (from-to)1814-1822
Number of pages9
JournalJournal of the American College of Cardiology
Volume53
Issue number19
DOIs
Publication statusPublished - May 12 2009
Externally publishedYes

Fingerprint

Ischemic Preconditioning
Reperfusion Injury
Bone Marrow Cells
Stem Cells
Chemokine CXCL12
Abdominal Aorta
Vascular Endothelial Growth Factor A
Reperfusion
Ischemia
Arteries

Keywords

  • bone marrow stem cell
  • ischemic pre-conditioning
  • late phase
  • mobilization
  • recruitment

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Ischemic Pre-Conditioning Enhances the Mobilization and Recruitment of Bone Marrow Stem Cells to Protect Against Ischemia/Reperfusion Injury in the Late Phase. / Kamota, Takahiro; Li, Tao Sheng; Morikage, Noriyasu; Murakami, Masanori; Ohshima, Mako; Kubo, Masayuki; Kobayashi, Toshiro; Mikamo, Akihito; Ikeda, Yasuhiro; Matsuzaki, Masunori; Hamano, Kimikazu.

In: Journal of the American College of Cardiology, Vol. 53, No. 19, 12.05.2009, p. 1814-1822.

Research output: Contribution to journalArticle

Kamota, T, Li, TS, Morikage, N, Murakami, M, Ohshima, M, Kubo, M, Kobayashi, T, Mikamo, A, Ikeda, Y, Matsuzaki, M & Hamano, K 2009, 'Ischemic Pre-Conditioning Enhances the Mobilization and Recruitment of Bone Marrow Stem Cells to Protect Against Ischemia/Reperfusion Injury in the Late Phase', Journal of the American College of Cardiology, vol. 53, no. 19, pp. 1814-1822. https://doi.org/10.1016/j.jacc.2009.02.015
Kamota, Takahiro ; Li, Tao Sheng ; Morikage, Noriyasu ; Murakami, Masanori ; Ohshima, Mako ; Kubo, Masayuki ; Kobayashi, Toshiro ; Mikamo, Akihito ; Ikeda, Yasuhiro ; Matsuzaki, Masunori ; Hamano, Kimikazu. / Ischemic Pre-Conditioning Enhances the Mobilization and Recruitment of Bone Marrow Stem Cells to Protect Against Ischemia/Reperfusion Injury in the Late Phase. In: Journal of the American College of Cardiology. 2009 ; Vol. 53, No. 19. pp. 1814-1822.
@article{320451fe44204326b04b8e494d2237de,
title = "Ischemic Pre-Conditioning Enhances the Mobilization and Recruitment of Bone Marrow Stem Cells to Protect Against Ischemia/Reperfusion Injury in the Late Phase",
abstract = "Objectives: The aim of this study was to investigate whether the mobilization and recruitment of bone marrow stem cells (BMSCs) contribute to cardioprotection in the late phase after ischemic pre-conditioning (IPC). Background: IPC is an innate phenomenon in which brief exposure to sublethal ischemia provides tissue protection from subsequent ischemia/reperfusion (I/R) injury. A delayed cardioprotection also occurs after IPC, but the precise mechanism is unclear. Methods: IPC was created with 4 cycles of 5-min occlusion and reperfusion of the abdominal aorta in mice. Heart I/R injury was induced by occluding the left anterior descending artery for 30 min immediately (early phase) or 24 h (late phase) after IPC. Results: Serum vascular endothelial growth factor and stromal cell-derived factor-1α levels were increased significantly 1 and 3 h after IPC, but CD34+ and CD34+/flk-1+ stem cells in the peripheral blood were increased significantly 12 and 24 h after IPC (p <0.05). Compared with the control treatment, both the early and late phases of IPC protected the heart against I/R injury. However, the recruitment of BMSCs was significantly greater in the heart when I/R injury was induced in late phase than in the early phase of IPC (p <0.01). Interestingly, the blockade of the recruitment of BMSCs significantly attenuated the cardioprotective effect of IPC in the late phase (p <0.01) but did not change in the early phase. Conclusions: Cardioprotection was observed in the early and late phases of IPC; however, the enhanced mobilization and recruitment of BMSCs played an important role in the late phase of IPC.",
keywords = "bone marrow stem cell, ischemic pre-conditioning, late phase, mobilization, recruitment",
author = "Takahiro Kamota and Li, {Tao Sheng} and Noriyasu Morikage and Masanori Murakami and Mako Ohshima and Masayuki Kubo and Toshiro Kobayashi and Akihito Mikamo and Yasuhiro Ikeda and Masunori Matsuzaki and Kimikazu Hamano",
year = "2009",
month = "5",
day = "12",
doi = "10.1016/j.jacc.2009.02.015",
language = "English",
volume = "53",
pages = "1814--1822",
journal = "Journal of the American College of Cardiology",
issn = "0735-1097",
publisher = "Elsevier USA",
number = "19",

}

TY - JOUR

T1 - Ischemic Pre-Conditioning Enhances the Mobilization and Recruitment of Bone Marrow Stem Cells to Protect Against Ischemia/Reperfusion Injury in the Late Phase

AU - Kamota, Takahiro

AU - Li, Tao Sheng

AU - Morikage, Noriyasu

AU - Murakami, Masanori

AU - Ohshima, Mako

AU - Kubo, Masayuki

AU - Kobayashi, Toshiro

AU - Mikamo, Akihito

AU - Ikeda, Yasuhiro

AU - Matsuzaki, Masunori

AU - Hamano, Kimikazu

PY - 2009/5/12

Y1 - 2009/5/12

N2 - Objectives: The aim of this study was to investigate whether the mobilization and recruitment of bone marrow stem cells (BMSCs) contribute to cardioprotection in the late phase after ischemic pre-conditioning (IPC). Background: IPC is an innate phenomenon in which brief exposure to sublethal ischemia provides tissue protection from subsequent ischemia/reperfusion (I/R) injury. A delayed cardioprotection also occurs after IPC, but the precise mechanism is unclear. Methods: IPC was created with 4 cycles of 5-min occlusion and reperfusion of the abdominal aorta in mice. Heart I/R injury was induced by occluding the left anterior descending artery for 30 min immediately (early phase) or 24 h (late phase) after IPC. Results: Serum vascular endothelial growth factor and stromal cell-derived factor-1α levels were increased significantly 1 and 3 h after IPC, but CD34+ and CD34+/flk-1+ stem cells in the peripheral blood were increased significantly 12 and 24 h after IPC (p <0.05). Compared with the control treatment, both the early and late phases of IPC protected the heart against I/R injury. However, the recruitment of BMSCs was significantly greater in the heart when I/R injury was induced in late phase than in the early phase of IPC (p <0.01). Interestingly, the blockade of the recruitment of BMSCs significantly attenuated the cardioprotective effect of IPC in the late phase (p <0.01) but did not change in the early phase. Conclusions: Cardioprotection was observed in the early and late phases of IPC; however, the enhanced mobilization and recruitment of BMSCs played an important role in the late phase of IPC.

AB - Objectives: The aim of this study was to investigate whether the mobilization and recruitment of bone marrow stem cells (BMSCs) contribute to cardioprotection in the late phase after ischemic pre-conditioning (IPC). Background: IPC is an innate phenomenon in which brief exposure to sublethal ischemia provides tissue protection from subsequent ischemia/reperfusion (I/R) injury. A delayed cardioprotection also occurs after IPC, but the precise mechanism is unclear. Methods: IPC was created with 4 cycles of 5-min occlusion and reperfusion of the abdominal aorta in mice. Heart I/R injury was induced by occluding the left anterior descending artery for 30 min immediately (early phase) or 24 h (late phase) after IPC. Results: Serum vascular endothelial growth factor and stromal cell-derived factor-1α levels were increased significantly 1 and 3 h after IPC, but CD34+ and CD34+/flk-1+ stem cells in the peripheral blood were increased significantly 12 and 24 h after IPC (p <0.05). Compared with the control treatment, both the early and late phases of IPC protected the heart against I/R injury. However, the recruitment of BMSCs was significantly greater in the heart when I/R injury was induced in late phase than in the early phase of IPC (p <0.01). Interestingly, the blockade of the recruitment of BMSCs significantly attenuated the cardioprotective effect of IPC in the late phase (p <0.01) but did not change in the early phase. Conclusions: Cardioprotection was observed in the early and late phases of IPC; however, the enhanced mobilization and recruitment of BMSCs played an important role in the late phase of IPC.

KW - bone marrow stem cell

KW - ischemic pre-conditioning

KW - late phase

KW - mobilization

KW - recruitment

UR - http://www.scopus.com/inward/record.url?scp=65349114240&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65349114240&partnerID=8YFLogxK

U2 - 10.1016/j.jacc.2009.02.015

DO - 10.1016/j.jacc.2009.02.015

M3 - Article

C2 - 19422991

AN - SCOPUS:65349114240

VL - 53

SP - 1814

EP - 1822

JO - Journal of the American College of Cardiology

JF - Journal of the American College of Cardiology

SN - 0735-1097

IS - 19

ER -