Abstract
Objectives: The aim of this study was to investigate whether the mobilization and recruitment of bone marrow stem cells (BMSCs) contribute to cardioprotection in the late phase after ischemic pre-conditioning (IPC). Background: IPC is an innate phenomenon in which brief exposure to sublethal ischemia provides tissue protection from subsequent ischemia/reperfusion (I/R) injury. A delayed cardioprotection also occurs after IPC, but the precise mechanism is unclear. Methods: IPC was created with 4 cycles of 5-min occlusion and reperfusion of the abdominal aorta in mice. Heart I/R injury was induced by occluding the left anterior descending artery for 30 min immediately (early phase) or 24 h (late phase) after IPC. Results: Serum vascular endothelial growth factor and stromal cell-derived factor-1α levels were increased significantly 1 and 3 h after IPC, but CD34+ and CD34+/flk-1+ stem cells in the peripheral blood were increased significantly 12 and 24 h after IPC (p <0.05). Compared with the control treatment, both the early and late phases of IPC protected the heart against I/R injury. However, the recruitment of BMSCs was significantly greater in the heart when I/R injury was induced in late phase than in the early phase of IPC (p <0.01). Interestingly, the blockade of the recruitment of BMSCs significantly attenuated the cardioprotective effect of IPC in the late phase (p <0.01) but did not change in the early phase. Conclusions: Cardioprotection was observed in the early and late phases of IPC; however, the enhanced mobilization and recruitment of BMSCs played an important role in the late phase of IPC.
| Original language | English |
|---|---|
| Pages (from-to) | 1814-1822 |
| Number of pages | 9 |
| Journal | Journal of the American College of Cardiology |
| Volume | 53 |
| Issue number | 19 |
| DOIs | |
| Publication status | Published - May 12 2009 |
| Externally published | Yes |
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Keywords
- bone marrow stem cell
- ischemic pre-conditioning
- late phase
- mobilization
- recruitment
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Ischemic Pre-Conditioning Enhances the Mobilization and Recruitment of Bone Marrow Stem Cells to Protect Against Ischemia/Reperfusion Injury in the Late Phase. / Kamota, Takahiro; Li, Tao Sheng; Morikage, Noriyasu; Murakami, Masanori; Ohshima, Mako; Kubo, Masayuki; Kobayashi, Toshiro; Mikamo, Akihito; Ikeda, Yasuhiro; Matsuzaki, Masunori; Hamano, Kimikazu.
In: Journal of the American College of Cardiology, Vol. 53, No. 19, 12.05.2009, p. 1814-1822.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ischemic Pre-Conditioning Enhances the Mobilization and Recruitment of Bone Marrow Stem Cells to Protect Against Ischemia/Reperfusion Injury in the Late Phase
AU - Kamota, Takahiro
AU - Li, Tao Sheng
AU - Morikage, Noriyasu
AU - Murakami, Masanori
AU - Ohshima, Mako
AU - Kubo, Masayuki
AU - Kobayashi, Toshiro
AU - Mikamo, Akihito
AU - Ikeda, Yasuhiro
AU - Matsuzaki, Masunori
AU - Hamano, Kimikazu
PY - 2009/5/12
Y1 - 2009/5/12
N2 - Objectives: The aim of this study was to investigate whether the mobilization and recruitment of bone marrow stem cells (BMSCs) contribute to cardioprotection in the late phase after ischemic pre-conditioning (IPC). Background: IPC is an innate phenomenon in which brief exposure to sublethal ischemia provides tissue protection from subsequent ischemia/reperfusion (I/R) injury. A delayed cardioprotection also occurs after IPC, but the precise mechanism is unclear. Methods: IPC was created with 4 cycles of 5-min occlusion and reperfusion of the abdominal aorta in mice. Heart I/R injury was induced by occluding the left anterior descending artery for 30 min immediately (early phase) or 24 h (late phase) after IPC. Results: Serum vascular endothelial growth factor and stromal cell-derived factor-1α levels were increased significantly 1 and 3 h after IPC, but CD34+ and CD34+/flk-1+ stem cells in the peripheral blood were increased significantly 12 and 24 h after IPC (p <0.05). Compared with the control treatment, both the early and late phases of IPC protected the heart against I/R injury. However, the recruitment of BMSCs was significantly greater in the heart when I/R injury was induced in late phase than in the early phase of IPC (p <0.01). Interestingly, the blockade of the recruitment of BMSCs significantly attenuated the cardioprotective effect of IPC in the late phase (p <0.01) but did not change in the early phase. Conclusions: Cardioprotection was observed in the early and late phases of IPC; however, the enhanced mobilization and recruitment of BMSCs played an important role in the late phase of IPC.
AB - Objectives: The aim of this study was to investigate whether the mobilization and recruitment of bone marrow stem cells (BMSCs) contribute to cardioprotection in the late phase after ischemic pre-conditioning (IPC). Background: IPC is an innate phenomenon in which brief exposure to sublethal ischemia provides tissue protection from subsequent ischemia/reperfusion (I/R) injury. A delayed cardioprotection also occurs after IPC, but the precise mechanism is unclear. Methods: IPC was created with 4 cycles of 5-min occlusion and reperfusion of the abdominal aorta in mice. Heart I/R injury was induced by occluding the left anterior descending artery for 30 min immediately (early phase) or 24 h (late phase) after IPC. Results: Serum vascular endothelial growth factor and stromal cell-derived factor-1α levels were increased significantly 1 and 3 h after IPC, but CD34+ and CD34+/flk-1+ stem cells in the peripheral blood were increased significantly 12 and 24 h after IPC (p <0.05). Compared with the control treatment, both the early and late phases of IPC protected the heart against I/R injury. However, the recruitment of BMSCs was significantly greater in the heart when I/R injury was induced in late phase than in the early phase of IPC (p <0.01). Interestingly, the blockade of the recruitment of BMSCs significantly attenuated the cardioprotective effect of IPC in the late phase (p <0.01) but did not change in the early phase. Conclusions: Cardioprotection was observed in the early and late phases of IPC; however, the enhanced mobilization and recruitment of BMSCs played an important role in the late phase of IPC.
KW - bone marrow stem cell
KW - ischemic pre-conditioning
KW - late phase
KW - mobilization
KW - recruitment
UR - http://www.scopus.com/inward/record.url?scp=65349114240&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65349114240&partnerID=8YFLogxK
U2 - 10.1016/j.jacc.2009.02.015
DO - 10.1016/j.jacc.2009.02.015
M3 - Article
C2 - 19422991
AN - SCOPUS:65349114240
VL - 53
SP - 1814
EP - 1822
JO - Journal of the American College of Cardiology
JF - Journal of the American College of Cardiology
SN - 0735-1097
IS - 19
ER -