Is carbonic anhydrase activity of photosystem II required for its maximum electron transport rate?

Alexandr V. Shitov, Vasily V. Terentyev, Sergey K. Zharmukhamedov, Margarita V. Rodionova, Mehmet Karacan, Nurcan Karacan, Vyacheslav V. Klimov, Suleyman Allakhverdiev

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

It is known, that the multi-subunit complex of photosystem II (PSII) and some of its single proteins exhibit carbonic anhydrase activity. Previously, we have shown that PSII depletion of HCO3/CO2 as well as the suppression of carbonic anhydrase activity of PSII by a known inhibitor of α‑carbonic anhydrases, acetazolamide (AZM), was accompanied by a decrease of electron transport rate on the PSII donor side. It was concluded that carbonic anhydrase activity was required for maximum photosynthetic activity of PSII but it was not excluded that AZM may have two independent mechanisms of action on PSII: specific and nonspecific. To investigate directly the specific influence of carbonic anhydrase inhibition on the photosynthetic activity in PSII we used another known inhibitor of α‑carbonic anhydrase, trifluoromethanesulfonamide (TFMSA), which molecular structure and physicochemical properties are quite different from those of AZM. In this work, we show for the first time that TFMSA inhibits PSII carbonic anhydrase activity and decreases rates of both the photo-induced changes of chlorophyll fluorescence yield and the photosynthetic oxygen evolution. The inhibitory effect of TFMSA on PSII photosynthetic activity was revealed only in the medium depleted of HCO3/CO2. Addition of exogenous HCO3 or PSII electron donors led to disappearance of the TFMSA inhibitory effect on the electron transport in PSII, indicating that TFMSA inhibition site was located on the PSII donor side. These results show the specificity of TFMSA action on carbonic anhydrase and photosynthetic activities of PSII. In this work, we discuss the necessity of carbonic anhydrase activity for the maximum effectiveness of electron transport on the donor side of PSII.

Original languageEnglish
Pages (from-to)292-299
Number of pages8
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1859
Issue number4
DOIs
Publication statusPublished - Apr 1 2018
Externally publishedYes

Fingerprint

Carbonic Anhydrases
Photosystem II Protein Complex
Electron Transport
Acetazolamide
Carbonic Anhydrase Inhibitors
Chlorophyll
Molecular Structure
Molecular structure

Keywords

  • Carbonic anhydrase
  • Inhibitor
  • Photosystem II
  • Sulfonamide

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Is carbonic anhydrase activity of photosystem II required for its maximum electron transport rate? / Shitov, Alexandr V.; Terentyev, Vasily V.; Zharmukhamedov, Sergey K.; Rodionova, Margarita V.; Karacan, Mehmet; Karacan, Nurcan; Klimov, Vyacheslav V.; Allakhverdiev, Suleyman.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1859, No. 4, 01.04.2018, p. 292-299.

Research output: Contribution to journalArticle

Shitov, AV, Terentyev, VV, Zharmukhamedov, SK, Rodionova, MV, Karacan, M, Karacan, N, Klimov, VV & Allakhverdiev, S 2018, 'Is carbonic anhydrase activity of photosystem II required for its maximum electron transport rate?', Biochimica et Biophysica Acta - Bioenergetics, vol. 1859, no. 4, pp. 292-299. https://doi.org/10.1016/j.bbabio.2018.01.009
Shitov, Alexandr V. ; Terentyev, Vasily V. ; Zharmukhamedov, Sergey K. ; Rodionova, Margarita V. ; Karacan, Mehmet ; Karacan, Nurcan ; Klimov, Vyacheslav V. ; Allakhverdiev, Suleyman. / Is carbonic anhydrase activity of photosystem II required for its maximum electron transport rate?. In: Biochimica et Biophysica Acta - Bioenergetics. 2018 ; Vol. 1859, No. 4. pp. 292-299.
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AU - Rodionova, Margarita V.

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