Irreversible photoinhibition of photosystem II is caused by exposure of Synechocystis cells to strong light for a prolonged period

Suleyman I. Allakhverdiev, Nelly Tsvetkova, Prasanna Mohanty, Balász Szalontai, Yong Moon Byoung, Mónika Debreczeny, Norio Murata

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Irreversible photoinhibition of photosystem II (PSII) occurred when Synechocystis sp. PCC 6803 cells were exposed to very strong light for a prolonged period. When wild-type cells were illuminated at 20°C for 2 h with light at an intensity of 2,500 μmol photons m-2 s-1, the oxygen-evolving activity of PSII was almost entirely and irreversibly lost, whereas the photochemical reaction center in PSII was inactivated only reversibly. The extent of irreversible photoinhibition was enhanced at lower temperatures and by the genetically engineered rigidification of membrane lipids. Western and Northern blotting demonstrated that, after cells had undergone irreversible photoinhibition, the precursor to D1 protein in PSII was synthesized but not processed properly. These observations may suggest that exposure of Synechocystis cells to strong light results in the irreversible photoinhibition of the oxygen-evolving activity of PSII via impairment of the processing of pre-D1 and that this effect of strong light is enhanced by the rigidification of membrane lipids.

Original languageEnglish
Pages (from-to)342-351
Number of pages10
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1708
Issue number3
DOIs
Publication statusPublished - Jul 15 2005

Keywords

  • D1 protein
  • Photodamage
  • Photosystem II
  • Synechocystis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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