Iron-polyethylene glycol impregnation: An attempt to reduce shrinkage of specimens in SEM preparation

Aiji Ohtsuka, T. Murakami

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Glutaraldehyde-fixed HeLa cells were soaked in a mixture of fine cationic iron colloid and polyethylene glycol, immersed in tannic acid solution containing guanidine hydrochloride, and stained with osmic acid. The treated cells showed little shrinkage in the scanning electron microscope even after ethanol dehydration and CO2 critical point drying. On the assumption that every HeLa cell maintained contact with each other, preservation rate was computed as 0.975 ± 0.0033 in linear dimension. Microvilli on the cell surface were well preserved, and few undesirable deposits were noted on the specimen surface. This treatment was also applicable to bulk staining of tissue blocks, such as rat kidneys. The podocyte foot processes and endothelial micropores of the glomerulus were well preserved; the epithelial cells of the Bowman's urinary capsule were not collapsed; the microvilli of the brush border of the proximal convoluted urinary tubule kept their ordinary length (2 μm).

Original languageEnglish
Pages (from-to)177-182
Number of pages6
JournalScanning
Volume10
Issue number5
Publication statusPublished - 1988

Fingerprint

shrinkage
Impregnation
Polyethylene glycols
glycols
polyethylenes
Iron
iron
preparation
Scanning electron microscopy
scanning electron microscopy
Acids
Brushes
cells
Colloids
Dehydration
Rats
Drying
Ethanol
Electron microscopes
Deposits

ASJC Scopus subject areas

  • Instrumentation

Cite this

Iron-polyethylene glycol impregnation : An attempt to reduce shrinkage of specimens in SEM preparation. / Ohtsuka, Aiji; Murakami, T.

In: Scanning, Vol. 10, No. 5, 1988, p. 177-182.

Research output: Contribution to journalArticle

@article{78119d22d01844099166bd326941da86,
title = "Iron-polyethylene glycol impregnation: An attempt to reduce shrinkage of specimens in SEM preparation",
abstract = "Glutaraldehyde-fixed HeLa cells were soaked in a mixture of fine cationic iron colloid and polyethylene glycol, immersed in tannic acid solution containing guanidine hydrochloride, and stained with osmic acid. The treated cells showed little shrinkage in the scanning electron microscope even after ethanol dehydration and CO2 critical point drying. On the assumption that every HeLa cell maintained contact with each other, preservation rate was computed as 0.975 ± 0.0033 in linear dimension. Microvilli on the cell surface were well preserved, and few undesirable deposits were noted on the specimen surface. This treatment was also applicable to bulk staining of tissue blocks, such as rat kidneys. The podocyte foot processes and endothelial micropores of the glomerulus were well preserved; the epithelial cells of the Bowman's urinary capsule were not collapsed; the microvilli of the brush border of the proximal convoluted urinary tubule kept their ordinary length (2 μm).",
author = "Aiji Ohtsuka and T. Murakami",
year = "1988",
language = "English",
volume = "10",
pages = "177--182",
journal = "Scanning",
issn = "0161-0457",
publisher = "John Wiley and Sons Inc.",
number = "5",

}

TY - JOUR

T1 - Iron-polyethylene glycol impregnation

T2 - An attempt to reduce shrinkage of specimens in SEM preparation

AU - Ohtsuka, Aiji

AU - Murakami, T.

PY - 1988

Y1 - 1988

N2 - Glutaraldehyde-fixed HeLa cells were soaked in a mixture of fine cationic iron colloid and polyethylene glycol, immersed in tannic acid solution containing guanidine hydrochloride, and stained with osmic acid. The treated cells showed little shrinkage in the scanning electron microscope even after ethanol dehydration and CO2 critical point drying. On the assumption that every HeLa cell maintained contact with each other, preservation rate was computed as 0.975 ± 0.0033 in linear dimension. Microvilli on the cell surface were well preserved, and few undesirable deposits were noted on the specimen surface. This treatment was also applicable to bulk staining of tissue blocks, such as rat kidneys. The podocyte foot processes and endothelial micropores of the glomerulus were well preserved; the epithelial cells of the Bowman's urinary capsule were not collapsed; the microvilli of the brush border of the proximal convoluted urinary tubule kept their ordinary length (2 μm).

AB - Glutaraldehyde-fixed HeLa cells were soaked in a mixture of fine cationic iron colloid and polyethylene glycol, immersed in tannic acid solution containing guanidine hydrochloride, and stained with osmic acid. The treated cells showed little shrinkage in the scanning electron microscope even after ethanol dehydration and CO2 critical point drying. On the assumption that every HeLa cell maintained contact with each other, preservation rate was computed as 0.975 ± 0.0033 in linear dimension. Microvilli on the cell surface were well preserved, and few undesirable deposits were noted on the specimen surface. This treatment was also applicable to bulk staining of tissue blocks, such as rat kidneys. The podocyte foot processes and endothelial micropores of the glomerulus were well preserved; the epithelial cells of the Bowman's urinary capsule were not collapsed; the microvilli of the brush border of the proximal convoluted urinary tubule kept their ordinary length (2 μm).

UR - http://www.scopus.com/inward/record.url?scp=0024265120&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024265120&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0024265120

VL - 10

SP - 177

EP - 182

JO - Scanning

JF - Scanning

SN - 0161-0457

IS - 5

ER -