Irnmunohistochemical study of glutathione S-transferase (GST-π) on hepatocellular carcinoma

Keiki Ogino; Isao Sakaida; Shinji Oka; Hujio Murakami; Keisuke Hino; Kazutoshi Sanuki; Yasushi Shingai; Hiroshi Nawata; Yuji Kado; Mitsuru Yasunaga; Takaro Ezaki; Keijiro Ando; Kiwamu Okita; Tadayoshi Takemoto

doi:10.2957/kanzo.27.1439

Irnmunohistochemical study of glutathione S-transferase (GST-π) on hepatocellular carcinoma

Keiki Ogino, Isao Sakaida, Shinji Oka, Hujio Murakami, Keisuke Hino, Kazutoshi Sanuki, Yasushi Shingai, Hiroshi Nawata, Yuji Kado, Mitsuru Yasunaga, Takaro Ezaki, Keijiro Ando, Kiwamu Okita, Tadayoshi Takemoto

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Anionic form of glutathione S-transferase (Gst-π)was purified in apparent homogenity from human placenta. The molecular weight of GST-π was 21,000 by SDS-polyacrylamide gel electrophoresis and isoelectric point was 4.5 by analytical electrophocussing gel electrophoresis. According to Ouchtrlony’s method, antigenicity of GSY-π was identical with anionic GST from hepatocellular carcinoma and kidney. Immunohistochemical localization of GST-n in 30 cases of hepatocellular carcinoma revealed that positive staining was shown in 50% cases compared to Edmondson’s grade I or II. It was interesting that hepatocytes around cancer tissue, not found out atypism, were stained intensively sometimes. Therefore, it is suggested that Gst-πmay be tumor marker for hepatocellular carcinoma. However, the investigation of GST-π rich hepatocytes around cancer tissue is necessary in connection with pre-cancerous lesion.

Original language	English
Pages (from-to)	1439-1443
Number of pages	5
Journal	Kanzo
Volume	27
Issue number	10
DOIs	https://doi.org/10.2957/kanzo.27.1439
Publication status	Published - 1986

ASJC Scopus subject areas

Hepatology

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Irnmunohistochemical study of glutathione S-transferase (GST-π) on hepatocellular carcinoma. / Ogino, Keiki; Sakaida, Isao; Oka, Shinji; Murakami, Hujio; Hino, Keisuke; Sanuki, Kazutoshi; Shingai, Yasushi; Nawata, Hiroshi; Kado, Yuji; Yasunaga, Mitsuru; Ezaki, Takaro; Ando, Keijiro; Okita, Kiwamu; Takemoto, Tadayoshi.

In: Kanzo, Vol. 27, No. 10, 1986, p. 1439-1443.

Research output: Contribution to journal › Article › peer-review

Ogino, Keiki ; Sakaida, Isao ; Oka, Shinji ; Murakami, Hujio ; Hino, Keisuke ; Sanuki, Kazutoshi ; Shingai, Yasushi ; Nawata, Hiroshi ; Kado, Yuji ; Yasunaga, Mitsuru ; Ezaki, Takaro ; Ando, Keijiro ; Okita, Kiwamu ; Takemoto, Tadayoshi. / Irnmunohistochemical study of glutathione S-transferase (GST-π) on hepatocellular carcinoma. In: Kanzo. 1986 ; Vol. 27, No. 10. pp. 1439-1443.

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title = "Irnmunohistochemical study of glutathione S-transferase (GST-π) on hepatocellular carcinoma",

abstract = "Anionic form of glutathione S-transferase (Gst-π)was purified in apparent homogenity from human placenta. The molecular weight of GST-π was 21,000 by SDS-polyacrylamide gel electrophoresis and isoelectric point was 4.5 by analytical electrophocussing gel electrophoresis. According to Ouchtrlony{\textquoteright}s method, antigenicity of GSY-π was identical with anionic GST from hepatocellular carcinoma and kidney. Immunohistochemical localization of GST-n in 30 cases of hepatocellular carcinoma revealed that positive staining was shown in 50% cases compared to Edmondson{\textquoteright}s grade I or II. It was interesting that hepatocytes around cancer tissue, not found out atypism, were stained intensively sometimes. Therefore, it is suggested that Gst-πmay be tumor marker for hepatocellular carcinoma. However, the investigation of GST-π rich hepatocytes around cancer tissue is necessary in connection with pre-cancerous lesion.",

author = "Keiki Ogino and Isao Sakaida and Shinji Oka and Hujio Murakami and Keisuke Hino and Kazutoshi Sanuki and Yasushi Shingai and Hiroshi Nawata and Yuji Kado and Mitsuru Yasunaga and Takaro Ezaki and Keijiro Ando and Kiwamu Okita and Tadayoshi Takemoto",

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AU - Hino, Keisuke

AU - Sanuki, Kazutoshi

AU - Shingai, Yasushi

AU - Nawata, Hiroshi

AU - Kado, Yuji

AU - Yasunaga, Mitsuru

AU - Ezaki, Takaro

AU - Ando, Keijiro

AU - Okita, Kiwamu

AU - Takemoto, Tadayoshi

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