Abstract
Anionic form of glutathione S-transferase (Gst-π)was purified in apparent homogenity from human placenta. The molecular weight of GST-π was 21,000 by SDS-polyacrylamide gel electrophoresis and isoelectric point was 4.5 by analytical electrophocussing gel electrophoresis. According to Ouchtrlony’s method, antigenicity of GSY-π was identical with anionic GST from hepatocellular carcinoma and kidney. Immunohistochemical localization of GST-n in 30 cases of hepatocellular carcinoma revealed that positive staining was shown in 50% cases compared to Edmondson’s grade I or II. It was interesting that hepatocytes around cancer tissue, not found out atypism, were stained intensively sometimes. Therefore, it is suggested that Gst-πmay be tumor marker for hepatocellular carcinoma. However, the investigation of GST-π rich hepatocytes around cancer tissue is necessary in connection with pre-cancerous lesion.
| Original language | English |
|---|---|
| Pages (from-to) | 1439-1443 |
| Number of pages | 5 |
| Journal | Kanzo |
| Volume | 27 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 1986 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Hepatology
Cite this
Irnmunohistochemical study of glutathione S-transferase (GST-π) on hepatocellular carcinoma. / Ogino, Keiki; Sakaida, Isao; Oka, Shinji; Murakami, Hujio; Hino, Keisuke; Sanuki, Kazutoshi; Shingai, Yasushi; Nawata, Hiroshi; Kado, Yuji; Yasunaga, Mitsuru; Ezaki, Takaro; Ando, Keijiro; Okita, Kiwamu; Takemoto, Tadayoshi.
In: Kanzo, Vol. 27, No. 10, 1986, p. 1439-1443.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Irnmunohistochemical study of glutathione S-transferase (GST-π) on hepatocellular carcinoma
AU - Ogino, Keiki
AU - Sakaida, Isao
AU - Oka, Shinji
AU - Murakami, Hujio
AU - Hino, Keisuke
AU - Sanuki, Kazutoshi
AU - Shingai, Yasushi
AU - Nawata, Hiroshi
AU - Kado, Yuji
AU - Yasunaga, Mitsuru
AU - Ezaki, Takaro
AU - Ando, Keijiro
AU - Okita, Kiwamu
AU - Takemoto, Tadayoshi
PY - 1986
Y1 - 1986
N2 - Anionic form of glutathione S-transferase (Gst-π)was purified in apparent homogenity from human placenta. The molecular weight of GST-π was 21,000 by SDS-polyacrylamide gel electrophoresis and isoelectric point was 4.5 by analytical electrophocussing gel electrophoresis. According to Ouchtrlony’s method, antigenicity of GSY-π was identical with anionic GST from hepatocellular carcinoma and kidney. Immunohistochemical localization of GST-n in 30 cases of hepatocellular carcinoma revealed that positive staining was shown in 50% cases compared to Edmondson’s grade I or II. It was interesting that hepatocytes around cancer tissue, not found out atypism, were stained intensively sometimes. Therefore, it is suggested that Gst-πmay be tumor marker for hepatocellular carcinoma. However, the investigation of GST-π rich hepatocytes around cancer tissue is necessary in connection with pre-cancerous lesion.
AB - Anionic form of glutathione S-transferase (Gst-π)was purified in apparent homogenity from human placenta. The molecular weight of GST-π was 21,000 by SDS-polyacrylamide gel electrophoresis and isoelectric point was 4.5 by analytical electrophocussing gel electrophoresis. According to Ouchtrlony’s method, antigenicity of GSY-π was identical with anionic GST from hepatocellular carcinoma and kidney. Immunohistochemical localization of GST-n in 30 cases of hepatocellular carcinoma revealed that positive staining was shown in 50% cases compared to Edmondson’s grade I or II. It was interesting that hepatocytes around cancer tissue, not found out atypism, were stained intensively sometimes. Therefore, it is suggested that Gst-πmay be tumor marker for hepatocellular carcinoma. However, the investigation of GST-π rich hepatocytes around cancer tissue is necessary in connection with pre-cancerous lesion.
UR - http://www.scopus.com/inward/record.url?scp=85004500516&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85004500516&partnerID=8YFLogxK
U2 - 10.2957/kanzo.27.1439
DO - 10.2957/kanzo.27.1439
M3 - Article
AN - SCOPUS:85004500516
VL - 27
SP - 1439
EP - 1443
JO - Acta Hepatologica Japonica
JF - Acta Hepatologica Japonica
SN - 0451-4203
IS - 10
ER -