Iris movement mediates vascular apoptosis during rat pupillary membrane regression

Yuki Morizane, Satoshi Mohri, Jun Kosaka, Shigenobu Toné, Takahiko Kiyooka, Takehiro Miyasaka, Juichiro Shimizu, Yasuo Ogasawara, Fumio Shiraga, Yohsuke Minatogawa, Junzo Sasaki, Hiroshi Ohtsuki, Fumihiko Kajiya

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In the course of mammalian lens development, a transient capillary meshwork known as the pupillary membrane (PM) forms, which is located at the pupil area; the PM nourishes the anterior surface of the lens and then regresses to make the optical path clear. Although the involvement of apoptotic process has been reported in the PM regression, the initiating factor remains unknown. We initially found that regression of the PM coincided with the development of iris motility, and iris movement caused cessation and resumption of blood flow within the PM. Therefore, we investigated whether the development of the iris's ability to constrict and dilate functions as an essential signal that induces apoptosis in the PM. Continuous inhibition of iris movement with mydriatic agents from postnatal day 7 to day 12 suppressed apoptosis of the PM and migration of macrophage toward the PM, and resulted in the persistence of PM in rats. The distribution of apoptotic cells in the regressing PM was diffuse and showed no apparent localization. These results indicated that iris movement induced regression of the PM by changing the blood flow within it. This study suggests the importance of the physiological interactions between tissues - in this case, the iris and the PM - as a signal to advance vascular regression during organ development, and defines a novel function of the iris during ocular development in addition to the well-known function, that is, optimization of light transmission into the eye.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume290
Issue number3
DOIs
Publication statusPublished - Mar 2006

Fingerprint

Iris
Blood Vessels
Apoptosis
Membranes
Lenses
Mydriatics
Pupil
Macrophages
Light

Keywords

  • Microcirculation
  • Miosis
  • Mydriasis
  • Ocular development

ASJC Scopus subject areas

  • Physiology

Cite this

Iris movement mediates vascular apoptosis during rat pupillary membrane regression. / Morizane, Yuki; Mohri, Satoshi; Kosaka, Jun; Toné, Shigenobu; Kiyooka, Takahiko; Miyasaka, Takehiro; Shimizu, Juichiro; Ogasawara, Yasuo; Shiraga, Fumio; Minatogawa, Yohsuke; Sasaki, Junzo; Ohtsuki, Hiroshi; Kajiya, Fumihiko.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 290, No. 3, 03.2006.

Research output: Contribution to journalArticle

Morizane, Y, Mohri, S, Kosaka, J, Toné, S, Kiyooka, T, Miyasaka, T, Shimizu, J, Ogasawara, Y, Shiraga, F, Minatogawa, Y, Sasaki, J, Ohtsuki, H & Kajiya, F 2006, 'Iris movement mediates vascular apoptosis during rat pupillary membrane regression', American Journal of Physiology - Regulatory Integrative and Comparative Physiology, vol. 290, no. 3. https://doi.org/10.1152/ajpregu.00602.2005
Morizane, Yuki ; Mohri, Satoshi ; Kosaka, Jun ; Toné, Shigenobu ; Kiyooka, Takahiko ; Miyasaka, Takehiro ; Shimizu, Juichiro ; Ogasawara, Yasuo ; Shiraga, Fumio ; Minatogawa, Yohsuke ; Sasaki, Junzo ; Ohtsuki, Hiroshi ; Kajiya, Fumihiko. / Iris movement mediates vascular apoptosis during rat pupillary membrane regression. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2006 ; Vol. 290, No. 3.
@article{4f21e74194a24916bfa6ecdc3e52f325,
title = "Iris movement mediates vascular apoptosis during rat pupillary membrane regression",
abstract = "In the course of mammalian lens development, a transient capillary meshwork known as the pupillary membrane (PM) forms, which is located at the pupil area; the PM nourishes the anterior surface of the lens and then regresses to make the optical path clear. Although the involvement of apoptotic process has been reported in the PM regression, the initiating factor remains unknown. We initially found that regression of the PM coincided with the development of iris motility, and iris movement caused cessation and resumption of blood flow within the PM. Therefore, we investigated whether the development of the iris's ability to constrict and dilate functions as an essential signal that induces apoptosis in the PM. Continuous inhibition of iris movement with mydriatic agents from postnatal day 7 to day 12 suppressed apoptosis of the PM and migration of macrophage toward the PM, and resulted in the persistence of PM in rats. The distribution of apoptotic cells in the regressing PM was diffuse and showed no apparent localization. These results indicated that iris movement induced regression of the PM by changing the blood flow within it. This study suggests the importance of the physiological interactions between tissues - in this case, the iris and the PM - as a signal to advance vascular regression during organ development, and defines a novel function of the iris during ocular development in addition to the well-known function, that is, optimization of light transmission into the eye.",
keywords = "Microcirculation, Miosis, Mydriasis, Ocular development",
author = "Yuki Morizane and Satoshi Mohri and Jun Kosaka and Shigenobu Ton{\'e} and Takahiko Kiyooka and Takehiro Miyasaka and Juichiro Shimizu and Yasuo Ogasawara and Fumio Shiraga and Yohsuke Minatogawa and Junzo Sasaki and Hiroshi Ohtsuki and Fumihiko Kajiya",
year = "2006",
month = "3",
doi = "10.1152/ajpregu.00602.2005",
language = "English",
volume = "290",
journal = "American Journal of Physiology - Regulatory Integrative and Comparative Physiology",
issn = "0363-6119",
publisher = "American Physiological Society",
number = "3",

}

TY - JOUR

T1 - Iris movement mediates vascular apoptosis during rat pupillary membrane regression

AU - Morizane, Yuki

AU - Mohri, Satoshi

AU - Kosaka, Jun

AU - Toné, Shigenobu

AU - Kiyooka, Takahiko

AU - Miyasaka, Takehiro

AU - Shimizu, Juichiro

AU - Ogasawara, Yasuo

AU - Shiraga, Fumio

AU - Minatogawa, Yohsuke

AU - Sasaki, Junzo

AU - Ohtsuki, Hiroshi

AU - Kajiya, Fumihiko

PY - 2006/3

Y1 - 2006/3

N2 - In the course of mammalian lens development, a transient capillary meshwork known as the pupillary membrane (PM) forms, which is located at the pupil area; the PM nourishes the anterior surface of the lens and then regresses to make the optical path clear. Although the involvement of apoptotic process has been reported in the PM regression, the initiating factor remains unknown. We initially found that regression of the PM coincided with the development of iris motility, and iris movement caused cessation and resumption of blood flow within the PM. Therefore, we investigated whether the development of the iris's ability to constrict and dilate functions as an essential signal that induces apoptosis in the PM. Continuous inhibition of iris movement with mydriatic agents from postnatal day 7 to day 12 suppressed apoptosis of the PM and migration of macrophage toward the PM, and resulted in the persistence of PM in rats. The distribution of apoptotic cells in the regressing PM was diffuse and showed no apparent localization. These results indicated that iris movement induced regression of the PM by changing the blood flow within it. This study suggests the importance of the physiological interactions between tissues - in this case, the iris and the PM - as a signal to advance vascular regression during organ development, and defines a novel function of the iris during ocular development in addition to the well-known function, that is, optimization of light transmission into the eye.

AB - In the course of mammalian lens development, a transient capillary meshwork known as the pupillary membrane (PM) forms, which is located at the pupil area; the PM nourishes the anterior surface of the lens and then regresses to make the optical path clear. Although the involvement of apoptotic process has been reported in the PM regression, the initiating factor remains unknown. We initially found that regression of the PM coincided with the development of iris motility, and iris movement caused cessation and resumption of blood flow within the PM. Therefore, we investigated whether the development of the iris's ability to constrict and dilate functions as an essential signal that induces apoptosis in the PM. Continuous inhibition of iris movement with mydriatic agents from postnatal day 7 to day 12 suppressed apoptosis of the PM and migration of macrophage toward the PM, and resulted in the persistence of PM in rats. The distribution of apoptotic cells in the regressing PM was diffuse and showed no apparent localization. These results indicated that iris movement induced regression of the PM by changing the blood flow within it. This study suggests the importance of the physiological interactions between tissues - in this case, the iris and the PM - as a signal to advance vascular regression during organ development, and defines a novel function of the iris during ocular development in addition to the well-known function, that is, optimization of light transmission into the eye.

KW - Microcirculation

KW - Miosis

KW - Mydriasis

KW - Ocular development

UR - http://www.scopus.com/inward/record.url?scp=33645422960&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645422960&partnerID=8YFLogxK

U2 - 10.1152/ajpregu.00602.2005

DO - 10.1152/ajpregu.00602.2005

M3 - Article

C2 - 16223846

AN - SCOPUS:33645422960

VL - 290

JO - American Journal of Physiology - Regulatory Integrative and Comparative Physiology

JF - American Journal of Physiology - Regulatory Integrative and Comparative Physiology

SN - 0363-6119

IS - 3

ER -