Ionotropic glutamate receptors expressed in human retinoblastoma Y79 cells

Michiko Takeda, Megumi Haga, Hiroshi Yamada, Mika Kinoshita, Masato Otsuka, Seiji Tsuboi, Yoshinori Moriyama

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Mammalian retinal photoreceptors and pinealocytes have common characteristic that they secrete melatonin and L-glutamate as chemical transmitters. Although pinealocytes express glutamate receptors and receive glutamate signals, whether or not photoreceptors express glutamate receptors is unknown. Here, we investigated the expression of the glutamate receptors in cultured Y79 clonal human retinoblastoma cells, as model systems of photoreceptors. Reverse transcription-polymerase chain reaction (RT-PCR) analysis indicated that GluR1, GluR5, GluR7, EAA2, NR1, NR2A and NR2D mRNAs were present in the cultured cells. Northern analysis confirmed the presence of GluR7, EAA2, NR1, NR2A and NR2D mRNAs, while other mRNAs were under the detection limit. Addition of (RS)-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid or kainate increases intracellular (Ca2+) in Fura-2 loaded cells, which is blocked by 6-cyano-7-nitroquinoxaline-2,3-dione. N-methyl-D-aspartate also increases intracellular (Ca2+). These results demonstrated the presence of functional ionotropic receptors in Y79 cells. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)97-100
Number of pages4
JournalNeuroscience Letters
Volume294
Issue number2
DOIs
Publication statusPublished - Nov 17 2000

Fingerprint

Ionotropic Glutamate Receptors
Retinoblastoma
Glutamate Receptors
Messenger RNA
Glutamic Acid
6-Cyano-7-nitroquinoxaline-2,3-dione
Vertebrate Photoreceptor Cells
Fura-2
Kainic Acid
N-Methylaspartate
Melatonin
Reverse Transcription
Limit of Detection
Cultured Cells
Polymerase Chain Reaction
Acids

Keywords

  • Glutamate
  • Glutamate receptor
  • Human retinoblastoma
  • Melatonin
  • Photoreceptor
  • Pineal gland
  • Retina
  • Y79

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Takeda, M., Haga, M., Yamada, H., Kinoshita, M., Otsuka, M., Tsuboi, S., & Moriyama, Y. (2000). Ionotropic glutamate receptors expressed in human retinoblastoma Y79 cells. Neuroscience Letters, 294(2), 97-100. https://doi.org/10.1016/S0304-3940(00)01546-9

Ionotropic glutamate receptors expressed in human retinoblastoma Y79 cells. / Takeda, Michiko; Haga, Megumi; Yamada, Hiroshi; Kinoshita, Mika; Otsuka, Masato; Tsuboi, Seiji; Moriyama, Yoshinori.

In: Neuroscience Letters, Vol. 294, No. 2, 17.11.2000, p. 97-100.

Research output: Contribution to journalArticle

Takeda, M, Haga, M, Yamada, H, Kinoshita, M, Otsuka, M, Tsuboi, S & Moriyama, Y 2000, 'Ionotropic glutamate receptors expressed in human retinoblastoma Y79 cells', Neuroscience Letters, vol. 294, no. 2, pp. 97-100. https://doi.org/10.1016/S0304-3940(00)01546-9
Takeda, Michiko ; Haga, Megumi ; Yamada, Hiroshi ; Kinoshita, Mika ; Otsuka, Masato ; Tsuboi, Seiji ; Moriyama, Yoshinori. / Ionotropic glutamate receptors expressed in human retinoblastoma Y79 cells. In: Neuroscience Letters. 2000 ; Vol. 294, No. 2. pp. 97-100.
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