Abstract
Mammalian retinal photoreceptors and pinealocytes have common characteristic that they secrete melatonin and L-glutamate as chemical transmitters. Although pinealocytes express glutamate receptors and receive glutamate signals, whether or not photoreceptors express glutamate receptors is unknown. Here, we investigated the expression of the glutamate receptors in cultured Y79 clonal human retinoblastoma cells, as model systems of photoreceptors. Reverse transcription-polymerase chain reaction (RT-PCR) analysis indicated that GluR1, GluR5, GluR7, EAA2, NR1, NR2A and NR2D mRNAs were present in the cultured cells. Northern analysis confirmed the presence of GluR7, EAA2, NR1, NR2A and NR2D mRNAs, while other mRNAs were under the detection limit. Addition of (RS)-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid or kainate increases intracellular (Ca2+) in Fura-2 loaded cells, which is blocked by 6-cyano-7-nitroquinoxaline-2,3-dione. N-methyl-D-aspartate also increases intracellular (Ca2+). These results demonstrated the presence of functional ionotropic receptors in Y79 cells. Copyright (C) 2000 Elsevier Science Ireland Ltd.
| Original language | English |
|---|---|
| Pages (from-to) | 97-100 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 294 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Nov 17 2000 |
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Keywords
- Glutamate
- Glutamate receptor
- Human retinoblastoma
- Melatonin
- Photoreceptor
- Pineal gland
- Retina
- Y79
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Ionotropic glutamate receptors expressed in human retinoblastoma Y79 cells. / Takeda, Michiko; Haga, Megumi; Yamada, Hiroshi; Kinoshita, Mika; Otsuka, Masato; Tsuboi, Seiji; Moriyama, Yoshinori.
In: Neuroscience Letters, Vol. 294, No. 2, 17.11.2000, p. 97-100.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ionotropic glutamate receptors expressed in human retinoblastoma Y79 cells
AU - Takeda, Michiko
AU - Haga, Megumi
AU - Yamada, Hiroshi
AU - Kinoshita, Mika
AU - Otsuka, Masato
AU - Tsuboi, Seiji
AU - Moriyama, Yoshinori
PY - 2000/11/17
Y1 - 2000/11/17
N2 - Mammalian retinal photoreceptors and pinealocytes have common characteristic that they secrete melatonin and L-glutamate as chemical transmitters. Although pinealocytes express glutamate receptors and receive glutamate signals, whether or not photoreceptors express glutamate receptors is unknown. Here, we investigated the expression of the glutamate receptors in cultured Y79 clonal human retinoblastoma cells, as model systems of photoreceptors. Reverse transcription-polymerase chain reaction (RT-PCR) analysis indicated that GluR1, GluR5, GluR7, EAA2, NR1, NR2A and NR2D mRNAs were present in the cultured cells. Northern analysis confirmed the presence of GluR7, EAA2, NR1, NR2A and NR2D mRNAs, while other mRNAs were under the detection limit. Addition of (RS)-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid or kainate increases intracellular (Ca2+) in Fura-2 loaded cells, which is blocked by 6-cyano-7-nitroquinoxaline-2,3-dione. N-methyl-D-aspartate also increases intracellular (Ca2+). These results demonstrated the presence of functional ionotropic receptors in Y79 cells. Copyright (C) 2000 Elsevier Science Ireland Ltd.
AB - Mammalian retinal photoreceptors and pinealocytes have common characteristic that they secrete melatonin and L-glutamate as chemical transmitters. Although pinealocytes express glutamate receptors and receive glutamate signals, whether or not photoreceptors express glutamate receptors is unknown. Here, we investigated the expression of the glutamate receptors in cultured Y79 clonal human retinoblastoma cells, as model systems of photoreceptors. Reverse transcription-polymerase chain reaction (RT-PCR) analysis indicated that GluR1, GluR5, GluR7, EAA2, NR1, NR2A and NR2D mRNAs were present in the cultured cells. Northern analysis confirmed the presence of GluR7, EAA2, NR1, NR2A and NR2D mRNAs, while other mRNAs were under the detection limit. Addition of (RS)-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid or kainate increases intracellular (Ca2+) in Fura-2 loaded cells, which is blocked by 6-cyano-7-nitroquinoxaline-2,3-dione. N-methyl-D-aspartate also increases intracellular (Ca2+). These results demonstrated the presence of functional ionotropic receptors in Y79 cells. Copyright (C) 2000 Elsevier Science Ireland Ltd.
KW - Glutamate
KW - Glutamate receptor
KW - Human retinoblastoma
KW - Melatonin
KW - Photoreceptor
KW - Pineal gland
KW - Retina
KW - Y79
UR - http://www.scopus.com/inward/record.url?scp=0034681047&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034681047&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(00)01546-9
DO - 10.1016/S0304-3940(00)01546-9
M3 - Article
VL - 294
SP - 97
EP - 100
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 2
ER -