Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp.

Suleyman I. Allakhverdiev, Atsushi Sakamoto, Yoshitaka Nishiyama, Masami Inaba, Norio Murata

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Abstract

We report here that osmotic effects and ionic effects are both involved in the NaCl-induced inactivation of the photosynthetic machinery in the cyanobacterium Synechococcus sp. PCC 7942. Incubation of the cyanobacterial cells in 0.5 M NaCl induced a rapid and reversible decline and subsequent slow and irreversible loss of the oxygen-evolving activity of photosystem (PS) II and the electron transport activity of PSI. An Na+-channel blocker protected both PSII and PSI against the slow, but not the rapid, inactivation. The rapid decline resembled the effect of 1.0 M sorbitol. The presence of both an Na+-channel blocker and a water-channel blocker protected PSI and PSII against the short- and long-term effects of NaCl. Salt stress also decreased cytoplasmic volume and this effect was enhanced by the Na+-channel blocker. Our observations suggested that NaCl had both osmotic and ionic effects. The osmotic effect decreased the amount of water in the cytosol, rapidly increasing the intracellular concentration of salts. The ionic effect was caused by an influx of Na+ ions through potassium/Na+ channels that also increased concentrations of salts in the cytosol and irreversibly inactivated PSI and PSII.

Original languageEnglish
Pages (from-to)1047-1056
Number of pages10
JournalPlant physiology
Volume123
Issue number3
DOIs
Publication statusPublished - Jul 2000

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ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Allakhverdiev, S. I., Sakamoto, A., Nishiyama, Y., Inaba, M., & Murata, N. (2000). Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp. Plant physiology, 123(3), 1047-1056. https://doi.org/10.1104/pp.123.3.1047