Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp.

Suleyman Allakhverdiev, Atsushi Sakamoto, Yoshitaka Nishiyama, Masami Inaba, Norio Murata

Research output: Contribution to journalArticle

363 Citations (Scopus)

Abstract

We report here that osmotic effects and ionic effects are both involved in the NaCl-induced inactivation of the photosynthetic machinery in the cyanobacterium Synechococcus sp. PCC 7942. Incubation of the cyanobacterial cells in 0.5 M NaCl induced a rapid and reversible decline and subsequent slow and irreversible loss of the oxygen-evolving activity of photosystem (PS) II and the electron transport activity of PSI. An Na+-channel blocker protected both PSII and PSI against the slow, but not the rapid, inactivation. The rapid decline resembled the effect of 1.0 M sorbitol. The presence of both an Na+-channel blocker and a water-channel blocker protected PSI and PSII against the short- and long-term effects of NaCl. Salt stress also decreased cytoplasmic volume and this effect was enhanced by the Na+-channel blocker. Our observations suggested that NaCl had both osmotic and ionic effects. The osmotic effect decreased the amount of water in the cytosol, rapidly increasing the intracellular concentration of salts. The ionic effect was caused by an influx of Na+ ions through potassium/Na+ channels that also increased concentrations of salts in the cytosol and irreversibly inactivated PSI and PSII.

Original languageEnglish
Pages (from-to)1047-1056
Number of pages10
JournalPlant Physiology
Volume123
Issue number3
DOIs
Publication statusPublished - Jan 1 2000
Externally publishedYes

Fingerprint

Synechococcus
Photosystem I Protein Complex
Photosystem II Protein Complex
photosystem I
salt concentration
cytosol
photosystem II
inactivation
Salts
Synechococcus sp. PCC 7942
Cytosol
sorbitol
electron transfer
salt stress
long term effects
Cyanobacteria
Aquaporins
Sorbitol
water
Potassium Channels

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Allakhverdiev, S., Sakamoto, A., Nishiyama, Y., Inaba, M., & Murata, N. (2000). Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp. Plant Physiology, 123(3), 1047-1056. https://doi.org/10.1104/pp.123.3.1047

Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp. / Allakhverdiev, Suleyman; Sakamoto, Atsushi; Nishiyama, Yoshitaka; Inaba, Masami; Murata, Norio.

In: Plant Physiology, Vol. 123, No. 3, 01.01.2000, p. 1047-1056.

Research output: Contribution to journalArticle

Allakhverdiev, S, Sakamoto, A, Nishiyama, Y, Inaba, M & Murata, N 2000, 'Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp.', Plant Physiology, vol. 123, no. 3, pp. 1047-1056. https://doi.org/10.1104/pp.123.3.1047
Allakhverdiev, Suleyman ; Sakamoto, Atsushi ; Nishiyama, Yoshitaka ; Inaba, Masami ; Murata, Norio. / Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp. In: Plant Physiology. 2000 ; Vol. 123, No. 3. pp. 1047-1056.
@article{a659ae4e6d1e4009ad70dcc2655f7f4a,
title = "Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp.",
abstract = "We report here that osmotic effects and ionic effects are both involved in the NaCl-induced inactivation of the photosynthetic machinery in the cyanobacterium Synechococcus sp. PCC 7942. Incubation of the cyanobacterial cells in 0.5 M NaCl induced a rapid and reversible decline and subsequent slow and irreversible loss of the oxygen-evolving activity of photosystem (PS) II and the electron transport activity of PSI. An Na+-channel blocker protected both PSII and PSI against the slow, but not the rapid, inactivation. The rapid decline resembled the effect of 1.0 M sorbitol. The presence of both an Na+-channel blocker and a water-channel blocker protected PSI and PSII against the short- and long-term effects of NaCl. Salt stress also decreased cytoplasmic volume and this effect was enhanced by the Na+-channel blocker. Our observations suggested that NaCl had both osmotic and ionic effects. The osmotic effect decreased the amount of water in the cytosol, rapidly increasing the intracellular concentration of salts. The ionic effect was caused by an influx of Na+ ions through potassium/Na+ channels that also increased concentrations of salts in the cytosol and irreversibly inactivated PSI and PSII.",
author = "Suleyman Allakhverdiev and Atsushi Sakamoto and Yoshitaka Nishiyama and Masami Inaba and Norio Murata",
year = "2000",
month = "1",
day = "1",
doi = "10.1104/pp.123.3.1047",
language = "English",
volume = "123",
pages = "1047--1056",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "3",

}

TY - JOUR

T1 - Ionic and osmotic effects of NaCl-induced inactivation of photosystems I and II in Synechococcus sp.

AU - Allakhverdiev, Suleyman

AU - Sakamoto, Atsushi

AU - Nishiyama, Yoshitaka

AU - Inaba, Masami

AU - Murata, Norio

PY - 2000/1/1

Y1 - 2000/1/1

N2 - We report here that osmotic effects and ionic effects are both involved in the NaCl-induced inactivation of the photosynthetic machinery in the cyanobacterium Synechococcus sp. PCC 7942. Incubation of the cyanobacterial cells in 0.5 M NaCl induced a rapid and reversible decline and subsequent slow and irreversible loss of the oxygen-evolving activity of photosystem (PS) II and the electron transport activity of PSI. An Na+-channel blocker protected both PSII and PSI against the slow, but not the rapid, inactivation. The rapid decline resembled the effect of 1.0 M sorbitol. The presence of both an Na+-channel blocker and a water-channel blocker protected PSI and PSII against the short- and long-term effects of NaCl. Salt stress also decreased cytoplasmic volume and this effect was enhanced by the Na+-channel blocker. Our observations suggested that NaCl had both osmotic and ionic effects. The osmotic effect decreased the amount of water in the cytosol, rapidly increasing the intracellular concentration of salts. The ionic effect was caused by an influx of Na+ ions through potassium/Na+ channels that also increased concentrations of salts in the cytosol and irreversibly inactivated PSI and PSII.

AB - We report here that osmotic effects and ionic effects are both involved in the NaCl-induced inactivation of the photosynthetic machinery in the cyanobacterium Synechococcus sp. PCC 7942. Incubation of the cyanobacterial cells in 0.5 M NaCl induced a rapid and reversible decline and subsequent slow and irreversible loss of the oxygen-evolving activity of photosystem (PS) II and the electron transport activity of PSI. An Na+-channel blocker protected both PSII and PSI against the slow, but not the rapid, inactivation. The rapid decline resembled the effect of 1.0 M sorbitol. The presence of both an Na+-channel blocker and a water-channel blocker protected PSI and PSII against the short- and long-term effects of NaCl. Salt stress also decreased cytoplasmic volume and this effect was enhanced by the Na+-channel blocker. Our observations suggested that NaCl had both osmotic and ionic effects. The osmotic effect decreased the amount of water in the cytosol, rapidly increasing the intracellular concentration of salts. The ionic effect was caused by an influx of Na+ ions through potassium/Na+ channels that also increased concentrations of salts in the cytosol and irreversibly inactivated PSI and PSII.

UR - http://www.scopus.com/inward/record.url?scp=0033941856&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033941856&partnerID=8YFLogxK

U2 - 10.1104/pp.123.3.1047

DO - 10.1104/pp.123.3.1047

M3 - Article

C2 - 10889254

AN - SCOPUS:0033941856

VL - 123

SP - 1047

EP - 1056

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 3

ER -