Ion gradients in xylem exudate and guttation fluid related to tissue ion levels along primary leaves of barley

Makiko Nagai, Miwa Ohnishi, Takeo Uehara, Mutsumi Yamagami, Eiko Miura, Mai Kamakura, Akira Kitamura, Shu Ichi Sakaguchi, Wataru Sakamoto, Teruo Shimmen, Hidehiro Fukaki, Robert J. Reid, Akio Furukawa, Tetsuro Mimura

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The concentration of ions in plant cells and tissues is an essential factor in determining physiological function. In the present study, we established that concentration gradients of mobile ions exist in both xylem exudates and tissues within a barley (Hordeum vulgare) primary leaf. For K+ and NO3-, ion concentrations generally decreased from the leaf base to the tip in both xylem exudates and tissues. Ion gradients were also found for Pi and Cl- in the xylem. The hydathode strongly absorbed Pi and re-translocated it to the rest of the plant, whereas Cl- was extruded. The ion concentration gradients developed early during leaf growth, increased as the tissue aged and remained under both high and low transpiration conditions. Measurement of the expression profiles of Pi, K+ and NO3- transporters along the longitudinal axis of the leaf revealed that some transporters are more expressed at the hydathode, but for most transporters, there was no significant variation along the leaf. The mechanisms by which longitudinal ion gradients develop in leaves and their physiological functions are discussed.

Original languageEnglish
Pages (from-to)1826-1837
Number of pages12
JournalPlant, Cell and Environment
Volume36
Issue number10
DOIs
Publication statusPublished - Oct 2013

Fingerprint

guttation
Xylem
Exudates and Transudates
Hordeum
xylem
barley
Ions
ions
transporters
leaves
Plant Cells
Hordeum vulgare
tissues
fluids
transpiration
Growth

Keywords

  • Epithem cell
  • Hydathode
  • Ion nutrition
  • Phosphate
  • Vascular bundle
  • Xylem fluid.

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

Nagai, M., Ohnishi, M., Uehara, T., Yamagami, M., Miura, E., Kamakura, M., ... Mimura, T. (2013). Ion gradients in xylem exudate and guttation fluid related to tissue ion levels along primary leaves of barley. Plant, Cell and Environment, 36(10), 1826-1837. https://doi.org/10.1111/pce.12090

Ion gradients in xylem exudate and guttation fluid related to tissue ion levels along primary leaves of barley. / Nagai, Makiko; Ohnishi, Miwa; Uehara, Takeo; Yamagami, Mutsumi; Miura, Eiko; Kamakura, Mai; Kitamura, Akira; Sakaguchi, Shu Ichi; Sakamoto, Wataru; Shimmen, Teruo; Fukaki, Hidehiro; Reid, Robert J.; Furukawa, Akio; Mimura, Tetsuro.

In: Plant, Cell and Environment, Vol. 36, No. 10, 10.2013, p. 1826-1837.

Research output: Contribution to journalArticle

Nagai, M, Ohnishi, M, Uehara, T, Yamagami, M, Miura, E, Kamakura, M, Kitamura, A, Sakaguchi, SI, Sakamoto, W, Shimmen, T, Fukaki, H, Reid, RJ, Furukawa, A & Mimura, T 2013, 'Ion gradients in xylem exudate and guttation fluid related to tissue ion levels along primary leaves of barley', Plant, Cell and Environment, vol. 36, no. 10, pp. 1826-1837. https://doi.org/10.1111/pce.12090
Nagai, Makiko ; Ohnishi, Miwa ; Uehara, Takeo ; Yamagami, Mutsumi ; Miura, Eiko ; Kamakura, Mai ; Kitamura, Akira ; Sakaguchi, Shu Ichi ; Sakamoto, Wataru ; Shimmen, Teruo ; Fukaki, Hidehiro ; Reid, Robert J. ; Furukawa, Akio ; Mimura, Tetsuro. / Ion gradients in xylem exudate and guttation fluid related to tissue ion levels along primary leaves of barley. In: Plant, Cell and Environment. 2013 ; Vol. 36, No. 10. pp. 1826-1837.
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