Ion Channels Regulate Nyctinastic Leaf Opening in Samanea saman

Takaya Oikawa, Yasuhiro Ishimaru, Shintaro Munemasa, Yusuke Takeuchi, Kento Washiyama, Shin Hamamoto, Nobuyuki Yoshikawa, Yoshiyuki Mutara, Nobuyuki Uozumi, Minoru Ueda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The circadian leaf opening and closing (nyctinasty) of Fabaceae has attracted scientists’ attention since the era of Charles Darwin. Nyctinastic movement is triggered by the alternate swelling and shrinking of motor cells at the base of the leaf. This, in turn, is facilitated by changing osmotic pressures brought about by ion flow through anion and potassium ion channels. However, key regulatory ion channels and molecular mechanisms remain largely unknown. Here, we identify three key ion channels in mimosoid tree Samanea saman: the slow-type anion channels, SsSLAH1 and SsSLAH3, and the Shaker-type potassium channel, SPORK2. We show that cell-specific circadian expression of SsSLAH1 plays a key role in nyctinastic leaf opening. In addition, SsSLAH1 co-expressed with SsSLAH3 in flexor (abaxial) motor cells promoted leaf opening. We confirm the importance of SLAH1 in leaf movement using SLAH1-impaired Glycine max. Identification of this “master player” advances our molecular understanding of nyctinasty.

Original languageEnglish
JournalCurrent Biology
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Samanea saman
ion channels
Ion Channels
nastic movement
Anions
Shaker Superfamily of Potassium Channels
Potassium Channels
Glycine
Swelling
leaves
anions
Osmotic Pressure
Ions
Soybeans
Fabaceae
potassium channels
cells
osmotic pressure
Glycine max
potassium

Keywords

  • circadian rhythm
  • ion channel
  • nyctinasty
  • Samanea saman

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Oikawa, T., Ishimaru, Y., Munemasa, S., Takeuchi, Y., Washiyama, K., Hamamoto, S., ... Ueda, M. (Accepted/In press). Ion Channels Regulate Nyctinastic Leaf Opening in Samanea saman. Current Biology. https://doi.org/10.1016/j.cub.2018.05.042

Ion Channels Regulate Nyctinastic Leaf Opening in Samanea saman. / Oikawa, Takaya; Ishimaru, Yasuhiro; Munemasa, Shintaro; Takeuchi, Yusuke; Washiyama, Kento; Hamamoto, Shin; Yoshikawa, Nobuyuki; Mutara, Yoshiyuki; Uozumi, Nobuyuki; Ueda, Minoru.

In: Current Biology, 01.01.2018.

Research output: Contribution to journalArticle

Oikawa, T, Ishimaru, Y, Munemasa, S, Takeuchi, Y, Washiyama, K, Hamamoto, S, Yoshikawa, N, Mutara, Y, Uozumi, N & Ueda, M 2018, 'Ion Channels Regulate Nyctinastic Leaf Opening in Samanea saman', Current Biology. https://doi.org/10.1016/j.cub.2018.05.042
Oikawa, Takaya ; Ishimaru, Yasuhiro ; Munemasa, Shintaro ; Takeuchi, Yusuke ; Washiyama, Kento ; Hamamoto, Shin ; Yoshikawa, Nobuyuki ; Mutara, Yoshiyuki ; Uozumi, Nobuyuki ; Ueda, Minoru. / Ion Channels Regulate Nyctinastic Leaf Opening in Samanea saman. In: Current Biology. 2018.
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