Ion channels in basolateral membrane of marginal cells dissociated from gerbil stria vascularis

Shunji Takeuchi, Motonori Ando, Kenichi Kozakura, Haruo Saito, Akihiko Irimajiri

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Abstract

The basolateral membrane of isolated strial marginal cells has been probed for conductive pathways by the patch-clamp technique. Two types of voltage-insensitive channels were identified in both cell-attached and excised patches. Of these, frequently (69% of excised patches) observed was a Ca2+-activated nonselective cation channel having a unit conductance of 24.9 ± 0.5 pS (N = 16). Other characteristics of this type in excised patches include: 1) linear I-V relations with 150 mM K+ (pipette)/150 mM Na+ (bath), 2) a permeability sequence of NH4+> Na+ = K+ = Rb+>Li+, 3) a flickering block by quinine or quinidine (both 1 mM), and 4) a dose dependent block of its activity by ADP or ATP (IC50,ATP/IC50,ADP = 20-35), both from the cytosolic side. Channels with similar characteristics were found in the apical membrane of the same cell; however, the basolateral channels were 2-4 times more densely distributed than the apical counterparts. Also frequently (57%) detected was a Cl- channel of 80.0 ± 0.5 pS (N = 6), whose activity was Ca2+ independent. Additionally, this Cl- channel had: 1) linear I-V relations with symmetric Cl-, 2) a permeability sequence of Cl->Br->I-≥NO3-≥gluconate-, and 3) a complete and reversible block by 1 mM diphenylamine-2-carboxylate. In contrast to the apical Cl- channels, the basolateral ones had a much higher density (57% vs. <1%) as well as a higher unit conductance (80 pS vs. 50 pS) than the apical counterpart. The relative abundance of these two types as the major conductive pathways for Na+, K+, and Cl- in the basolateral region must be taken into account when addressing the role of strial marginal cells in generating the positive endocochlear potential. The Cl- channel may facilitate Cl- distribution across the basolateral membrane.

Original languageEnglish
Pages (from-to)89-100
Number of pages12
JournalHearing Research
Volume83
Issue number1-2
DOIs
Publication statusPublished - 1995
Externally publishedYes

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Keywords

  • Cl channel
  • Ion transport
  • Marginal cell
  • Nonselective cation channel

ASJC Scopus subject areas

  • Sensory Systems

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