Inwardly rectifying K+ currents in intermediate cells in the cochlea of gerbils: A possible contribution to the endocochlear potential

Shunji Takeuchi, Motonori Ando

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The stria vascularis in the cochlea generates the endocochlear potential (EP) and secretes K+-rich endolymph; both are indispensable for normal sound transduction by hair cells. K+ conductance in the intermediate cell, one of the several types of cells constituting the stria vascularis, was investigated by the whole-cell patch-clamp technique. Inwardly-rectifying K+ (K(ir)) currents were the major currents observed. The currents were inhibited dose-dependently by Ba2+, quinine, verapamil and Cs+, but not by tetraethylammonium (20 mM), 4-aminopyridine (5 mM) or Cd2+ (1 mM). The similarity between the effect of inhibitors on Kit currents and on the EP (Takeuchi et al., Hearing Res., 101 (1996) 181-185) suggests a direct contribution of the K(ir) conductance to the generation of the EP.

Original languageEnglish
Pages (from-to)175-178
Number of pages4
JournalNeuroscience Letters
Volume247
Issue number2-3
DOIs
Publication statusPublished - May 15 1998
Externally publishedYes

Fingerprint

Gerbillinae
Cochlea
Stria Vascularis
Endolymph
4-Aminopyridine
Tetraethylammonium
Quinine
Patch-Clamp Techniques
Verapamil
Hearing

Keywords

  • 4-aminopyridine
  • K(ir) channel
  • Melanocyte
  • Quinine
  • Stria vascularis
  • Tetraethylammonium
  • Verapamil

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Inwardly rectifying K+ currents in intermediate cells in the cochlea of gerbils : A possible contribution to the endocochlear potential. / Takeuchi, Shunji; Ando, Motonori.

In: Neuroscience Letters, Vol. 247, No. 2-3, 15.05.1998, p. 175-178.

Research output: Contribution to journalArticle

@article{b9139ed0afdf4c21980c47fb40a7af81,
title = "Inwardly rectifying K+ currents in intermediate cells in the cochlea of gerbils: A possible contribution to the endocochlear potential",
abstract = "The stria vascularis in the cochlea generates the endocochlear potential (EP) and secretes K+-rich endolymph; both are indispensable for normal sound transduction by hair cells. K+ conductance in the intermediate cell, one of the several types of cells constituting the stria vascularis, was investigated by the whole-cell patch-clamp technique. Inwardly-rectifying K+ (K(ir)) currents were the major currents observed. The currents were inhibited dose-dependently by Ba2+, quinine, verapamil and Cs+, but not by tetraethylammonium (20 mM), 4-aminopyridine (5 mM) or Cd2+ (1 mM). The similarity between the effect of inhibitors on Kit currents and on the EP (Takeuchi et al., Hearing Res., 101 (1996) 181-185) suggests a direct contribution of the K(ir) conductance to the generation of the EP.",
keywords = "4-aminopyridine, K(ir) channel, Melanocyte, Quinine, Stria vascularis, Tetraethylammonium, Verapamil",
author = "Shunji Takeuchi and Motonori Ando",
year = "1998",
month = "5",
day = "15",
doi = "10.1016/S0304-3940(98)00318-8",
language = "English",
volume = "247",
pages = "175--178",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "2-3",

}

TY - JOUR

T1 - Inwardly rectifying K+ currents in intermediate cells in the cochlea of gerbils

T2 - A possible contribution to the endocochlear potential

AU - Takeuchi, Shunji

AU - Ando, Motonori

PY - 1998/5/15

Y1 - 1998/5/15

N2 - The stria vascularis in the cochlea generates the endocochlear potential (EP) and secretes K+-rich endolymph; both are indispensable for normal sound transduction by hair cells. K+ conductance in the intermediate cell, one of the several types of cells constituting the stria vascularis, was investigated by the whole-cell patch-clamp technique. Inwardly-rectifying K+ (K(ir)) currents were the major currents observed. The currents were inhibited dose-dependently by Ba2+, quinine, verapamil and Cs+, but not by tetraethylammonium (20 mM), 4-aminopyridine (5 mM) or Cd2+ (1 mM). The similarity between the effect of inhibitors on Kit currents and on the EP (Takeuchi et al., Hearing Res., 101 (1996) 181-185) suggests a direct contribution of the K(ir) conductance to the generation of the EP.

AB - The stria vascularis in the cochlea generates the endocochlear potential (EP) and secretes K+-rich endolymph; both are indispensable for normal sound transduction by hair cells. K+ conductance in the intermediate cell, one of the several types of cells constituting the stria vascularis, was investigated by the whole-cell patch-clamp technique. Inwardly-rectifying K+ (K(ir)) currents were the major currents observed. The currents were inhibited dose-dependently by Ba2+, quinine, verapamil and Cs+, but not by tetraethylammonium (20 mM), 4-aminopyridine (5 mM) or Cd2+ (1 mM). The similarity between the effect of inhibitors on Kit currents and on the EP (Takeuchi et al., Hearing Res., 101 (1996) 181-185) suggests a direct contribution of the K(ir) conductance to the generation of the EP.

KW - 4-aminopyridine

KW - K(ir) channel

KW - Melanocyte

KW - Quinine

KW - Stria vascularis

KW - Tetraethylammonium

KW - Verapamil

UR - http://www.scopus.com/inward/record.url?scp=0032524626&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032524626&partnerID=8YFLogxK

U2 - 10.1016/S0304-3940(98)00318-8

DO - 10.1016/S0304-3940(98)00318-8

M3 - Article

C2 - 9655621

AN - SCOPUS:0032524626

VL - 247

SP - 175

EP - 178

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 2-3

ER -