Involvement of xanthine oxidoreductase-related oxidative stress in a Dermatophagoides farinae-induced asthma model of NC/Nga mice

Heri Setiawan, Kenjiro Nagaoka, Masayuki Kubo, Yoshihisa Fujikura, Keiki Ogino

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Oxidative stress is widely known to play a role in asthma. However, the contribution of xanthine oxidoreductase (XOR) as a source of the superoxide anion radical (O2 -) in oxidative stress associated with asthma has not yet been examined in detail. Here we investigated pathophysiological changes in XOR in an experimental model of asthma induced by the house dust mite Dermatophagoides farinae (Df). In the lungs of Df-treated mice, the production of O2 - from XOR increased and the nitrite concentrations decreased, whereas the protein expression of XOR remained unchanged. Moreover, the protein expression levels of XOR and the hydrogen peroxide (H2O2) concentrations in bronchoalveolar lavage fluid (BALF) were higher in the Df-treated mice than in saline-treated mice. Immunohistochemically, although XOR was highly localized in the bronchial epithelial cells of the saline-treated mice, immunostaining for XOR was absent in the bronchial epithelium of Df-treated mice. These results suggest that oxidative stress is up-regulated by increases in the conversion of the dehydrogenase form (xanthine dehydrogenase; XDH) of XOR to the oxidase form (xanthine oxidase; XOD).

Original languageEnglish
Pages (from-to)175-182
Number of pages8
JournalActa Medica Okayama
Volume70
Issue number3
Publication statusPublished - 2016

Fingerprint

Dermatophagoides farinae
Xanthine Dehydrogenase
Oxidative stress
Oxidative Stress
Asthma
Superoxides
Oxidoreductases
Pyroglyphidae
Xanthine Oxidase
Bronchoalveolar Lavage Fluid
Nitrites
Hydrogen Peroxide
Dust
Proteins
Theoretical Models
Epithelium
Epithelial Cells

Keywords

  • Asthma
  • Oxidative stress
  • Xanthine oxidase

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Involvement of xanthine oxidoreductase-related oxidative stress in a Dermatophagoides farinae-induced asthma model of NC/Nga mice. / Setiawan, Heri; Nagaoka, Kenjiro; Kubo, Masayuki; Fujikura, Yoshihisa; Ogino, Keiki.

In: Acta Medica Okayama, Vol. 70, No. 3, 2016, p. 175-182.

Research output: Contribution to journalArticle

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