Involvement of the NMDA-nitric oxide pathway in the development of hypersensitivity to tactile stimulation in dental injured rats

Norifumi Yonehara, Katsuhiko Amano, Yoshinori Kamisaki

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

To investigate mechanisms in pathological pain conditions as the hyperalgesia and allodynia observed after dental surgery, we employed a rat dental-injury model involving the simultaneous pulpectomy to a lower incisor and extraction of an ipsilateral upper incisor. We found that hypersensitivity to tactile stimulation developed on both ipsilateral and contralateral sides in the dental-injured rats 5 days after the surgery and that this lasted for at least 30 days. Recovery from hypersensitivity to tactile stimulation was achieved by the intraperitoneal (i.p.) administration of MK-801 (0.05 mg/kg) or NG-monomethyl-L-arginine monoacetate (L-NMMA: 10-100 mg/kg), but not attained by NG-monomethyl-D-arginine monoacetate (D-NMMA: 100 mg/kg). This recovery effect of L-NMMA (50 mg/kg) was inhibited by pretreatment with L-arginine (600 mg/kg). In the trigeminal nucleus caudalis (SpVc), the changes in nitric oxide (NO) levels invoked by the intravenous (i.v.) administration of N-methyl-D-aspartate (NMDA; 10 mg/kg) were found to be significantly larger in the dental-injured rats than in sham-operated rats. The number of neuronal NO synthase (nNOS)-positive neurons increased in layers I-II and III-IV in the SpVc on both sides of the dental-injured rats. These results suggest that hypersensitivity to tactile stimulation developed following dental injury, and that NMDA receptor/NOS/NO production pathways in the SpVc may be involved in pathological conditions.

Original languageEnglish
Pages (from-to)145-155
Number of pages11
JournalJapanese Journal of Pharmacology
Volume90
Issue number2
DOIs
Publication statusPublished - Oct 1 2002
Externally publishedYes

Keywords

  • Dental injury
  • Hypersensitivity to tactile stimulation
  • Nitric oxide
  • NMDA receptor
  • Trigeminal nucleus caudalis

ASJC Scopus subject areas

  • Pharmacology

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