Involvement of the chromosomal translocation t(11;18) in some mucosa-associated lymphoid tissue lymphomas and diffuse large B-cell lymphomas of the ocular adnexa evidence from multiplex reverse transcriptase-polymerase chain reaction and fluorescence in situ hybridization on using formalin-fixed, paraffin-embedded specimens

Shinichi Takada, Tadashi Yoshino, Masafumi Taniwaki, Naoya Nakamura, Hirokazu Nakamine, Koichi Oshima, Yoshito Sadahira, Hiroshi Inagaki, Akagi Tadaatsu

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The chromosomal translocation t(11;18) is a unique chromosomal aberration associated with mucosa-associated lymphoid tissue lymphoma. API2 and MALT1 genes have been identified around this translocation. We attempted to find chromosomal abnormalities focusing mainly on the t(11;18) translocation in formalin-fixed, paraffin-embedded tissues of ocular adnexal lymphoproliferative disorders using multiplex reverse transcriptase-polymerase chain reaction and/or two-color interphase fluorescence in situ hybridization. By these methods, the t(11;18) translocation was detected in 1 of 8 patients with reactive lymphoid hyperplasia (13%), 3 of 23 with mucosa-associated lymphoid tissue lymphoma (13%), and 2 of 14 with diffuse large B-cell lymphoma with/without mucosa-associated lymphoid tissue lymphoma (14%). Moreover, we performed fluorescence in situ hybridization analysis to detect any numerical aberration of chromosomes 3, 7, 12, and 18 on some specimens nonselectively. No numerical chromosomal abnormalities were detected in 3 cases of reactive lymphoid hyperplasia, whereas three of four cases of mucosa-associated lymphoid tissue lymphoma and all four cases of diffuse large B-cell lymphoma with/without mucosa-associated lymphoid tissue lymphoma components exhibited one or more abnormalities. These findings indicate a possibility that at least in the ocular adnexa, some diffuse large B-cell lymphomas are derived from mucosa-associated lymphoid tissue lymphomas.

Original languageEnglish
Pages (from-to)445-452
Number of pages8
JournalModern Pathology
Volume16
Issue number5
DOIs
Publication statusPublished - May 1 2003

Fingerprint

Marginal Zone B-Cell Lymphoma
Genetic Translocation
Lymphoma, Large B-Cell, Diffuse
Multiplex Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Fluorescence In Situ Hybridization
Paraffin
Formaldehyde
Chromosome Aberrations
Pseudolymphoma
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 7
Lymphoproliferative Disorders
Interphase
B-Lymphocytes
Color
Genes

Keywords

  • Chromosomal translocation
  • Diffuse large B-cell lymphoma
  • Fluorescence in situ hybridization
  • Mucosa-associated lymphoid tissue lymphoma
  • Ocular adnexa
  • RT-PCR

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Involvement of the chromosomal translocation t(11;18) in some mucosa-associated lymphoid tissue lymphomas and diffuse large B-cell lymphomas of the ocular adnexa evidence from multiplex reverse transcriptase-polymerase chain reaction and fluorescence in situ hybridization on using formalin-fixed, paraffin-embedded specimens. / Takada, Shinichi; Yoshino, Tadashi; Taniwaki, Masafumi; Nakamura, Naoya; Nakamine, Hirokazu; Oshima, Koichi; Sadahira, Yoshito; Inagaki, Hiroshi; Tadaatsu, Akagi.

In: Modern Pathology, Vol. 16, No. 5, 01.05.2003, p. 445-452.

Research output: Contribution to journalArticle

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abstract = "The chromosomal translocation t(11;18) is a unique chromosomal aberration associated with mucosa-associated lymphoid tissue lymphoma. API2 and MALT1 genes have been identified around this translocation. We attempted to find chromosomal abnormalities focusing mainly on the t(11;18) translocation in formalin-fixed, paraffin-embedded tissues of ocular adnexal lymphoproliferative disorders using multiplex reverse transcriptase-polymerase chain reaction and/or two-color interphase fluorescence in situ hybridization. By these methods, the t(11;18) translocation was detected in 1 of 8 patients with reactive lymphoid hyperplasia (13{\%}), 3 of 23 with mucosa-associated lymphoid tissue lymphoma (13{\%}), and 2 of 14 with diffuse large B-cell lymphoma with/without mucosa-associated lymphoid tissue lymphoma (14{\%}). Moreover, we performed fluorescence in situ hybridization analysis to detect any numerical aberration of chromosomes 3, 7, 12, and 18 on some specimens nonselectively. No numerical chromosomal abnormalities were detected in 3 cases of reactive lymphoid hyperplasia, whereas three of four cases of mucosa-associated lymphoid tissue lymphoma and all four cases of diffuse large B-cell lymphoma with/without mucosa-associated lymphoid tissue lymphoma components exhibited one or more abnormalities. These findings indicate a possibility that at least in the ocular adnexa, some diffuse large B-cell lymphomas are derived from mucosa-associated lymphoid tissue lymphomas.",
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AU - Takada, Shinichi

AU - Yoshino, Tadashi

AU - Taniwaki, Masafumi

AU - Nakamura, Naoya

AU - Nakamine, Hirokazu

AU - Oshima, Koichi

AU - Sadahira, Yoshito

AU - Inagaki, Hiroshi

AU - Tadaatsu, Akagi

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