Involvement of superoxide generation in salicylic acid-induced stomatal closure in Vicia faba

Izumi Mori, Reinhard Pinontoan, Tomonori Kawano, Shoshi Muto

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Salicylic acid (SA), the known mediator of systemic acquired resistance, induced stomatal closure of Vicia faba L. Application of SA to the epidermal peels evoked an elevation of chemiluminescence of Cripridina lucigenin-derived chemiluminescent reagent (CLA) which is sensitive to superoxide anion (O2·-). The SA-induced generation of chemiluminescence was suppressed by O2·-specific scavengers superoxide dismutase (SOD) and 4,5-dihydroxy-1,3-benzenedisulfonic acid (Tiron). These results suggest that O2·- was generated in epidermal peels by SA-treatment. A peroxidase inhibitor salicylhydroxamic acid (SHAM) inhibited guaiacol peroxidase activity and suppressed the SA-induced CLA chemiluminescence in the epidermal peels, suggesting that O2·- generation occurred by the peroxidasecatalyzed reaction as proposed for SA-treated tobacco cell suspension culture [Kawano et al. (1998) Plant Cell Physiol. 39: 721]. SOD, Tiron or SHAM suppressed the SA-induced stomatal closure. Moreover, application of superoxidegenerating system also induced stomatal closure. These results support the concept of involvement of reactive oxygen species in signal transduction in SA-induced stomatal closure.

Original languageEnglish
Pages (from-to)1383-1388
Number of pages6
JournalPlant and Cell Physiology
Volume42
Issue number12
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Vicia faba
Salicylic Acid
salicylic acid
Superoxides
superoxide anion
chemiluminescence
Luminescence
1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt
Superoxide Dismutase
acids
superoxide dismutase
peroxidase
systemic acquired resistance
Plant Cells
cell suspension culture
Peroxidase
Tobacco
signal transduction
reactive oxygen species
Reactive Oxygen Species

Keywords

  • Peroxidase
  • Salicylic acid
  • Stomatal closure
  • Superoxide anion
  • Vicia faba

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Involvement of superoxide generation in salicylic acid-induced stomatal closure in Vicia faba. / Mori, Izumi; Pinontoan, Reinhard; Kawano, Tomonori; Muto, Shoshi.

In: Plant and Cell Physiology, Vol. 42, No. 12, 2001, p. 1383-1388.

Research output: Contribution to journalArticle

Mori, Izumi ; Pinontoan, Reinhard ; Kawano, Tomonori ; Muto, Shoshi. / Involvement of superoxide generation in salicylic acid-induced stomatal closure in Vicia faba. In: Plant and Cell Physiology. 2001 ; Vol. 42, No. 12. pp. 1383-1388.
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