Involvement of STAT3 in bladder smooth muscle hypertrophy following bladder outlet obstruction

Osamu Fujita, Masato Asanuma, Teruhiko Yokoyama, Ikuko Miyazaki, Norio Ogawa, Hiromi Kumon

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We examined the involvement of the signal transducer and activator of transcription 3 (STAT3) in bladder outlet obstruction (BOO)-induced bladder smooth muscle hypertrophy using a rat in vivo and in vitro study. BOO induced increases in bladder weight and bladder smooth muscle thickness 1 week after the operation. By using antibody microarrays, 64 of 389 proteins blotted on the array met our selection criteria of an INR value between ≥ 2.0 and ≤ 0.5. This result revealed up-regulation of transcription factors, cell cycle regulatory proteins, apoptosis-associated proteins and so on. On the other hand, down-regulation (INR value ≤ 0.5) of proteins was not found. In a profiling study, we found an increase in the expression of STAT3. A significant increase in nuclear phosphorylated STAT3 expression was confirmed in bladder smooth muscle tissue by immunohistochemistry and Western blot analysis. Cyclical stretch-relaxation (1 Hz) at 120% elongation significantly increased the expression of STAT3 and of α-smooth muscle actin in primary cultured bladder smooth muscle cells. Furthermore, the blockade of STAT3 expression by the transfection of STAT3 small interfering RNA (siRNA) significantly prevented the stretch-induced increase in α-smooth muscle actin expression. These results suggest that STAT3 has an important role in the induction of bladder smooth muscle hypertrophy. Copyright

Original languageEnglish
Pages (from-to)299-309
Number of pages11
JournalActa Medica Okayama
Volume60
Issue number6
Publication statusPublished - Dec 2006

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Urinary Bladder Neck Obstruction
STAT3 Transcription Factor
Hypertrophy
Smooth Muscle
Muscle
Urinary Bladder
International Normalized Ratio
Actins
Cell Cycle Proteins
Proteins
Microarrays
Patient Selection
Small Interfering RNA
Smooth Muscle Myocytes
Transfection
Rats
Elongation
Transcription Factors
Up-Regulation
Down-Regulation

Keywords

  • Benign prostatic hyperplasia
  • Bladder outlet obstruction
  • Bladder smooth muscle
  • Signal transducer and activator of transcription 3 (STAT3)
  • Small interfering RNA (siRNA)

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Involvement of STAT3 in bladder smooth muscle hypertrophy following bladder outlet obstruction. / Fujita, Osamu; Asanuma, Masato; Yokoyama, Teruhiko; Miyazaki, Ikuko; Ogawa, Norio; Kumon, Hiromi.

In: Acta Medica Okayama, Vol. 60, No. 6, 12.2006, p. 299-309.

Research output: Contribution to journalArticle

Fujita, Osamu ; Asanuma, Masato ; Yokoyama, Teruhiko ; Miyazaki, Ikuko ; Ogawa, Norio ; Kumon, Hiromi. / Involvement of STAT3 in bladder smooth muscle hypertrophy following bladder outlet obstruction. In: Acta Medica Okayama. 2006 ; Vol. 60, No. 6. pp. 299-309.
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