Involvement of STAT3 in bladder smooth muscle hypertrophy following bladder outlet obstruction

Osamu Fujita, Masato Asanuma, Teruhiko Yokoyama, Ikuko Miyazaki, Norio Ogawa, Hiromi Kumon

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

We examined the involvement of the signal transducer and activator of transcription 3 (STAT3) in bladder outlet obstruction (BOO)-induced bladder smooth muscle hypertrophy using a rat in vivo and in vitro study. BOO induced increases in bladder weight and bladder smooth muscle thickness 1 week after the operation. By using antibody microarrays, 64 of 389 proteins blotted on the array met our selection criteria of an INR value between ≥ 2.0 and ≤ 0.5. This result revealed up-regulation of transcription factors, cell cycle regulatory proteins, apoptosis-associated proteins and so on. On the other hand, down-regulation (INR value ≤ 0.5) of proteins was not found. In a profiling study, we found an increase in the expression of STAT3. A significant increase in nuclear phosphorylated STAT3 expression was confirmed in bladder smooth muscle tissue by immunohistochemistry and Western blot analysis. Cyclical stretch-relaxation (1 Hz) at 120% elongation significantly increased the expression of STAT3 and of α-smooth muscle actin in primary cultured bladder smooth muscle cells. Furthermore, the blockade of STAT3 expression by the transfection of STAT3 small interfering RNA (siRNA) significantly prevented the stretch-induced increase in α-smooth muscle actin expression. These results suggest that STAT3 has an important role in the induction of bladder smooth muscle hypertrophy. Copyright

Original languageEnglish
Pages (from-to)299-309
Number of pages11
JournalActa medica Okayama
Volume60
Issue number6
Publication statusPublished - Dec 2006

Keywords

  • Benign prostatic hyperplasia
  • Bladder outlet obstruction
  • Bladder smooth muscle
  • Signal transducer and activator of transcription 3 (STAT3)
  • Small interfering RNA (siRNA)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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