Involvement of silicon influx transporter OsNIP2;1 in selenite uptake in rice

Xue Qiang Zhao, Namiki Mitani, Naoki Yamaji, Ren Fang Shen, Jian Feng Ma

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Rice (Oryza sativa) as a staple food, provides a major source of dietary selenium (Se) for humans, which essentially requires Se, however, the molecular mechanism for Se uptake is still poorly understood. Herein, we show evidence that the uptake of selenite, a main bioavailable form of Se in paddy soils, is mediated by a silicon (Si) influx transporter Lsi1 (OsNIP2;1) in rice. Defect of OsNIP2;1 resulted in a significant decrease in the Se concentration of the shoots and xylem sap when selenite was given. However, there was no difference in the Se concentration between the wild-type rice and mutant of OsNIP2;1 when selenate was supplied. A short-term uptake experiment showed that selenite uptake greatly increased with decreasing pH in the external solution. Si as silicic acid did not inhibit the Se uptake from selenite in both rice and yeast (Saccharomyces cerevisiae) at low pHs. Expression of OsNIP2;1 in yeast enhanced the selenite uptake at pH 3.5 and 5.5 but not at pH 7.5. On the other hand, defect of Si efflux transporter Lsi2 did not affect the uptake of Se either from selenite or selenate. Taken together, our results indicate that Si influx transporter OsNIP2;1 is permeable to selenite.

Original languageEnglish
Pages (from-to)1871-1877
Number of pages7
JournalPlant Physiology
Volume153
Issue number4
DOIs
Publication statusPublished - Aug 2010

Fingerprint

Selenious Acid
selenites
Silicon
silicon
Selenium
selenium
transporters
rice
Selenic Acid
selenates
Yeasts
Silicic Acid
yeasts
Xylem
silicic acid
Oryza
paddy soils
staple foods
sap
xylem

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology
  • Medicine(all)

Cite this

Involvement of silicon influx transporter OsNIP2;1 in selenite uptake in rice. / Zhao, Xue Qiang; Mitani, Namiki; Yamaji, Naoki; Shen, Ren Fang; Ma, Jian Feng.

In: Plant Physiology, Vol. 153, No. 4, 08.2010, p. 1871-1877.

Research output: Contribution to journalArticle

@article{860711def1654192b6480f9826c95d83,
title = "Involvement of silicon influx transporter OsNIP2;1 in selenite uptake in rice",
abstract = "Rice (Oryza sativa) as a staple food, provides a major source of dietary selenium (Se) for humans, which essentially requires Se, however, the molecular mechanism for Se uptake is still poorly understood. Herein, we show evidence that the uptake of selenite, a main bioavailable form of Se in paddy soils, is mediated by a silicon (Si) influx transporter Lsi1 (OsNIP2;1) in rice. Defect of OsNIP2;1 resulted in a significant decrease in the Se concentration of the shoots and xylem sap when selenite was given. However, there was no difference in the Se concentration between the wild-type rice and mutant of OsNIP2;1 when selenate was supplied. A short-term uptake experiment showed that selenite uptake greatly increased with decreasing pH in the external solution. Si as silicic acid did not inhibit the Se uptake from selenite in both rice and yeast (Saccharomyces cerevisiae) at low pHs. Expression of OsNIP2;1 in yeast enhanced the selenite uptake at pH 3.5 and 5.5 but not at pH 7.5. On the other hand, defect of Si efflux transporter Lsi2 did not affect the uptake of Se either from selenite or selenate. Taken together, our results indicate that Si influx transporter OsNIP2;1 is permeable to selenite.",
author = "Zhao, {Xue Qiang} and Namiki Mitani and Naoki Yamaji and Shen, {Ren Fang} and Ma, {Jian Feng}",
year = "2010",
month = "8",
doi = "10.1104/pp.110.157867",
language = "English",
volume = "153",
pages = "1871--1877",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "4",

}

TY - JOUR

T1 - Involvement of silicon influx transporter OsNIP2;1 in selenite uptake in rice

AU - Zhao, Xue Qiang

AU - Mitani, Namiki

AU - Yamaji, Naoki

AU - Shen, Ren Fang

AU - Ma, Jian Feng

PY - 2010/8

Y1 - 2010/8

N2 - Rice (Oryza sativa) as a staple food, provides a major source of dietary selenium (Se) for humans, which essentially requires Se, however, the molecular mechanism for Se uptake is still poorly understood. Herein, we show evidence that the uptake of selenite, a main bioavailable form of Se in paddy soils, is mediated by a silicon (Si) influx transporter Lsi1 (OsNIP2;1) in rice. Defect of OsNIP2;1 resulted in a significant decrease in the Se concentration of the shoots and xylem sap when selenite was given. However, there was no difference in the Se concentration between the wild-type rice and mutant of OsNIP2;1 when selenate was supplied. A short-term uptake experiment showed that selenite uptake greatly increased with decreasing pH in the external solution. Si as silicic acid did not inhibit the Se uptake from selenite in both rice and yeast (Saccharomyces cerevisiae) at low pHs. Expression of OsNIP2;1 in yeast enhanced the selenite uptake at pH 3.5 and 5.5 but not at pH 7.5. On the other hand, defect of Si efflux transporter Lsi2 did not affect the uptake of Se either from selenite or selenate. Taken together, our results indicate that Si influx transporter OsNIP2;1 is permeable to selenite.

AB - Rice (Oryza sativa) as a staple food, provides a major source of dietary selenium (Se) for humans, which essentially requires Se, however, the molecular mechanism for Se uptake is still poorly understood. Herein, we show evidence that the uptake of selenite, a main bioavailable form of Se in paddy soils, is mediated by a silicon (Si) influx transporter Lsi1 (OsNIP2;1) in rice. Defect of OsNIP2;1 resulted in a significant decrease in the Se concentration of the shoots and xylem sap when selenite was given. However, there was no difference in the Se concentration between the wild-type rice and mutant of OsNIP2;1 when selenate was supplied. A short-term uptake experiment showed that selenite uptake greatly increased with decreasing pH in the external solution. Si as silicic acid did not inhibit the Se uptake from selenite in both rice and yeast (Saccharomyces cerevisiae) at low pHs. Expression of OsNIP2;1 in yeast enhanced the selenite uptake at pH 3.5 and 5.5 but not at pH 7.5. On the other hand, defect of Si efflux transporter Lsi2 did not affect the uptake of Se either from selenite or selenate. Taken together, our results indicate that Si influx transporter OsNIP2;1 is permeable to selenite.

UR - http://www.scopus.com/inward/record.url?scp=77955672772&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955672772&partnerID=8YFLogxK

U2 - 10.1104/pp.110.157867

DO - 10.1104/pp.110.157867

M3 - Article

C2 - 20498338

AN - SCOPUS:77955672772

VL - 153

SP - 1871

EP - 1877

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 4

ER -