Involvement of second messengers in the signaling pathway of vitellogenesis-inhibiting hormone and their effects on vitellogenin mRNA expression in the whiteleg shrimp, Litopenaeus vannamei

Sun Hye Bae, Tomoyuki Okutsu, Naoaki Tsutsui, Bong Jung Kang, Hsiang Yin Chen, Marcy N. Wilder

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We incubated fragments of Litopenaeus vannamei ovary to investigate second messengers involved in the regulation of vitellogenin (vg) mRNA levels. The use of 100 nM recombinant vitellogenesis-inhibiting hormone (VIH) (corresponding to recombinant L. vannamei sinus gland peptide-G: rLiv-SGP-G) significantly reduced vg mRNA expression in sub-adults after 8 h incubation to less than 20% of the control. The concentration of intracellular cyclic guanosine monophosphate (cGMP) increased 3.2-fold relative to the control after 2 h incubation with rLiv-SGP-G. However, it reached levels 18-fold relative to the control after 0.5 h incubation with rLiv-SGP-G where 3-isobutyl-1-methylxanthine (a phosphodiesterase inhibitor) was also added. Moreover, vg mRNA expression was significantly reduced to less than 50% of the control after 24 h incubation with 1 μM A23187 (a calcium ionophore). Thus, rLiv-SGP-G and calcium ionophore reduced vg mRNA expression in in vitro-cultured ovary, and cGMP may be involved in the signaling pathway of VIH. Overall, the above results suggest that vg mRNA expression might be inhibited in vitro by increasing intracellular cGMP and Ca2+ in L. vannamei ovary.

Original languageEnglish
Pages (from-to)301-308
Number of pages8
JournalGeneral and Comparative Endocrinology
Volume246
DOIs
Publication statusPublished - May 15 2017

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Keywords

  • Calcium ionophore
  • Cyclic AMP
  • Cyclic GMP
  • Litopenaeus vannamei
  • Recombinant sinus gland peptide
  • Vitellogenin

ASJC Scopus subject areas

  • Endocrinology

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