Involvement of nitric oxide in light-mediated greening of barley seedlings

Lingang Zhang, Yading Wang, Liqun Zhao, Suyun Shi, Lixin Zhang

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

When seedlings are grown in the dark, proplastids of the developing leaf differentiate into etioplasts. Greening of etiolated plastids is stimulated by light, which is sensed by various types of photoreceptors. Nitric oxide (NO) has been shown to be a bioactive molecule that could take part in this light-mediated process in plants. In this paper, we show that emission of NO in barley seedlings increased concomitantly with increasing activities of nitric oxide synthase (NOS) during the greening. Treatment with sodium nitroprusside (SNP), a NO donor, increased the accumulation of chlorophyll contents, enhanced the accumulation of thylakoid membrane proteins, such as light harvesting complex of photosystem II (LHCII) and PSIA/B, and then improved the effective quantum yield of photosystem II (PSII) (ΦPSII) in the light. Instead, treatment with either NO scavenger 2-phenyl-4,4,5,5-tetramentylimidazoline-1-oxyl-3-xide (PTIO) or NOS inhibitor Nω-nitro-l-arginine (l-NNA) retarded the greening of etiolated-seedlings. Moreover, sodium ferrocyanide, an analog of SNP, nitrite and nitrate, two NO-decomposition products did not have any effect on the greening process. These results indicated that NO, as an endogenous signaling molecule, participates in light-mediated greening of barley seedlings, and exogenous NO accelerates this process.

Original languageEnglish
Pages (from-to)818-826
Number of pages9
JournalJournal of Plant Physiology
Volume163
Issue number8
DOIs
Publication statusPublished - Jul 3 2006
Externally publishedYes

Fingerprint

Hordeum
Seedlings
nitric oxide
Nitric Oxide
barley
Light
Photosystem II Protein Complex
seedlings
photosystem II
Nitroprusside
Nitric Oxide Synthase
nitric oxide synthase
Thylakoid Membrane Proteins
Sodium Nitrite
ferrocyanides
Plastids
Nitric Oxide Donors
etioplasts
Chloroplasts
Chlorophyll

Keywords

  • Etiolated seedling
  • Greening
  • Nitric oxide
  • Signaling molecule

ASJC Scopus subject areas

  • Plant Science

Cite this

Involvement of nitric oxide in light-mediated greening of barley seedlings. / Zhang, Lingang; Wang, Yading; Zhao, Liqun; Shi, Suyun; Zhang, Lixin.

In: Journal of Plant Physiology, Vol. 163, No. 8, 03.07.2006, p. 818-826.

Research output: Contribution to journalArticle

Zhang, Lingang ; Wang, Yading ; Zhao, Liqun ; Shi, Suyun ; Zhang, Lixin. / Involvement of nitric oxide in light-mediated greening of barley seedlings. In: Journal of Plant Physiology. 2006 ; Vol. 163, No. 8. pp. 818-826.
@article{f246a582d037482682ea80edcdd25d2a,
title = "Involvement of nitric oxide in light-mediated greening of barley seedlings",
abstract = "When seedlings are grown in the dark, proplastids of the developing leaf differentiate into etioplasts. Greening of etiolated plastids is stimulated by light, which is sensed by various types of photoreceptors. Nitric oxide (NO) has been shown to be a bioactive molecule that could take part in this light-mediated process in plants. In this paper, we show that emission of NO in barley seedlings increased concomitantly with increasing activities of nitric oxide synthase (NOS) during the greening. Treatment with sodium nitroprusside (SNP), a NO donor, increased the accumulation of chlorophyll contents, enhanced the accumulation of thylakoid membrane proteins, such as light harvesting complex of photosystem II (LHCII) and PSIA/B, and then improved the effective quantum yield of photosystem II (PSII) (ΦPSII) in the light. Instead, treatment with either NO scavenger 2-phenyl-4,4,5,5-tetramentylimidazoline-1-oxyl-3-xide (PTIO) or NOS inhibitor Nω-nitro-l-arginine (l-NNA) retarded the greening of etiolated-seedlings. Moreover, sodium ferrocyanide, an analog of SNP, nitrite and nitrate, two NO-decomposition products did not have any effect on the greening process. These results indicated that NO, as an endogenous signaling molecule, participates in light-mediated greening of barley seedlings, and exogenous NO accelerates this process.",
keywords = "Etiolated seedling, Greening, Nitric oxide, Signaling molecule",
author = "Lingang Zhang and Yading Wang and Liqun Zhao and Suyun Shi and Lixin Zhang",
year = "2006",
month = "7",
day = "3",
doi = "10.1016/j.jplph.2005.07.011",
language = "English",
volume = "163",
pages = "818--826",
journal = "Journal of Plant Physiology",
issn = "0176-1617",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "8",

}

TY - JOUR

T1 - Involvement of nitric oxide in light-mediated greening of barley seedlings

AU - Zhang, Lingang

AU - Wang, Yading

AU - Zhao, Liqun

AU - Shi, Suyun

AU - Zhang, Lixin

PY - 2006/7/3

Y1 - 2006/7/3

N2 - When seedlings are grown in the dark, proplastids of the developing leaf differentiate into etioplasts. Greening of etiolated plastids is stimulated by light, which is sensed by various types of photoreceptors. Nitric oxide (NO) has been shown to be a bioactive molecule that could take part in this light-mediated process in plants. In this paper, we show that emission of NO in barley seedlings increased concomitantly with increasing activities of nitric oxide synthase (NOS) during the greening. Treatment with sodium nitroprusside (SNP), a NO donor, increased the accumulation of chlorophyll contents, enhanced the accumulation of thylakoid membrane proteins, such as light harvesting complex of photosystem II (LHCII) and PSIA/B, and then improved the effective quantum yield of photosystem II (PSII) (ΦPSII) in the light. Instead, treatment with either NO scavenger 2-phenyl-4,4,5,5-tetramentylimidazoline-1-oxyl-3-xide (PTIO) or NOS inhibitor Nω-nitro-l-arginine (l-NNA) retarded the greening of etiolated-seedlings. Moreover, sodium ferrocyanide, an analog of SNP, nitrite and nitrate, two NO-decomposition products did not have any effect on the greening process. These results indicated that NO, as an endogenous signaling molecule, participates in light-mediated greening of barley seedlings, and exogenous NO accelerates this process.

AB - When seedlings are grown in the dark, proplastids of the developing leaf differentiate into etioplasts. Greening of etiolated plastids is stimulated by light, which is sensed by various types of photoreceptors. Nitric oxide (NO) has been shown to be a bioactive molecule that could take part in this light-mediated process in plants. In this paper, we show that emission of NO in barley seedlings increased concomitantly with increasing activities of nitric oxide synthase (NOS) during the greening. Treatment with sodium nitroprusside (SNP), a NO donor, increased the accumulation of chlorophyll contents, enhanced the accumulation of thylakoid membrane proteins, such as light harvesting complex of photosystem II (LHCII) and PSIA/B, and then improved the effective quantum yield of photosystem II (PSII) (ΦPSII) in the light. Instead, treatment with either NO scavenger 2-phenyl-4,4,5,5-tetramentylimidazoline-1-oxyl-3-xide (PTIO) or NOS inhibitor Nω-nitro-l-arginine (l-NNA) retarded the greening of etiolated-seedlings. Moreover, sodium ferrocyanide, an analog of SNP, nitrite and nitrate, two NO-decomposition products did not have any effect on the greening process. These results indicated that NO, as an endogenous signaling molecule, participates in light-mediated greening of barley seedlings, and exogenous NO accelerates this process.

KW - Etiolated seedling

KW - Greening

KW - Nitric oxide

KW - Signaling molecule

UR - http://www.scopus.com/inward/record.url?scp=33744913013&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744913013&partnerID=8YFLogxK

U2 - 10.1016/j.jplph.2005.07.011

DO - 10.1016/j.jplph.2005.07.011

M3 - Article

C2 - 16777529

AN - SCOPUS:33744913013

VL - 163

SP - 818

EP - 826

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

SN - 0176-1617

IS - 8

ER -