Abstract
Objectives: Platelet-rich plasma (PRP) has been widely used to enhance the regeneration of damaged joint tissues, such as osteoarthritic and rheumatoid arthritic cartilage. The aim of this study is to clarify the involvement of all of the CCN family proteins that are crucially associated with joint tissue regeneration. Methods: Cyr61-CTGF-NOV (CCN) family proteins in human platelets and megakaryocytic cells were comprehensively analyzed by Western blotting analysis. Production of CCN family proteins in megakaryocytes in vivo was confirmed by immunofluorescence analysis of mouse bone marrow cells. Effects of CCN family proteins found in platelets on chondrocytes were evaluated by using human chondrocytic HCS-2/8 cells. Results: Inclusion of CCN2, a mesenchymal tissue regenerator, was confirmed. Of note, CCN3, which counteracts CCN2, was newly found to be encapsulated in platelets. Interestingly, these two family members were not detectable in megakaryocytic cells, but their external origins were suggested. Furthermore, we found for the first time CCN5 and CCN1 that inhibits ADAMTS4 in both platelets and megakaryocytes. Finally, application of a CCN family cocktail mimicking platelets onto HCS-2/8 cells enhanced their chondrocytic phenotype. Conclusions: Multiple inclusion of CCN1, 2 and 3 in platelets was clarified, which supports the harmonized regenerative potential of PRP in joint therapeutics.
| Original language | English |
|---|---|
| Pages (from-to) | 1-10 |
| Number of pages | 10 |
| Journal | Modern Rheumatology |
| DOIs | |
| Publication status | Accepted/In press - Apr 6 2016 |
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Keywords
- Cartilage
- CCN family
- Megakaryocyte
- Platelet
- Regeneration
ASJC Scopus subject areas
- Rheumatology
Cite this
Involvement of multiple CCN family members in platelets that support regeneration of joint tissues. / Hara, Chikako; Kubota, Satoshi; Nishida, Takashi; Hiasa, Miki; Hattori, Takako; Aoyama, Eriko; Moriyama, Yoshinori; Kamioka, Hiroshi; Takigawa, Masaharu.
In: Modern Rheumatology, 06.04.2016, p. 1-10.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Involvement of multiple CCN family members in platelets that support regeneration of joint tissues
AU - Hara, Chikako
AU - Kubota, Satoshi
AU - Nishida, Takashi
AU - Hiasa, Miki
AU - Hattori, Takako
AU - Aoyama, Eriko
AU - Moriyama, Yoshinori
AU - Kamioka, Hiroshi
AU - Takigawa, Masaharu
PY - 2016/4/6
Y1 - 2016/4/6
N2 - Objectives: Platelet-rich plasma (PRP) has been widely used to enhance the regeneration of damaged joint tissues, such as osteoarthritic and rheumatoid arthritic cartilage. The aim of this study is to clarify the involvement of all of the CCN family proteins that are crucially associated with joint tissue regeneration. Methods: Cyr61-CTGF-NOV (CCN) family proteins in human platelets and megakaryocytic cells were comprehensively analyzed by Western blotting analysis. Production of CCN family proteins in megakaryocytes in vivo was confirmed by immunofluorescence analysis of mouse bone marrow cells. Effects of CCN family proteins found in platelets on chondrocytes were evaluated by using human chondrocytic HCS-2/8 cells. Results: Inclusion of CCN2, a mesenchymal tissue regenerator, was confirmed. Of note, CCN3, which counteracts CCN2, was newly found to be encapsulated in platelets. Interestingly, these two family members were not detectable in megakaryocytic cells, but their external origins were suggested. Furthermore, we found for the first time CCN5 and CCN1 that inhibits ADAMTS4 in both platelets and megakaryocytes. Finally, application of a CCN family cocktail mimicking platelets onto HCS-2/8 cells enhanced their chondrocytic phenotype. Conclusions: Multiple inclusion of CCN1, 2 and 3 in platelets was clarified, which supports the harmonized regenerative potential of PRP in joint therapeutics.
AB - Objectives: Platelet-rich plasma (PRP) has been widely used to enhance the regeneration of damaged joint tissues, such as osteoarthritic and rheumatoid arthritic cartilage. The aim of this study is to clarify the involvement of all of the CCN family proteins that are crucially associated with joint tissue regeneration. Methods: Cyr61-CTGF-NOV (CCN) family proteins in human platelets and megakaryocytic cells were comprehensively analyzed by Western blotting analysis. Production of CCN family proteins in megakaryocytes in vivo was confirmed by immunofluorescence analysis of mouse bone marrow cells. Effects of CCN family proteins found in platelets on chondrocytes were evaluated by using human chondrocytic HCS-2/8 cells. Results: Inclusion of CCN2, a mesenchymal tissue regenerator, was confirmed. Of note, CCN3, which counteracts CCN2, was newly found to be encapsulated in platelets. Interestingly, these two family members were not detectable in megakaryocytic cells, but their external origins were suggested. Furthermore, we found for the first time CCN5 and CCN1 that inhibits ADAMTS4 in both platelets and megakaryocytes. Finally, application of a CCN family cocktail mimicking platelets onto HCS-2/8 cells enhanced their chondrocytic phenotype. Conclusions: Multiple inclusion of CCN1, 2 and 3 in platelets was clarified, which supports the harmonized regenerative potential of PRP in joint therapeutics.
KW - Cartilage
KW - CCN family
KW - Megakaryocyte
KW - Platelet
KW - Regeneration
UR - http://www.scopus.com/inward/record.url?scp=84964433333&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84964433333&partnerID=8YFLogxK
U2 - 10.3109/14397595.2016.1155255
DO - 10.3109/14397595.2016.1155255
M3 - Article
C2 - 26915735
AN - SCOPUS:84964433333
SP - 1
EP - 10
JO - Modern Rheumatology
JF - Modern Rheumatology
SN - 1439-7595
ER -