Involvement of MAPSs in ICAM-1 expression in glomerular endothelial cells in diabetic nephropathy

Naomi Watanabe, Kenichi Shikata, Yasushi Shikata, Kei Sarai, Kazuyoshi Omori, Ryo Kodera, Chikage Sato, Jun Wada, Hirofumi Makino

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Inflammatory processes are involved in the pathogenesis of diabetic nephropathy. The aim of this study was to clarify the role of mitogen-activated protein kinase (MAPK) pathways for induction of intercellular adhesion molecule-1 (ICAM-1) expression in glomerular endothelial cells under diabetic conditions. We examined the expression of ICAM-1 in the kidneys of experimental diabetic rats. Human glomerular endothelial cells (GE cells) were exposed to normal glucose concentration, high glucose concentration (HG), or high mannitol concentration (HM), and then the expression of the ICAM-1 protein and the phosphorylation of the 3 subfamilies of mitogen-activated protein kinase (MAPK) were determined using Western blot analysis. Next, to evaluate the involvement of MAPKs in HG- or HM-induced ICAM-1 expression, we preincubated GE cells with the inhibitors for ERK, p38 or JNK lh prior to the application of glucose or mannitol. Expression of ICAM-1 was increased in the glomeruli of diabetic rats. Both HG and HM induced ICAM-1 expression and phosphorylation of ERK1/2, p38 and JNK in GE cells. Expression of ICAM-1 was significantly attenuated by inhibitors of ERK, p38 and JNK. We conclude that activation of ERK1/2, p38 and JNK cascades may be involved in ICAM-1 expression in glomerular endothelial cells under diabetic conditions.

Original languageEnglish
Pages (from-to)247-257
Number of pages11
JournalActa medica Okayama
Volume65
Issue number4
Publication statusPublished - Aug 2011

Keywords

  • Diabetic nephropathy
  • ERK
  • ICAM-1
  • JNK
  • P 38 MAPK

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Involvement of MAPSs in ICAM-1 expression in glomerular endothelial cells in diabetic nephropathy'. Together they form a unique fingerprint.

  • Cite this

    Watanabe, N., Shikata, K., Shikata, Y., Sarai, K., Omori, K., Kodera, R., Sato, C., Wada, J., & Makino, H. (2011). Involvement of MAPSs in ICAM-1 expression in glomerular endothelial cells in diabetic nephropathy. Acta medica Okayama, 65(4), 247-257.