Involvement of iron oxidation enzyme system in sulfur oxidation of Acidithiobacillus ferrooxidans ATCC 23270

Taher M. Taha, Tadayoshi Kanao, Fumiaki Takeuchi, Tsuyoshi Sugio

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

Growth of A. ferrooxidans ATCC 23270 cells in sulfur medium with 0.005% ferric sulfate for 3, 4, 5, 6, 7 and 10 days gave the maximum growth yield of 45, 58, 76, 86, 90 and 95 mg protein per liter medium, respectively. Iron oxidase activities of 1-, 2- and 3- day-cultured cells on sulfur with 0.005% ferric sulfate (3.4, 3.5 and 0.8 μmol Fe2+ oxidized/mg protein/min) were approximately 68, 70 and 16% of iron-grown ATCC 23270 cells (5.0 μmol/mg protein/min). In contrast iron oxidase activities of 1-, 2- and 3-day cultured cells on sulfur without iron (4.9, 3.8 and 2.7 umol Fe 2+ oxidized/mg protein/min) were approximately 98, 76 and 54% of the iron oxidase activity observed in iron-grown ATCC 23270 cell. SFORase activities of 3 day-cultured cell on sulfur with and without ferric sulfate (0.62 and 0.31 umol Fe2+ produced/mg protein/min) were approximately 20 and 10 fold higher than that of iron-grown cell (0.03 μmol Fe2+ produced/mg protein/min). Both iron oxidase and SFORase activities increased at early-log phase and decreased at late-lag phase during growth of the strain on sulfur with or without Fe3+. The plasma membranes which had iron oxidase activity were prepared not only from iron-grown cells but also sulfur-grown cells. Iron oxidase activities of the plasma membranes prepared from sulfur- and iron-grown cells were 3.6 and 4.5 nmol Fe2+ oxidized per mg protein per min. These results suggest that iron oxidation enzyme system has a role in part in the energy generation of this bacterium from sulfur.

Original languageEnglish
Title of host publicationAdvanced Materials Research
Pages443-446
Number of pages4
Volume20-21
Publication statusPublished - 2007
Event17th International Biohydrometallurgy Symposium, IBS 2007 - Frankfurt am Main, Germany
Duration: Sep 2 2007Sep 5 2007

Publication series

NameAdvanced Materials Research
Volume20-21
ISSN (Print)10226680

Other

Other17th International Biohydrometallurgy Symposium, IBS 2007
CountryGermany
CityFrankfurt am Main
Period9/2/079/5/07

Fingerprint

Sulfur
Enzymes
Iron
Oxidation
Proteins
Cells
Cell membranes
Oxidoreductases
Bacteria

Keywords

  • Acidithiobacillus ferrooxidans
  • Iron oxidase
  • Sulfur oxidation

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Taha, T. M., Kanao, T., Takeuchi, F., & Sugio, T. (2007). Involvement of iron oxidation enzyme system in sulfur oxidation of Acidithiobacillus ferrooxidans ATCC 23270. In Advanced Materials Research (Vol. 20-21, pp. 443-446). (Advanced Materials Research; Vol. 20-21).

Involvement of iron oxidation enzyme system in sulfur oxidation of Acidithiobacillus ferrooxidans ATCC 23270. / Taha, Taher M.; Kanao, Tadayoshi; Takeuchi, Fumiaki; Sugio, Tsuyoshi.

Advanced Materials Research. Vol. 20-21 2007. p. 443-446 (Advanced Materials Research; Vol. 20-21).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Taha, TM, Kanao, T, Takeuchi, F & Sugio, T 2007, Involvement of iron oxidation enzyme system in sulfur oxidation of Acidithiobacillus ferrooxidans ATCC 23270. in Advanced Materials Research. vol. 20-21, Advanced Materials Research, vol. 20-21, pp. 443-446, 17th International Biohydrometallurgy Symposium, IBS 2007, Frankfurt am Main, Germany, 9/2/07.
Taha TM, Kanao T, Takeuchi F, Sugio T. Involvement of iron oxidation enzyme system in sulfur oxidation of Acidithiobacillus ferrooxidans ATCC 23270. In Advanced Materials Research. Vol. 20-21. 2007. p. 443-446. (Advanced Materials Research).
Taha, Taher M. ; Kanao, Tadayoshi ; Takeuchi, Fumiaki ; Sugio, Tsuyoshi. / Involvement of iron oxidation enzyme system in sulfur oxidation of Acidithiobacillus ferrooxidans ATCC 23270. Advanced Materials Research. Vol. 20-21 2007. pp. 443-446 (Advanced Materials Research).
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abstract = "Growth of A. ferrooxidans ATCC 23270 cells in sulfur medium with 0.005{\%} ferric sulfate for 3, 4, 5, 6, 7 and 10 days gave the maximum growth yield of 45, 58, 76, 86, 90 and 95 mg protein per liter medium, respectively. Iron oxidase activities of 1-, 2- and 3- day-cultured cells on sulfur with 0.005{\%} ferric sulfate (3.4, 3.5 and 0.8 μmol Fe2+ oxidized/mg protein/min) were approximately 68, 70 and 16{\%} of iron-grown ATCC 23270 cells (5.0 μmol/mg protein/min). In contrast iron oxidase activities of 1-, 2- and 3-day cultured cells on sulfur without iron (4.9, 3.8 and 2.7 umol Fe 2+ oxidized/mg protein/min) were approximately 98, 76 and 54{\%} of the iron oxidase activity observed in iron-grown ATCC 23270 cell. SFORase activities of 3 day-cultured cell on sulfur with and without ferric sulfate (0.62 and 0.31 umol Fe2+ produced/mg protein/min) were approximately 20 and 10 fold higher than that of iron-grown cell (0.03 μmol Fe2+ produced/mg protein/min). Both iron oxidase and SFORase activities increased at early-log phase and decreased at late-lag phase during growth of the strain on sulfur with or without Fe3+. The plasma membranes which had iron oxidase activity were prepared not only from iron-grown cells but also sulfur-grown cells. Iron oxidase activities of the plasma membranes prepared from sulfur- and iron-grown cells were 3.6 and 4.5 nmol Fe2+ oxidized per mg protein per min. These results suggest that iron oxidation enzyme system has a role in part in the energy generation of this bacterium from sulfur.",
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N2 - Growth of A. ferrooxidans ATCC 23270 cells in sulfur medium with 0.005% ferric sulfate for 3, 4, 5, 6, 7 and 10 days gave the maximum growth yield of 45, 58, 76, 86, 90 and 95 mg protein per liter medium, respectively. Iron oxidase activities of 1-, 2- and 3- day-cultured cells on sulfur with 0.005% ferric sulfate (3.4, 3.5 and 0.8 μmol Fe2+ oxidized/mg protein/min) were approximately 68, 70 and 16% of iron-grown ATCC 23270 cells (5.0 μmol/mg protein/min). In contrast iron oxidase activities of 1-, 2- and 3-day cultured cells on sulfur without iron (4.9, 3.8 and 2.7 umol Fe 2+ oxidized/mg protein/min) were approximately 98, 76 and 54% of the iron oxidase activity observed in iron-grown ATCC 23270 cell. SFORase activities of 3 day-cultured cell on sulfur with and without ferric sulfate (0.62 and 0.31 umol Fe2+ produced/mg protein/min) were approximately 20 and 10 fold higher than that of iron-grown cell (0.03 μmol Fe2+ produced/mg protein/min). Both iron oxidase and SFORase activities increased at early-log phase and decreased at late-lag phase during growth of the strain on sulfur with or without Fe3+. The plasma membranes which had iron oxidase activity were prepared not only from iron-grown cells but also sulfur-grown cells. Iron oxidase activities of the plasma membranes prepared from sulfur- and iron-grown cells were 3.6 and 4.5 nmol Fe2+ oxidized per mg protein per min. These results suggest that iron oxidation enzyme system has a role in part in the energy generation of this bacterium from sulfur.

AB - Growth of A. ferrooxidans ATCC 23270 cells in sulfur medium with 0.005% ferric sulfate for 3, 4, 5, 6, 7 and 10 days gave the maximum growth yield of 45, 58, 76, 86, 90 and 95 mg protein per liter medium, respectively. Iron oxidase activities of 1-, 2- and 3- day-cultured cells on sulfur with 0.005% ferric sulfate (3.4, 3.5 and 0.8 μmol Fe2+ oxidized/mg protein/min) were approximately 68, 70 and 16% of iron-grown ATCC 23270 cells (5.0 μmol/mg protein/min). In contrast iron oxidase activities of 1-, 2- and 3-day cultured cells on sulfur without iron (4.9, 3.8 and 2.7 umol Fe 2+ oxidized/mg protein/min) were approximately 98, 76 and 54% of the iron oxidase activity observed in iron-grown ATCC 23270 cell. SFORase activities of 3 day-cultured cell on sulfur with and without ferric sulfate (0.62 and 0.31 umol Fe2+ produced/mg protein/min) were approximately 20 and 10 fold higher than that of iron-grown cell (0.03 μmol Fe2+ produced/mg protein/min). Both iron oxidase and SFORase activities increased at early-log phase and decreased at late-lag phase during growth of the strain on sulfur with or without Fe3+. The plasma membranes which had iron oxidase activity were prepared not only from iron-grown cells but also sulfur-grown cells. Iron oxidase activities of the plasma membranes prepared from sulfur- and iron-grown cells were 3.6 and 4.5 nmol Fe2+ oxidized per mg protein per min. These results suggest that iron oxidation enzyme system has a role in part in the energy generation of this bacterium from sulfur.

KW - Acidithiobacillus ferrooxidans

KW - Iron oxidase

KW - Sulfur oxidation

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