Involvement of intracellular oxidative stress-sensitive pathway in phloxine B-induced photocytotoxicity in human T lymphocytic leukemia cells

Hang Qi, Beiwei Zhu, Naomi Abe, Yuko Shin, Yoshiyuki Murata, Yoshimasa Nakamura

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We investigated the molecular mechanisms underlying phloxine B (PhB)-induced photocytotoxicity in human T lymphocytic leukemia Jurkat cells. In addition to apoptosis-related biochemical events, photo-irradiated PhB generated intracellular reactive oxygen species (ROS), induced phosphorylation of c-Jun- N-terminal kinase (JNK) in an oxidative stress-dependent manner and up-regulated the gene expression of interferon (IFN)-γ, an inducer of diverse apoptosis-related molecules in activated T cells. PhB-induced apoptosis was significantly inhibited by N-acetyl-l-cysteine, but not by catalase, indicating that ROS generation occurred intracellularly, and by SP600125 and AG490, specific inhibitors of JNK and IFN-γ signaling, respectively, confirming their roles in the apoptotic pathway. IFN-γ up-regulation was also inhibited by SP600125, indicating that it was downstream of JNK activation. These results suggest that PhB-induced apoptosis in Jurkat cells partially involves the intracellular oxidative stress-sensitive and T cell-specific IFN-γ pathway. These data present a novel insight into the mechanisms of photocytotoxicity induced by artificial food colorants in human T lymphocytic leukemia cells.

Original languageEnglish
Pages (from-to)1841-1847
Number of pages7
JournalFood and Chemical Toxicology
Volume50
Issue number6
DOIs
Publication statusPublished - Jun 2012

Fingerprint

Eosine I Bluish
T-Cell Leukemia
lymphocytic leukemia
Oxidative stress
interferons
Oxidative Stress
oxidative stress
apoptosis
Interferons
Apoptosis
Jurkat Cells
T-cells
reactive oxygen species
Synthetic foods
Reactive Oxygen Species
phosphotransferases (kinases)
Phosphotransferases
Food Coloring Agents
T-lymphocytes
Acetylcysteine

Keywords

  • IFN-γ
  • JNK
  • Jurkat cells
  • Phloxine B
  • Photocytotoxicity

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Involvement of intracellular oxidative stress-sensitive pathway in phloxine B-induced photocytotoxicity in human T lymphocytic leukemia cells. / Qi, Hang; Zhu, Beiwei; Abe, Naomi; Shin, Yuko; Murata, Yoshiyuki; Nakamura, Yoshimasa.

In: Food and Chemical Toxicology, Vol. 50, No. 6, 06.2012, p. 1841-1847.

Research output: Contribution to journalArticle

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