Involvement of granulysin-producing T cells in the development of superficial microbial folliculitis

Takashi Oono, Shin Morizane, Osamu Yamasaki, Y. Shirafuji, W. K. Huh, H. Akiyama, K. Iwatsuki

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Granulysin is a recently identified antimicrobial protein expressed on cytotoxic T cells, natural killer (NK) cells and NKT cells. It has been shown that granulysin contributes to the defence mechanisms against mycobacterial infection. Superficial microbial folliculitis is a common skin disease. In a previous report, we showed that, as a first line of defence, α-defensin, a human neutrophil peptide, and β-defensin (human β-defensin-2) were expressed in infiltrating neutrophils and in lesional epidermal keratinocytes, respectively, in superficial folliculitis. As we also observed many infiltrating lymphocytes in lesional dermis, we hypothesized that infiltrating lymphocytes may possess antimicrobial substances, such as granulysin, and play a role in the defence mechanism as a second line of defence. Objectives: Seven specimens of superficial microbial folliculitis diagnosed clinically and histologically were examined by means of immunohistochemistry. To identify the phenotype of cells expressing granulysin, confocal laser microscopic examination was performed. Results: A dense lymphoid cell infiltrate was observed in pustules, in the perivascular regions. A large number of these lymphoid cells were positive for granulysin. Phenotypically, cells consisted of CD3+ T cells, CD8+ T cells and UCHL-1+ T cells. CD20+ cells and CD56+ cells were not observed. Microscopic examination with a confocal laser showed that the lymphocytes producing granulysin were CD3+ and CD4+ T cells but not CD8+ T cells. Conclusions: We showed that many granulysin-bearing T cells infiltrated affected follicles and perilesional dermis in superficial microbial folliculitis. However, few granulysin-positive lymphoid cells were observed in sterile pustular lesions. Our observations indicated that adaptive immunity such as granulysin, a lymphocyte-produced antimicrobial protein, may play an important role in the cutaneous defence mechanism.

Original languageEnglish
Pages (from-to)904-909
Number of pages6
JournalBritish Journal of Dermatology
Volume150
Issue number5
DOIs
Publication statusPublished - May 2004

Fingerprint

Folliculitis
Lymphocytes
T-Lymphocytes
Defensins
Dermis
Lasers
Natural Killer T-Cells
Adaptive Immunity
Keratinocytes
Skin Diseases
Natural Killer Cells
Proteins
Neutrophils
Immunohistochemistry
Phenotype
Skin
Infection

Keywords

  • Adaptive immunity
  • CD4+ T cell
  • Folliculitis
  • Granulysin
  • Innate immunity

ASJC Scopus subject areas

  • Dermatology

Cite this

Involvement of granulysin-producing T cells in the development of superficial microbial folliculitis. / Oono, Takashi; Morizane, Shin; Yamasaki, Osamu; Shirafuji, Y.; Huh, W. K.; Akiyama, H.; Iwatsuki, K.

In: British Journal of Dermatology, Vol. 150, No. 5, 05.2004, p. 904-909.

Research output: Contribution to journalArticle

@article{d3fad372cbc74bffaa10f12683f31664,
title = "Involvement of granulysin-producing T cells in the development of superficial microbial folliculitis",
abstract = "Background: Granulysin is a recently identified antimicrobial protein expressed on cytotoxic T cells, natural killer (NK) cells and NKT cells. It has been shown that granulysin contributes to the defence mechanisms against mycobacterial infection. Superficial microbial folliculitis is a common skin disease. In a previous report, we showed that, as a first line of defence, α-defensin, a human neutrophil peptide, and β-defensin (human β-defensin-2) were expressed in infiltrating neutrophils and in lesional epidermal keratinocytes, respectively, in superficial folliculitis. As we also observed many infiltrating lymphocytes in lesional dermis, we hypothesized that infiltrating lymphocytes may possess antimicrobial substances, such as granulysin, and play a role in the defence mechanism as a second line of defence. Objectives: Seven specimens of superficial microbial folliculitis diagnosed clinically and histologically were examined by means of immunohistochemistry. To identify the phenotype of cells expressing granulysin, confocal laser microscopic examination was performed. Results: A dense lymphoid cell infiltrate was observed in pustules, in the perivascular regions. A large number of these lymphoid cells were positive for granulysin. Phenotypically, cells consisted of CD3+ T cells, CD8+ T cells and UCHL-1+ T cells. CD20+ cells and CD56+ cells were not observed. Microscopic examination with a confocal laser showed that the lymphocytes producing granulysin were CD3+ and CD4+ T cells but not CD8+ T cells. Conclusions: We showed that many granulysin-bearing T cells infiltrated affected follicles and perilesional dermis in superficial microbial folliculitis. However, few granulysin-positive lymphoid cells were observed in sterile pustular lesions. Our observations indicated that adaptive immunity such as granulysin, a lymphocyte-produced antimicrobial protein, may play an important role in the cutaneous defence mechanism.",
keywords = "Adaptive immunity, CD4+ T cell, Folliculitis, Granulysin, Innate immunity",
author = "Takashi Oono and Shin Morizane and Osamu Yamasaki and Y. Shirafuji and Huh, {W. K.} and H. Akiyama and K. Iwatsuki",
year = "2004",
month = "5",
doi = "10.1111/j.1365-2133.2004.05925.x",
language = "English",
volume = "150",
pages = "904--909",
journal = "British Journal of Dermatology",
issn = "0007-0963",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Involvement of granulysin-producing T cells in the development of superficial microbial folliculitis

AU - Oono, Takashi

AU - Morizane, Shin

AU - Yamasaki, Osamu

AU - Shirafuji, Y.

AU - Huh, W. K.

AU - Akiyama, H.

AU - Iwatsuki, K.

PY - 2004/5

Y1 - 2004/5

N2 - Background: Granulysin is a recently identified antimicrobial protein expressed on cytotoxic T cells, natural killer (NK) cells and NKT cells. It has been shown that granulysin contributes to the defence mechanisms against mycobacterial infection. Superficial microbial folliculitis is a common skin disease. In a previous report, we showed that, as a first line of defence, α-defensin, a human neutrophil peptide, and β-defensin (human β-defensin-2) were expressed in infiltrating neutrophils and in lesional epidermal keratinocytes, respectively, in superficial folliculitis. As we also observed many infiltrating lymphocytes in lesional dermis, we hypothesized that infiltrating lymphocytes may possess antimicrobial substances, such as granulysin, and play a role in the defence mechanism as a second line of defence. Objectives: Seven specimens of superficial microbial folliculitis diagnosed clinically and histologically were examined by means of immunohistochemistry. To identify the phenotype of cells expressing granulysin, confocal laser microscopic examination was performed. Results: A dense lymphoid cell infiltrate was observed in pustules, in the perivascular regions. A large number of these lymphoid cells were positive for granulysin. Phenotypically, cells consisted of CD3+ T cells, CD8+ T cells and UCHL-1+ T cells. CD20+ cells and CD56+ cells were not observed. Microscopic examination with a confocal laser showed that the lymphocytes producing granulysin were CD3+ and CD4+ T cells but not CD8+ T cells. Conclusions: We showed that many granulysin-bearing T cells infiltrated affected follicles and perilesional dermis in superficial microbial folliculitis. However, few granulysin-positive lymphoid cells were observed in sterile pustular lesions. Our observations indicated that adaptive immunity such as granulysin, a lymphocyte-produced antimicrobial protein, may play an important role in the cutaneous defence mechanism.

AB - Background: Granulysin is a recently identified antimicrobial protein expressed on cytotoxic T cells, natural killer (NK) cells and NKT cells. It has been shown that granulysin contributes to the defence mechanisms against mycobacterial infection. Superficial microbial folliculitis is a common skin disease. In a previous report, we showed that, as a first line of defence, α-defensin, a human neutrophil peptide, and β-defensin (human β-defensin-2) were expressed in infiltrating neutrophils and in lesional epidermal keratinocytes, respectively, in superficial folliculitis. As we also observed many infiltrating lymphocytes in lesional dermis, we hypothesized that infiltrating lymphocytes may possess antimicrobial substances, such as granulysin, and play a role in the defence mechanism as a second line of defence. Objectives: Seven specimens of superficial microbial folliculitis diagnosed clinically and histologically were examined by means of immunohistochemistry. To identify the phenotype of cells expressing granulysin, confocal laser microscopic examination was performed. Results: A dense lymphoid cell infiltrate was observed in pustules, in the perivascular regions. A large number of these lymphoid cells were positive for granulysin. Phenotypically, cells consisted of CD3+ T cells, CD8+ T cells and UCHL-1+ T cells. CD20+ cells and CD56+ cells were not observed. Microscopic examination with a confocal laser showed that the lymphocytes producing granulysin were CD3+ and CD4+ T cells but not CD8+ T cells. Conclusions: We showed that many granulysin-bearing T cells infiltrated affected follicles and perilesional dermis in superficial microbial folliculitis. However, few granulysin-positive lymphoid cells were observed in sterile pustular lesions. Our observations indicated that adaptive immunity such as granulysin, a lymphocyte-produced antimicrobial protein, may play an important role in the cutaneous defence mechanism.

KW - Adaptive immunity

KW - CD4+ T cell

KW - Folliculitis

KW - Granulysin

KW - Innate immunity

UR - http://www.scopus.com/inward/record.url?scp=3042823931&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042823931&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2133.2004.05925.x

DO - 10.1111/j.1365-2133.2004.05925.x

M3 - Article

C2 - 15149502

AN - SCOPUS:3042823931

VL - 150

SP - 904

EP - 909

JO - British Journal of Dermatology

JF - British Journal of Dermatology

SN - 0007-0963

IS - 5

ER -