Involvement of galectin-9 in guinea pig allergic airway inflammation

Hitomi Yamamoto, Yumiko Kashio, Hiroki Shoji, Rika Shinonaga, Teizo Yoshimura, Nozomu Nishi, Takeshi Nabe, Takanori Nakamura, Shigekatsu Kohno, Mitsuomi Hirashima

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: There is little information about the involvement of galectin-9 (Gal-9) in allergic inflammation. Thus, we investigated the role of Gal-9 in asthma model guinea pigs. Methods: Airway resistance (Raw) was measured using a double-flow plethysmograph system. Gal-9 expression in the lung was assessed by Western blot and immunohistochemistry. Eosinophil chemotactic activity was evaluated in a chamber containing a polyvinylpyrolidone-free membrane. Cell apoptosis was analyzed on a flowcytometry with propidium iodide. Results: In cloning guinea pig Gal-9 we identified three isoforms that differ only in the length of their linker peptides, just as with human Gal-9. Guinea pig Gal-9 was found to be a chemoattractant for eosinophils and to promote induction of apoptosis in sensitized but not non-sensitized T lymphocytes. In allergic airway hypersensitivity model, a low level of Gal-9 expression was observed in the nonsensitized/nonchallenged group, but upregulation was detected at 7 h after challenge and sustained up to 24 h. Such upregulation correlated with elevation of eosinophil peroxidase activity but not with increased R aw. Conclusions: The present results provide evidence that Gal-9 is not involved in airway hypersensitivity, but is partly involved in prolonged eosinophil accumulation in the lung.

Original languageEnglish
Pages (from-to)95-105
Number of pages11
JournalInternational Archives of Allergy and Immunology
Volume143
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Jun 2007
Externally publishedYes

Fingerprint

Galectins
Guinea Pigs
Inflammation
Eosinophils
Hypersensitivity
Up-Regulation
Eosinophil Peroxidase
Apoptosis
Lung
Airway Resistance
Propidium
Chemotactic Factors
Organism Cloning
Protein Isoforms
Asthma
Western Blotting
Immunohistochemistry
T-Lymphocytes
Peptides
Membranes

Keywords

  • Allergic inflammation
  • Animal model
  • Asthma
  • Galectin-9
  • Guinea pig

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Yamamoto, H., Kashio, Y., Shoji, H., Shinonaga, R., Yoshimura, T., Nishi, N., ... Hirashima, M. (2007). Involvement of galectin-9 in guinea pig allergic airway inflammation. International Archives of Allergy and Immunology, 143(SUPPL. 1), 95-105. https://doi.org/10.1159/000101414

Involvement of galectin-9 in guinea pig allergic airway inflammation. / Yamamoto, Hitomi; Kashio, Yumiko; Shoji, Hiroki; Shinonaga, Rika; Yoshimura, Teizo; Nishi, Nozomu; Nabe, Takeshi; Nakamura, Takanori; Kohno, Shigekatsu; Hirashima, Mitsuomi.

In: International Archives of Allergy and Immunology, Vol. 143, No. SUPPL. 1, 06.2007, p. 95-105.

Research output: Contribution to journalArticle

Yamamoto, H, Kashio, Y, Shoji, H, Shinonaga, R, Yoshimura, T, Nishi, N, Nabe, T, Nakamura, T, Kohno, S & Hirashima, M 2007, 'Involvement of galectin-9 in guinea pig allergic airway inflammation', International Archives of Allergy and Immunology, vol. 143, no. SUPPL. 1, pp. 95-105. https://doi.org/10.1159/000101414
Yamamoto, Hitomi ; Kashio, Yumiko ; Shoji, Hiroki ; Shinonaga, Rika ; Yoshimura, Teizo ; Nishi, Nozomu ; Nabe, Takeshi ; Nakamura, Takanori ; Kohno, Shigekatsu ; Hirashima, Mitsuomi. / Involvement of galectin-9 in guinea pig allergic airway inflammation. In: International Archives of Allergy and Immunology. 2007 ; Vol. 143, No. SUPPL. 1. pp. 95-105.
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