Involvement of EphA2 in head and neck squamous cell carcinoma: mRNA expression, loss of heterozygosity and immunohistochemical studies

Rosario S. Rivera, Mehmet Gunduz, Hitoshi Nagatsuka, Esra Gunduz, Beyhan Cengiz, Kunihiro Fukushima, Levent Bekir Beder, Davut Pehlivan, Noboru Yamanaka, Kenji Shimizu, Noriyuki Nagai

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

EphA2 is a 130-kDa transmembrane protein primarily found in adult human epithelial cells and is a member of one of the largest receptor tyrosine kinases. It is located on 1p36.1, a genetic hot spot in cancer. EphA2 over-expression has been observed in aggressive solid tumors and its potential role in tumorigenesis, which includes cell growth, survival, migration and angiogenesis have been reported. However, the role of EphA2 remains unknown in head and neck cancer. In this study, we investigated the genetic profile of EphA2 in primary head and neck squamous cell carcinoma (HNSCC) by determining mRNA level, status of loss of heterozygosity and protein expression. mRNA expression was also correlated with clinicopathological data. Infrequent loss of heterozygosity (20%) was observed, though a 10-fold increase of mRNA expression in tumors compared to normal tissues was noted. A significant number of samples with normal to high mRNA expression was observed among patients with regional metastasis, with T3-T4 tumor size and with moderate to poor differentiation. However, statistical studies did not show any correlation between mRNA expression and any of the clinicopathological parameters. Tumor cells expressed EphA2 protein, but only weakly. These results suggest thate EphA2 might be involved in the early development of HNSCC although not directly responsible for its progression.

Original languageEnglish
Pages (from-to)1079-1084
Number of pages6
JournalOncology Reports
Volume19
Issue number5
Publication statusPublished - May 2008

Fingerprint

Loss of Heterozygosity
Messenger RNA
Neoplasms
EphA2 Receptor
Receptor Protein-Tyrosine Kinases
Head and Neck Neoplasms
Cell Survival
Carcinogenesis
Proteins
Epithelial Cells
Carcinoma, squamous cell of head and neck
Neoplasm Metastasis
Growth

Keywords

  • EphA2
  • Head and neck squamous cell carcinoma
  • Immunohistochemistry
  • Loss of heterozygosity
  • mRNA expression

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Rivera, R. S., Gunduz, M., Nagatsuka, H., Gunduz, E., Cengiz, B., Fukushima, K., ... Nagai, N. (2008). Involvement of EphA2 in head and neck squamous cell carcinoma: mRNA expression, loss of heterozygosity and immunohistochemical studies. Oncology Reports, 19(5), 1079-1084.

Involvement of EphA2 in head and neck squamous cell carcinoma : mRNA expression, loss of heterozygosity and immunohistochemical studies. / Rivera, Rosario S.; Gunduz, Mehmet; Nagatsuka, Hitoshi; Gunduz, Esra; Cengiz, Beyhan; Fukushima, Kunihiro; Beder, Levent Bekir; Pehlivan, Davut; Yamanaka, Noboru; Shimizu, Kenji; Nagai, Noriyuki.

In: Oncology Reports, Vol. 19, No. 5, 05.2008, p. 1079-1084.

Research output: Contribution to journalArticle

Rivera, RS, Gunduz, M, Nagatsuka, H, Gunduz, E, Cengiz, B, Fukushima, K, Beder, LB, Pehlivan, D, Yamanaka, N, Shimizu, K & Nagai, N 2008, 'Involvement of EphA2 in head and neck squamous cell carcinoma: mRNA expression, loss of heterozygosity and immunohistochemical studies', Oncology Reports, vol. 19, no. 5, pp. 1079-1084.
Rivera, Rosario S. ; Gunduz, Mehmet ; Nagatsuka, Hitoshi ; Gunduz, Esra ; Cengiz, Beyhan ; Fukushima, Kunihiro ; Beder, Levent Bekir ; Pehlivan, Davut ; Yamanaka, Noboru ; Shimizu, Kenji ; Nagai, Noriyuki. / Involvement of EphA2 in head and neck squamous cell carcinoma : mRNA expression, loss of heterozygosity and immunohistochemical studies. In: Oncology Reports. 2008 ; Vol. 19, No. 5. pp. 1079-1084.
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