Involvement of cytochrome bs in the metabolism of tetrachlorobiphenyls catalyzed by CYP2B1 and CYP1A1

Kimihiko Matsusue, Noritaka Ariyoshi, Kazuta Oguri, Nobuyuki Koga, Hidetoshi Yoshimura

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

The role of cytochrome b5 in the cytochrome P450 (CYP)-dependent hydroxylation of tetrachlorobiphenyl (TCB) isomers was examined using a reconstituted mixed function oxygenase (MFO) system containing purified CYP2B1 or 1A1, and rat liver microsomes. Hydroxylations of 2,2′,5,5′- and 3,3′,4,4′-TCBs were catalyzed mainly by CYP2B1 and 1A1, respectively, in the reconstituted MFO system and those of 2,3′,4′,5- and 2,3′,4,4′-TCBs were mediated by both cytochrome P450 systems. The activity toward 2,2′,5,5′- and 2,3′,4′,5-TCB was significantly increased 6.5- and 5.5-fold, respectively, by addition of cytochrome b5 in the reconstituted MFO system containing of CYP2B1. Either hydroxylation activity toward 2,3′,4,4′-TCB with the CYP2B1 system was very low or decreased by addition of cytochrome b5. These results suggest that the involvement of cytochrome b5 to the hydroxylation of TCBs is dependent on the TCB congener being metabolized, and the cytochrome P450 isoform involved in its metabolism.

Original languageEnglish
Pages (from-to)517-523
Number of pages7
JournalChemosphere
Volume32
Issue number3
DOIs
Publication statusPublished - Feb 1996
Externally publishedYes

Keywords

  • Cytochrome P450
  • Cytochrome b
  • Polychlorinated biphenyl
  • Tetrachlorobiphenyl (TCB)

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

Fingerprint Dive into the research topics of 'Involvement of cytochrome b<sub>s</sub> in the metabolism of tetrachlorobiphenyls catalyzed by CYP2B1 and CYP1A1'. Together they form a unique fingerprint.

Cite this