Involvement of cyclin-dependent kinase 5/p35nck5a in the synaptic reorganization of rat hippocampus during kindling progression

K. Tomizawa, X. H. Cai, A. Morikawi, M. Matsushita, H. Matsui

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

To test the hypothesis that a complex of cyclin-dependent kinase 5 (Cdk5) and p35nck5a plays an important role in sprouting in the kindling rat hippocampus, we studied the changes in kinase activity, expression level and subcellular localization during kindling progression. The kinase activity in kindling rats was significantly higher than that in normal rats. The changes in kinase activity coincided with those of the p35nck5a expression in kindling rats. In contrast, the expression of Cdk5 was constant at all stages of kindling. Subcellular localization of Cdk5, however, changed markedly in the hippocampal neurons during kindling progression Cdk5 translocated from axon to soma when the kinase activity was high. The phosphorylation level of tau protein was in good agreement with the Cdk5 kinase activity. In contrast, MAP kinase activity was not correlated with tau phosphorylation during kindling progression. These findings suggest that Cdk5/p35nck5a plays an important role in synaptic reorganization, and the translocation of Cdk5 to soma from axons is a crucial regulatory mechanism of kinase activity.

Original languageEnglish
Pages (from-to)525-532
Number of pages8
JournalJapanese journal of physiology
Volume50
Issue number5
DOIs
Publication statusPublished - Dec 1 2000

Keywords

  • Cdk5
  • Neuronal plasticity
  • Sprouting
  • Tau
  • p35

ASJC Scopus subject areas

  • Physiology

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