Involvement of CTGF, a hypertrophic chondrocyte-specific gene product, in tumor angiogenesis

Tsuyoshi Shimo, Tohru Nakanishi, Takashi Nishida, Masahiro Asano, Akira Sasaki, Manabu Kanyama, Takuo Kuboki, Tomohiro Matsumura, Masaharu Takigawa

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Connective tissue growth factor (CTGF) is a potent secreted signaling factor which functions in multiple stages of angiogenesis. In the present study, we examined the role of CTGF in tumor angiogenesis and made the following observations: (1) Histological analysis of human breast cancer (MDA231) cell and human fibrosarcoma (HT1080) cell xenografts in BALB/c nude mice showed a high level of neovascularization. Human squamous cell carcinoma (A431) xenografts induced only a low level of neovascularization. (2) CTGF mRNA was strongly expressed in MDA231 and in HT1080 cells in vivo and in vitro, but not in A431 cells. (3) CTGF protein was markedly produced in MDA231 cells and HT1080 cells and secreted into culture medium, and its production was greater during phases of growth rather than confluency. (4) Production of CTGF in bovine aorta endothelial cells was induced by CTGF, VEGF, bFGF and TGF-β. (5) Neovascularization induced by HT1080 cells or MDA231 cells on chicken chorioallantoic membrane was suppressed in the presence of neutralizing CTGF-specific polyclonal antibody. These results suggest that CTGF regulates progression in tumor angiogenesis and the release or secretion of CTGF from tumor cells is essential for the angiogenesis.

Original languageEnglish
Pages (from-to)315-322
Number of pages8
JournalOncology
Volume61
Issue number4
DOIs
Publication statusPublished - 2001

Fingerprint

Connective Tissue Growth Factor
Chondrocytes
Genes
Neoplasms
Heterografts
Chorioallantoic Membrane
Fibrosarcoma
Nude Mice
Vascular Endothelial Growth Factor A
Culture Media
Aorta
Squamous Cell Carcinoma
Chickens
Endothelial Cells
Breast Neoplasms
Messenger RNA

Keywords

  • Angiogenesis
  • Connective tissue growth factor
  • Neovascularization

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Involvement of CTGF, a hypertrophic chondrocyte-specific gene product, in tumor angiogenesis. / Shimo, Tsuyoshi; Nakanishi, Tohru; Nishida, Takashi; Asano, Masahiro; Sasaki, Akira; Kanyama, Manabu; Kuboki, Takuo; Matsumura, Tomohiro; Takigawa, Masaharu.

In: Oncology, Vol. 61, No. 4, 2001, p. 315-322.

Research output: Contribution to journalArticle

Shimo, Tsuyoshi ; Nakanishi, Tohru ; Nishida, Takashi ; Asano, Masahiro ; Sasaki, Akira ; Kanyama, Manabu ; Kuboki, Takuo ; Matsumura, Tomohiro ; Takigawa, Masaharu. / Involvement of CTGF, a hypertrophic chondrocyte-specific gene product, in tumor angiogenesis. In: Oncology. 2001 ; Vol. 61, No. 4. pp. 315-322.
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